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Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections BJM
Maluta,Renato Pariz; Stella,Ariel Eurides; Riccardi,Kátia; Rigobelo,Everlon Cid; Marin,José Moacir; Carvalho,Marileda Bonafim; Ávila,Fernando Antonio de.
Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomarkers; Biotyping; ERIC-PCR; UPEC.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100045
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Curli fimbria: an Escherichia coli adhesin associated with human cystitis BJM
Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414
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