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	Provedor de dados BJM (1) Autor Brolazo,Eliane Melo (1) Leite,Domingos Silva (1) Marconi,Camila (1) Simoes,Jose Antonio (1) Tiba,Monique Ribeiro (1) Villarroel,Marina (1) Mais... Palavra-chave Carbohydrate fermentation test (1) Identification (1) Multiplex PCR (1) Vaginal ecosystem (1) Vaginal lactobacilli (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2011 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates BJM Brolazo,Eliane Melo; Leite,Domingos Silva; Tiba,Monique Ribeiro; Villarroel,Marina; Marconi,Camila; Simoes,Jose Antonio. Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrate fermentation test; Multiplex PCR; Identification; Vaginal lactobacilli; Vaginal ecosystem. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028 Registros recuperados: 1 Primeira ... 1 ... Última

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