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Masuda, Tsuneyuki; Kobayashi, Yoshiyasu; Maeda, Ryuichiro; Omata, Yoshitaka. |
CB-17 scid and BALB/c male mice were inoculated intraperitoneally with Neospora caninum to examine the possibility of its venereal transmission. Some of these mice were killed on days 7 and 20 post-inoculation to examine the genital organs for presence of the parasite. The remaining scid male mice were housed with non-infected female mice from day 7 p.i. and kept with them for 14 days. These scid mice died between days 28 and 35 p.i. N. caninum DNA was detected in the testis of mice on days 7 and 20 p.i. by PCR and tachyzoite viability was determined by bioassay conducted by means of mouse inoculation. Microscopically, fewer tachyzoites were detected in the testis obtained on day 20 p.i., than in other organs. The inoculated BALB/c male mice survived until... |
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Palavras-chave: CB-17 scid mouse; Neospora caninum; Venereal transmission. |
Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1657 |
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