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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (2) BJM (1) Autor Ambu,S (1) Bisht,Deepa (1) Ibrahim,Halijah (1) Ibrahim,I (1) Joshi,Beenu (1) Katoch,V.M. (1) Khoon,Too Lay (1) Kumar,Gavish (1) Ponnudurai,G (1) Shankar,Hari (1) Sharma,Prashant (1) Shin Yee,L (1) Singhal,Neelja (1) Vejayan,J (1) Vejayan,Jaya (1) Mais... Palavra-chave Two-dimensional gel electrophoresis (3) Culture filtrate proteins (1) M. tuberculosis (1) Mass spectrometry (1) Protein profile analysis (1) Proteomics (1) SDS-PAGE (1) SDS-TCA procedure (1) Snake venom (1) Snake venoms (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2014 (1) 2010 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis J. Venom. Anim. Toxins incl. Trop. Dis. Vejayan,J; Shin Yee,L; Ponnudurai,G; Ambu,S; Ibrahim,I. Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family - Trimeresurus sumatranus, Tropidolaemus wagleri and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Protein profile analysis; Two-dimensional gel electrophoresis; SDS-PAGE. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400013 Comparative analysis of the venom proteome of four important Malaysian snake species J. Venom. Anim. Toxins incl. Trop. Dis. Vejayan,Jaya; Khoon,Too Lay; Ibrahim,Halijah. BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317 A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis BJM Kumar,Gavish; Shankar,Hari; Bisht,Deepa; Sharma,Prashant; Singhal,Neelja; Katoch,V.M.; Joshi,Beenu. Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids. Tipo: Info:eu-repo/semantics/article Palavras-chave: Culture filtrate proteins; M. tuberculosis; SDS-TCA procedure; Two-dimensional gel electrophoresis. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200005 Registros recuperados: 3 Primeira ... 1 ... Última

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