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	Provedor de dados BJMBR (1) BJM (1) Autor Alves da Silva,A.V. (1) Amaral,M.S.M.G. (1) Assis,Maria Cristina (1) Bona,M.D. (1) Cavalcante,P.A. (1) Havt,A. (1) Lima,A.A.M. (1) Lima,I.F.N. (1) Medeiros,P.H.Q.S. (1) Nishi,Rebeca (1) Plotkowski,Maria Cristina (1) Prata,M.M.G. (1) Quetz,J.S. (1) Reyes,M.A.V. (1) Ribeiro,S.A. (1) Rodrigues,F.A.P. (1) Rodrigues,T.S. (1) Saliba,Alessandra Mattos (1) Santos,A.K.S. (1) Veras,H.N. (1) Mais... Palavra-chave Type III secretion system (2) Bacterial cytotoxicity (1) Endothelial cells (1) Enteropathogenic Escherichia coli (1) ExoU (1) Intestinal cell migration (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains BJMBR Cavalcante,P.A.; Prata,M.M.G.; Medeiros,P.H.Q.S.; Alves da Silva,A.V.; Quetz,J.S.; Reyes,M.A.V.; Rodrigues,T.S.; Santos,A.K.S.; Ribeiro,S.A.; Veras,H.N.; Bona,M.D.; Amaral,M.S.M.G.; Rodrigues,F.A.P.; Lima,I.F.N.; Havt,A.; Lima,A.A.M.. Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis and necrosis levels... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli; Intestinal cell migration; Type III secretion system. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000602 ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells BJM Saliba,Alessandra Mattos; Nishi,Rebeca; Assis,Maria Cristina; Plotkowski,Maria Cristina. To ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not... Tipo: Info:eu-repo/semantics/article Palavras-chave: Type III secretion system; ExoU; Endothelial cells; Bacterial cytotoxicity. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008 Registros recuperados: 2 Primeira ... 1 ... Última

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