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Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) Genet. Mol. Biol.
Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis.
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012
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Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms Genet. Mol. Biol.
Rincón,Gonzalo; Armstrong,Eileen; Postiglioni,Alicia.
The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DGAT1 gene; Milk protein; SNPs; Uruguayan Creole cattle.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300016
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