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Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods Scientia Agricola
Duffeck,Maíra Rodrigues; Tibola,Casiane Salete; Guarienti,Eliana Maria; Del Ponte,Emerson Medeiros.
ABSTRACT One hundred commercial wheat grain samples were collected during the 2015 sea-son across 78 municipalities in the states of Paraná (PR), Rio Grande do Sul (RS), and São Paulo (SP), Brazil. Separate subsamples were analyzed for the concentration of deoxynivalenol (DON), zearalenona (ZEA) and ochratoxin A (OTA) mycotoxins using two methods: UHPLC-MS/MS (reference method) and a commercial enzyme-linked immunosorbent assay (ELISA) (AgraQuant®). The OTA mycotoxin was not found in the samples by both methods. DON and ZEA were detected in 55 % and 39 % of the samples by the reference method, with overall mean levels of 795.2 μg kg−1 and 79.78 μg kg−1, respectively. There was a significant and positive correlation (Spearman rank) between DON and ZEA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; UHPLC; ELISA; Trichothecenes; Ochratoxin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400343
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Polyphenols From Cutch Tree ( Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity BABT
Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100303
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Polyphenols From Cutch Tree (Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity BABT
Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100316
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