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| Rungsa,Prapenpuksiri; Janpan,Piyapon; Saengkun,Yutthakan; Jangpromma,Nisachon; Klaynongsruang,Sompong; Patramanon,Rina; Uawonggul,Nunthawun; Daduang,Jureerut; Daduang,Sakda. |
| Abstract Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors. Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. Moreover, heterologous expression and mutagenesis of rVesA2 were performed. Results: V. affinis venom hyaluronidase full sequence is composed of 331 amino acids, with four predicted N-glycosylation sites. It was classified into the glycoside hydrolase family 56. The homology modelling exhibited a central core (α/β)7 composed of Asp107 and Glu109, acting as the catalytic residues. The recombinant protein was successfully expressed in E. coli with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vespa affinis Hyaluronidase Wasp; Venom Structure analysis Modelling Cloning Protein expression. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100321 |
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