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	Provedor de dados BJM (2) Autor Astolfi-Filho,Spartaco (2) Braga,Luis Paulo da Silva (1) Chies,Jocelei M. (1) Chies,Jocelei Maria (1) Dias,Ana C. de O. (1) Dias,Ana Christina de Oliveira (1) Maia,Hélio M.M. (1) Maia,Hélio Mauro Moreira (1) Mais... Palavra-chave Type II restriction endonuclease (2) Bacillus licheniformis (1) Bacillus pumilus (1) BliAI (1) BpuAmI (1) ClaI (1) Isoschizomers (1) Neoschizomers (1) SacI (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG¯CTC-3' BJM Chies,Jocelei M.; Dias,Ana C. de O.; Maia,Hélio M.M.; Astolfi-Filho,Spartaco. A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Type II restriction endonuclease; BpuAmI; SacI; Neoschizomers; Bacillus pumilus. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100018 Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis BJM Chies,Jocelei Maria; Dias,Ana Christina de Oliveira; Maia,Hélio Mauro Moreira; Braga,Luis Paulo da Silva; Astolfi-Filho,Spartaco. The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa. Tipo: Info:eu-repo/semantics/article Palavras-chave: Type II restriction endonuclease; BliAI; ClaI; Isoschizomers; Bacillus licheniformis. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400027 Registros recuperados: 2 Primeira ... 1 ... Última

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