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| Menezes,Kelly Alexsandra Souza; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Martins,Lindete Míria Vieira; Fernandes Júnior,Paulo Ivan. |
| ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina Willd.) nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological nitrogen fixation; “mulungu”; Tree legumes; “Caatinga”; Rhizobia. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100404 |
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| Barros,Felipe Martins do Rêgo; Fracetto,Giselle Gomes Monteiro; Fracetto,Felipe José Cury; Mendes Júnior,José Petrônio; Araújo,Victor Lucas Vieira Prudêncio de; Lira Junior,Mario Andrade. |
| ABSTRACT Intercropping tree legumes with forage grasses in a silvopastoral system can avoid pasture degradation benefiting the soil. In such a system, nitrogen (N) is supplied by symbiosis between legumes and bacteria. However, the pasture quality determines the action of free-living nitrogen-fixing bacteria, which possess nifH genes, which encode nitrogenase enzyme. Ammonium-oxidizing bacteria (AOB), involved in the nitrification step, can be evaluated by specific regions of the 16S rRNA corresponding to AOB. Thus, we investigated the influence of the introduction of tree legumes into a silvopastoral system on the community structure and abundance of total bacteria, diazotrophic bacteria and ammonium-oxidizing bacteria by DGGE (denaturing gradient gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tree legumes; Brachiaria; 16 rRNA; AOB; NifH.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000300281 |
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