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	Provedor de dados BJMBR (1) Electron. J. Biotechnol. (1) Autor Denapolis,P.M.A. (1) Han,S.W. (1) Li,Liang (1) Li,Xinxin (1) Liu,Hehe (1) Moraes,J.Z. (1) Sacramento,C.B. (1) Sun,Lingli (1) Wang,Haohan (1) Wang,Jiwen (1) Yang,Chao (1) Zheng,Yi (1) Mais... Palavra-chave Transfection efficiency (2) Follistatin (1) Hypoxia (1) Hypoxia-responsive element (1) Ischemic diseases (1) Muscle hypertrophy (1) Normoxia (1) Overexpression (1) SV40-DTS (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2014 (1) 2010 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts Electron. J. Biotechnol. Li,Xinxin; Wang,Jiwen; Liu,Hehe; Wang,Haohan; Sun,Lingli; Yang,Chao; Li,Liang; Zheng,Yi. Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in... Tipo: Journal article Palavras-chave: Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia BJMBR Sacramento,C.B.; Moraes,J.Z.; Denapolis,P.M.A.; Han,S.W.. The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by... Tipo: Info:eu-repo/semantics/article Palavras-chave: SV40-DTS; Hypoxia-responsive element; Transfection efficiency; Normoxia; Hypoxia; Ischemic diseases. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800005 Registros recuperados: 2 Primeira ... 1 ... Última

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