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Registros recuperados: 62
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Biotransformation of grape pomace from Vitis labrusca by Peniophora albobadia LPSC # 285 (Basidiomycota) 42
TRONCOZO,MARÍA I.; FIGOLI,CECILIA B.; FRANCO,MARIO E.E.; MIRÍFICO,MARÍA V.; BOSCH,ALEJANDRA; RAJCHENBERG,MARIO; BALATTI,PEDRO A.; SAPARRAT,MARIO C. N..
Abstract Grape pomace from Vitis labrusca is an important sub-product of the “American table wine” industry. It is recalcitrant to degradation, and its accumulation is a serious problem with negative environmental impacts. We analyzed the ability of five white-rot fungi to transform this residue in-vitro. Mass loss and phenol removal in grape pomace treated with each fungus were compared after 30-day solid-state fermentation. Since Peniophora albobadia isolate LPSC 285 was the fungus that showed the highest degradative ability and the lowest free phenol levels in the residue transformed, we selected this fungus to monitor its effect on this residue after 30, 60, and 90 days of incubation. We analyzed mass loss of the residue caused by the fungus activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Grape pomace; Peniophora albobadia; Transformation; Vitis labrusca.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100704
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Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao 52
Lopes,Francis Julio Fagundes; Queiroz,Marisa Vieira de; Lima,Juliana Oliveira; Silva,Viviane Aline Oliveira; Araújo,Elza Fernandes de.
The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Moniliophthora perniciosa; Transformation; Restriction enzyme.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100004
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Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment 52
Bespalhok Filho,João C.; Kobayashi,Adilson K.; Pereira,Luiz F. P.; Galvão,Rafaelo M.; Vieira,Luiz G. E..
Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transformation; UidA expression; Biolistics; Transgenic citrus.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001
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Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids 56
Schmidt,M.; Cömer,A.; Grey,M.; Brendel,M..
Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Transformation; Hyperresistance to formaldehyde.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004
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Comparative study of wild and transformed salt tolerant bacterial strains on Triticum aestivum growth under salt stress 58
Afrasayab,Shazia; Faisal,Muhammad; Hasnain,Shahida.
Eleven salt tolerant bacteria isolated from different sources (soil, plants) and their transformed strains were used to study their influence on Triticum aestivum var. Inqlab-91 growth under salt (100 mM NaCl) stress. Salt stress caused reduction in germination (19.4%), seedling growth (46%) and fresh weight (39%) in non-inoculated plants. In general, both wild and transformed strains stimulated germination, seedling growth and fresh weight in salt free and salt stressed conditions. At 100 mM NaCl, Staphylococcus xylosus ST-1 caused 25% increments in seedling length over respective control. Soluble protein content significantly enhanced (49%) under salt stress as compared to salt free control. At 100 mM NaCl parental strain PT-5 resulted about 32%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Plasmid; Rhizobacteria; Salinity; Auxin; Transformation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400013
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Glass bead transformation method for gram-positive bacteria 58
Rattanachaikunsopon,Pongsak; Phumkhachorn,Parichat.
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transformation; Gram-positive bacteria; Glass bead.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400025
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The virulence of Streptococcus pneumoniae partially depends on dprA 58
Yu,Yi; Chang,De; Xu,Huiwen; Zhang,Xuelin; Pan,Lei; Xu,Chou; Huang,Bing; Zhou,Hong; Li,Jia; Guo,Jun; Liu,Changting.
Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study was to characterize the effects of the pneumococcal dprA gene on the pathogenesis of S. pneumoniae. To construct a dprA-deficient pneumococcal strain, the dprA gene of the S. pneumoniae strain D39 was inactivated. The virulence of this dprA-deficient strain, designated ΔD39, was compared with that of the wild-type strain by evaluating their respective capabilities to adhere to human pulmonary epithelial cells (PEC-A549) and by analyzing their choline-binding...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus pneumoniae; DprA; Transformation; Virulence.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200225
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Synthesis: Vulnerability, Traps, and Transformations—Long-term Perspectives from Archaeology 7
Schoon, Michael; Arizona State University; michael.schoon@asu.edu; Fabricius, Christo; Nelson Mandela Metropolitan University; christo.fabricius@gmail.com; Anderies, John M.; Arizona State University; m.anderies@asu.edu; Nelson, Margaret; Arizona State University; mnelson@asu.edu.
In this synthesis, we hope to accomplish two things: 1) reflect on how the analysis of the new archaeological cases presented in this special feature adds to previous case studies by revisiting a set of propositions reported in a 2006 special feature, and 2) reflect on four main ideas that are more specific to the archaeological cases: i) societal choices are influenced by robustness–vulnerability trade-offs, ii) there is interplay between robustness–vulnerability trade-offs and robustness–performance trade-offs, iii) societies often get locked in to particular strategies, and iv) multiple positive feedbacks escalate the perceived cost of societal change. We then discuss whether these lock-in traps can be prevented or...
Tipo: Peer-Reviewed Synthesis Palavras-chave: Archaeology; Robustness; Trade-offs; Transformation; Vulnerability.
Ano: 2011
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Resilience: Accounting for the Noncomputable 7
Carpenter, Stephen R; University of Wisconsin; srcarpen@wisc.edu; Folke, Carl; Stockholm University; Beijer Institute; carl.folke@beijer.kva.se; Scheffer, Marten; Wageningen University; Marten.Scheffer@wur.nl; Westley, Frances; University of Waterloo; fwestley@watarts.uwaterloo.ca.
Plans to solve complex environmental problems should always consider the role of surprise. Nevertheless, there is a tendency to emphasize known computable aspects of a problem while neglecting aspects that are unknown and failing to ask questions about them. The tendency to ignore the noncomputable can be countered by considering a wide range of perspectives, encouraging transparency with regard to conflicting viewpoints, stimulating a diversity of models, and managing for the emergence of new syntheses that reorganize fragmentary knowledge.
Tipo: Peer-Reviewed Insight Palavras-chave: Resilience; Adaptation; Transformation; Surprise.
Ano: 2009
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Navigating the Back Loop: Fostering Social Innovation and Transformation in Ecosystem Management 7
Biggs, Reinette; University of Wisconsin-Madison, USA; Stockholm Resilience Centre, Stockholm University, Sweden; oonsie.biggs@stockholmresilience.su.se; Westley, Frances R.; University of Waterloo, Canada; fwestley@uwaterloo.ca; Carpenter, Stephen R.; University of Wisconsin-Madison, USA; srcarpen@wisc.edu.
Addressing the environmental challenges of the 21st century requires substantial changes to the way modern society views and manages ecosystems. In particular, many authors contend that fundamental transformation of the largely sectoral, expert-centered ecosystem-management institutions of modern, Western societies is needed. There is increasing agreement that more adaptive, integrated, collaborative ecosystem-management approaches, interlinked at multiple scales, would improve society’s ability to sustainably manage complex social–ecological systems. Therefore, understanding processes of transformation, and factors that may enable transformation in ecosystem management, has become an active research area. We explore...
Tipo: Peer-Reviewed Insight Palavras-chave: Adaptive cycle; Bridging organization; Co-management; Complex systems; Ecosystem management; Social entrepeneur; Social innovation; Transformation.
Ano: 2010
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Continuity and Change in Social-ecological Systems: the Role of Institutional Resilience 7
Pahl-Wostl, Claudia; Institute for Environmental Systems Research, University of Osnabrueck; pahl@usf.uni-osnabrueck.de.
In recent years recurring political, economic, and environmental crises require questioning and re-evaluating dominant pathways of human development. However, political and economic frameworks seem to encompass deeply rooted resistance to fundamental changes (e.g., global financial crisis, climate change negotiations). In an effort to repair the system as fast as possible, those paradigms, mechanisms, and structures that led into the crisis are perpetuated. Instead of preserving conventional patterns and focusing on continuity, crises could be used as an opportunity for learning, adapting, and entering onto more sustainable pathways. However, there are different ways not only of arguing for sustainable pathways of development but also of conceptualizing...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Institutions; Persistence; Resilience; Transformation; Water governance.
Ano: 2012
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Understanding adaptation and transformation through indigenous practice: the case of the Guna of Panama 7
Apgar, Marina J; Policy, Economic and Social Sciences, WorldFish Center; m.apgar@cgxchange.org; Allen, Will; Learning for Sustainability; willallennz@gmail.com; Moore, Kevin; Faculty of Environment, Society and Design, Lincoln University; kevin.moore@lincoln.ac.nz; Ataria, James; Te Matapuna, Kaupapa Maori Unit, Faculty of Agriculture and Life Sciences, Lincoln University; jamie.ataria@gmail.com.
Resilience is emerging as a promising vehicle for improving management of social-ecological systems that can potentially lead to more sustainable arrangements between environmental and social spheres. Central to an understanding of how to support resilience is the need to understand social change and its links with adaptation and transformation. Our aim is to contribute to insights about and understanding of underlying social dynamics at play in social-ecological systems. We argue that longstanding indigenous practices provide opportunities for investigating processes of adaptation and transformation. We use in-depth analysis of adaptation and transformation through engagement in participatory action research, focusing on the role of cultural and social...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Cultural practice; Guna; Reflection; Resilience; Ritual; Participatory action research; Transformation.
Ano: 2015
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Studying the complexity of change: toward an analytical framework for understanding deliberate social-ecological transformations 7
Moore, Michele-Lee; Department of Geography, University of Victoria; mlmoore@uvic.ca; Tjornbo, Ola; Waterloo Institute of Social Innovation and Resilience, University of Waterloo; ola.tjornbo@gmail.com; Enfors, Elin; Stockholm Resilience Centre, Stockholm University; elin.enfors@stockholmresilience.su.se; Knapp, Corrie; University of Alaska Fairbanks; corrieknapp@yahoo.com; Hodbod, Jennifer; Global Institute of Sustainability, Arizona State University; jennifer.hodbod@asu.edu; Baggio, Jacopo A.; Center for the Study of Institutional Diversity, Arizona State University; jbaggio@asu.edu; Olsson, Per; Stockholm Resilience Centre, Stockholm University; per.olsson@stockholmresilience.su.se; Biggs, Duan; The Centre for Biodiversity and Conservation Science, University of Queensland; ancientantwren@gmail.com.
Faced with numerous seemingly intractable social and environmental challenges, many scholars and practitioners are increasingly interested in understanding how to actively engage and transform the existing systems holding such problems in place. Although a variety of analytical models have emerged in recent years, most emphasize either the social or ecological elements of such transformations rather than their coupled nature. To address this, first we have presented a definition of the core elements of a social-ecological system (SES) that could potentially be altered in a transformation. Second, we drew on insights about transformation from three branches of literature focused on radical change, i.e., social movements, socio-technical transitions, and...
Tipo: Peer-Reviewed Reports Palavras-chave: Resilience; Social-ecological systems; Social innovation; Social movements; Transformation; Transition management.
Ano: 2014
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Building resilient pathways to transformation when “no one is in charge”: insights from Australia's Murray-Darling Basin 7
Abel, Nick; Land and Water, Commonwealth Scientific and Industrial Research Organisation ; nick.abel@csiro.au; Wise, Russell M.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; russell.wise@csiro.au; Colloff, Matthew J.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Matt.Colloff@csiro.au; Walker, Brian H.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; brian.walker@csiro.au; Butler, James R. A.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; james.butler@csiro.au; Ryan, Paul; Australian Resilience Centre; paulryan@internode.on.net; Norman, Chris; Goulburn Broken Catchment Management Authority; chrisn@gbcma.vic.gov.au; Langston, Art; Land and Water, Commonwealth Scientific and Industrial Research Organisation; art.langston@csiro.au; Anderies, John M.; Arizona State University; m.anderies@asu.edu; Gorddard, Russell; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Russell.Gorddard@csiro.au; Dunlop, Michael; Land and Water, Commonwealth Scientific and Industrial Research Organisation; michael.dunlop@csiro.au; O'Connell, Deborah; Land and Water, Commonwealth Scientific and Industrial Research Organisation; deborah.o'connell@csiro.au.
Climate change and its interactions with complex socioeconomic dynamics dictate the need for decision makers to move from incremental adaptation toward transformation as societies try to cope with unprecedented and uncertain change. Developing pathways toward transformation is especially difficult in regions with multiple contested resource uses and rights, with diverse decision makers and rules, and where high uncertainty is generated by differences in stakeholders’ values, understanding of climate change, and ways of adapting. Such a region is the Murray-Darling Basin, Australia, from which we provide insights for developing a process to address these constraints. We present criteria for sequencing actions along adaptation pathways: feasibility...
Tipo: Peer-Reviewed Insight Palavras-chave: Adaptation pathways; Climate change; Collective action; Domain shift; Equity; Irrigation; Resilience; Social conflict; Transformation; Wetlands.
Ano: 2016
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Ecosystem Services Linking Social and Ecological Systems: River Brownification and the Response of Downstream Stakeholders 7
Tuvendal, Magnus; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; magnus.tuvendal@ecology.su.se; Elmqvist, Thomas; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; thomase@ecology.su.se.
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Brownification; Coping; Ecosystem service; Governance; Resilience; Response strategies; Social-ecological system; Transformation.
Ano: 2011
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Multiple pathways to sustainability in the city: the case of San Juan, Puerto Rico 7
I examined the multiple visions of the future of the city that can emerge when city actors and organizations reconfigure themselves to address sustainability. In various cities worldwide, novel ideas, initiatives, and networks are emerging in governance to address social and ecological conditions in urban areas. However, cities can be contested spaces, bringing a plurality of actors, network configurations, preferences, and knowledge that shape the politics over desirable pathways for future development. I used the knowledge-action systems analysis (KASA) approach to examine the frames and knowledge systems influencing how different actors involved in the land governance network of the city of San Juan constructed visions for the future of the city....
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptive capacity; Adaptive governance; Frames; Knowledge-action systems; Networks; San Juan; Sustainable pathways; Transformation; Urban social-ecological systems; Visions.
Ano: 2014
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Exploring institutional adaptive capacity in practice: examining water governance adaptation in Australia 7
Bettini, Yvette; University of Queensland, Institute for Social Science Research; Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; y.bettini@uq.edu.au; Brown, Rebekah R; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Rebekah.Brown@monash.edu; de Haan, Fjalar J; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Fjalar.dehaan@monash.edu.
Adaptive capacity is widely held as a key property of resilient and transformative social-ecological systems. However, current knowledge of the term does not yet address key questions of how to operationalize this system condition to address sustainability challenges through research and policy. This paper explores temporal and agency dimensions of adaptive capacity in practice to better understand how system conditions and attributes enable adaptation. An institutional dynamics lens is employed to systemically examine empirical cases of change in urban water management. Comparative analysis of two Australian cities' drought response is conducted using institutional analysis and qualitative system dynamics mapping techniques. The study finds that three...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptive capacity; Institutions; Resilience; Transformation; Water governance.
Ano: 2015
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Resilience to climate change in a cross-scale tourism governance context: a combined quantitative-qualitative network analysis 7
Luthe, Tobias; University of Applied Sciences Chur, Institute for Tourism and Leisure, Switzerland; University of Freiburg, Centre for Key Qualifications, Germany; info@tobiasluthe.de.
Social systems in mountain regions are exposed to a number of disturbances, such as climate change. Calls for conceptual and practical approaches on how to address climate change have been taken up in the literature. The resilience concept as a comprehensive theory-driven approach to address climate change has only recently increased in importance. Limited research has been undertaken concerning tourism and resilience from a network governance point of view. We analyze tourism supply chain networks with regard to resilience to climate change at the municipal governance scale of three Alpine villages. We compare these with a planned destination management organization (DMO) as a governance entity of the same three municipalities on the regional scale....
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Climate change; Core-periphery integration; Social network analysis; Stakeholder perceptions; Tourism destination; Transformation.
Ano: 2016
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Power Asymmetries in Small-Scale Fisheries: a Barrier to Governance Transformability? 7
Crona, Beatrice; Stockholm Resilience Center, Stockholm University, Sweden; beatrice.crona@stockholmresilience.su.se.
Both global and local environmental problems call for the transformation of many contemporary and unsustainable governance approaches. Therefore, recent interest has sprung up around factors that facilitate and hinder societies from transforming governance of natural resources. Using a social-network approach, we study links between informal power structures and knowledge sharing and consensus building. We examine how this interaction may have affected the (in)ability of a community to move from open-access to some form of collective action for resource management. Individuals occupying central positions in a knowledge network can be instrumental in determining which knowledge and interpretation of ecological signals is most dominant. If the same...
Tipo: Peer-Reviewed Reports Palavras-chave: Comanagement; Governance; Local ecological knowledge; Natural resources; Power; Social networks; Transformation.
Ano: 2010
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From barriers to limits to climate change adaptation: path dependency and the speed of change 7
Barnett, Jon; School of Geography, University of Melbourne; jbarn@unimelb.edu.au; Evans, Louisa S; Geography, College of Life and Environmental Science, University of Exeter; Australian Research Council Centre of Excellence for Coral Reef Studies, James Cook University; louisa.evans@exeter.ac.uk; Gross, Catherine; Fenner School of Environment and Society, Australian National University; catherine.gross@anu.edu.au; Kiem, Anthony S; School of Environmental and Life Sciences, University of Newcastle; anthony.kiem@newcastle.edu.au; Kingsford, Richard T.; School of Biological, Earth and Environmental Sciences, University of New South Wales; richard.kingsford@unsw.edu.au; Palutikof, Jean P.; National Climate Change Adaptation Research Facility, Griffith University; j.palutikof@griffith.edu.au; Pickering, Catherine M; School of Environment, Griffith University; c.pickering@griffith.edu.au; Smithers, Scott G; College of Marine and Environmental Sciences, James Cook University; scott.smithers@jcu.edu.au.
Research on the barriers and limits to climate change adaptation identifies many factors, but describes few processes whereby adaptation is constrained or may indeed fail to avoid catastrophic losses. It often assumes that barriers are by and large distinct from limits to adaptation. We respond to recent calls for comparative studies that are able to further knowledge about the underlying drivers of barriers and limits to adaptation. We compare six cases from across Australia, including those in alpine areas, rivers, reefs, wetlands, small inland communities, and islands, with the aim of identifying common underlying drivers of barriers and limits to adaptation. We find that the path-dependent nature of the institutions that govern natural resources and...
Tipo: Peer-Reviewed Reports Palavras-chave: Communities; Cultures; Ecosystems; Markets; Path dependence; Transformation; Values.
Ano: 2015
Registros recuperados: 62
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