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| Guo,Qinggang; Li,Shezeng; Lu,Xiuyun; Li,Baoqing; Ma,Ping. |
| The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Antagonism; Verticillium dahliae; Mini-Tn10. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022 |
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| Kawai, Ayako; Kusunoki, Kaori; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa; 川井, 彩子; 楠木, 芳; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久. |
| Bacillus species can form endospores and have broad-spectrum activity of their antibiotics. For that reason, they offer several advantages over other bacteria for protection against root pathogens. This work was intended to determine the ability of Bacillus spp. to inhibit Verticillium dahliae. First, antagonistic activities of Bacillus spp. were studied in greenhouse trials. These results suggest that the bacterium can control soil-borne diseases. Secondly, to investigate the situation in a culture environment, Bacillus spp. were examined for their activity to control Verticillium black spot of Japanese radish and plant-growth promotion rhizobacteria (PGPR) effects in a field. After 2 months, these bacteria reduced Verticillium black spot of Japanese... |
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Palavras-chave: Bacillus spp.; Biological control; Verticillium black spot of Japanese radish; Verticillium dahliae. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/97 |
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| 楠木, 芳; 川井, 彩子; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久; Kusunoki, Kaori; Kawai, Ayako; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa. |
| ダイコンバーティシリウム黒点病に対する防除効果を昆虫寄生菌Verticillium lecaniiを用いて検討した。温室試験と圃場試験でV. lecaniiの7菌株（A-2、B-2、C-1、2aF30、2aF31、2aF43、AaF101）を用いて実施した。V. lecaniiの分生胞子を処理したポット試験では全てのV. lecanii菌株で高い防除効果があり、病原菌単独処理と比較して有意な差が認められた。なかでもB-2が最も高い防除効果を示し、病原菌単独処理の発病率51.2％と比較して31.4％となった。V. lecaniiのフスマ培養土を用いたポット試験における病徴抑制効果は病原菌単独処理区との有意な差が認められたのはC-1処理区のみであった。A-2、B-2処理区においても抑制効果の傾向がみられたが、生長促進効果では顕著な差はみられなかった。また、圃場試験では全てのV. lecanii処理区で発病が抑制された。特にB-2、C-1においては高い発病抑制効果があり、病原菌単独処理区の半分以下に抑制していた。商品価値調査においてはV. lecanii処理区で商品価値のあるダイコンの数が優った。以上の結果から、病原菌単独処理と有意な差が認められなかったものもあるが、V. lecaniiはダイコンバーティシリウム黒点病を抑制するのに有効である傾向が確認された。よって、V. lecaniiはダイコンバーティシリウム黒点病に対して生物防除資材として有望であることが示唆された。 Seven strains of Verticillium lecanii were tested for their capacity to control Verticillium black-spot of Japanese radish... |
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Palavras-chave: ダイコンバーティシリウム黒点病; Black spot of Japanese radish; Verticillium dahliae; Verticillium lecanii. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/96 |
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| Pereira,Ricardo Borges; Resende,Mário Lúcio Vilela de; Ribeiro Júnior,Pedro Martins; Amaral,Daniel Rufino; Lucas,Gilvaine Ciavareli; Cavalcanti,Fábio Rossi. |
| O objetivo deste trabalho foi avaliar o potencial de extratos fúngicos e vegetais na redução da murcha-de-verticílio do cacaueiro, as atividades da peroxidase e polifenoloxidase e o conteúdo de lignina. Mudas de cacaueiro foram pulverizadas com filtrado de micélio de Rhizopus sp. (FMR), quitosana de Rhizopus sp. (QMR) e Trichoderma sp. (QMT), extratos de casca in natura e seca de maracujá, extrato metanólico de casca seca de frutos de maracujá (MMS) e acibenzolar-S-metil (ASM - 0.2 mg mL-1) e sete dias depois, submetidas à inoculação de Verticillium dahliae. O ASM reduziu a murcha-de-verticílio em 38,0%, seguido dos extratos FMR, QMT, MMS e QMR, que apresentaram reduções em 22,8, 20,1, 19,2 e 15,7%, respectivamente, em relação à testemunha. Plantas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Theobroma cacao; Verticillium dahliae; Lignina; Peroxidase; Polifenoloxidase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000200003 |
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| Ishida,Alessandra Keiko Nakasone; Souza,Ricardo Magela de; Resende,Mário Lúcio Vilela de; Zacaroni,Ana Beatriz; Vilas Bôas,Carla Heloísa; Souza,Jorge Teodoro de. |
| Avaliou-se o potencial de rizobactérias na indução de resistência do algodoeiro à Xanthomonas axonopodis pv. malvacearum. Após o isolamento das rizobactérias, foram selecionados os isolados capazes de reduzir os sintomas da mancha angular bacteriana em casa de vegetação, os quais foram aplicados espacialmente separados do patógeno desafiador. Os melhores isolados foram testados quanto à capacidade de reduzir os sintomas da ramulose e da murcha de Verticillium e de inibir diretamente os patógenos in vitro. Do total de 123 isolados de rizobactérias foram selecionados cinco, L2-1 (Bacillus cereus), MT5-6 (Bacillus cereus), L2-2 (Achromobacter xylosoxidans), MT5-5 (Bacillus cereus) e MT5-11 (Brevibacterium sp.), os quais apresentaram controle da mancha angular... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gossypium hirsutum; Xanthomonas axonopodis pv. malvacearum; Colletotrichum gossypii var. cephalosporioides; Verticillium dahliae; Resistência induzida. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100022 |
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