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Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae Scientia Agricola
Kava - Cordeiro,V.; Luna - Alves - Lima,E.A.; Azevedo,J.L..
A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Auxotrophk mutants; Colour mutants; Mutagenic agents; Gamma radiation; Ultraviolet light; Nitrous acid.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161995000300023
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