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| Converti,A.; Aliakbarian,B.; Domínguez,J.M.; Vázquez,G. Bustos; Perego,P.. |
| This review aims at providing an overview on the microbial production of vanillin, a new alternative method for the production of this important flavor of the food industry, which has the potential to become economically competitive in the next future. After a brief description of the applications of vanillin in different industrial sectors and of its physicochemical properties, we described the traditional ways of providing vanillin, specifically extraction and chemical synthesis (mainly oxidation) and compared them with the new biotechnological options, i.e., biotransformations of caffeic acid, veratraldehyde and mainly ferulic acid. In the second part of the review, emphasis has been addressed to the factors most influencing the bioproduction of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vanillin; Bioconversion; Review; Ferulic acid; Escherichia col. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300001 |
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| Converti,Attilio; Faveri,Danilo de; Perego,Patrizia; Barghini,Paolo; Ruzzi,Maurizio; Sene,Luciane. |
| Vanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm). Among other transformants, E. coli JM109(pBB1) appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vanillin; Ferulate; Escherichia coli JM109; Recombinant strains. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500037 |
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