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Molecular characterization of microbial contaminants isolated from Umbilical Cord Blood Units for transplant BJID
Bello-López,Juan Manuel; Noguerón-Silva,Jorge; Castañeda-Sánchez,Jorge Ismael; Rojo-Medina,Julieta.
ABSTRACT Disposal of Umbilical Cord Blood Units due to microbial contamination is a major problem in Cord Blood Banks worldwide as it reduces the number of units available for transplantation. Additionally, economic losses are generated as result of resources and infrastructure used to obtain such units. Umbilical Cord Blood Units that showed initial microbial contamination were subject to strains isolation, identification, and characterization by sequencing the 16S rRNA gene and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). Moreover, tests of antimicrobial resistance/sensitivity and phenotypic activities that may play an important role in microbial infection were performed. Microbial contamination was detected in 120 Umbilical Cord Blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Umbilical Cord Blood Units; Microbial contamination; Molecular characterization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600571
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