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Sowing seeds for the future: the need for establishing protocols for the study of seed dormancy 39
Silveira,Fernando Augusto Oliveira.
Seed dormancy is a widely misunderstood plant trait. In several research areas, a lack of germination is wrongly assumed to result from seed dormancy. In an attempt to standardize seed dormancy research and improve communication among seed scientists, a straightforward protocol to address the occurrence and causes of seed dormancy is provided. Standardizing communication is not just a theoretical exercise but also has practical implications in agriculture and conservation. Standardization will make studies comparable and hence we will have a deeper understanding of the physiology, ecology and evolution of seed dormancy. A better understanding of the various aspects of seed dormancy will lead to greater appreciation of the biogeographical and phylogenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dormancy classification system; Viability; Embryoless seeds; Lack of germination.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000200003
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Qualidade fisiológica de sementes de soja submetidas ao hidrocondicionamento - DOI: 10.4025/actasciagron.v30i5.5972 1
Giurizatto, Maria Izabel Kruger; Iagro; Robaina, Antonio Dias; UFGD; Gonçalves, Manoel Carlos; UFGD; Marchetti, Marlene Estevão; UFGD.
A técnica de hidratação controlada de sementes vem sendo utilizada como método de condicionamento fisiológico, tanto para sementes deterioradas como para sementes altamente sensíveis à embebição rápida, ou a interação entre ambas, objetivando melhorar o desempenho destas no campo. A hidratação pode ser seguida por secagem, se as sementes não tiverem atingido a fase III da germinação, facilitando, assim, o subseqüente manuseio, armazenamento e semeadura no campo. O objetivo do presente trabalho foi avaliar os efeitos do hidrocondicionamento na qualidade fisiológica de sementes de soja, sendo estas sementes hidrocondicionadas por períodos de 0, 20 e 24h, secas e armazenadas por 0, 90, 180 e 270 dias em câmara seca, com umidade e temperatura controladas. O...
Palavras-chave: 5.01.03.05-9 Melhoramento Vegetal Glycine max; Storage; Viability; Vigor Glycine max; Storage; Viability; Vigor.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5972
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Effects of the hydrogen potential and fungicide treatment on Pitaya seed germination 1
Ortiz, Thiago Alberto; Moritz, Aline; Oliveira, Mariana Alves de; Takahashi, Lúcia Sadayo Assari.
The objective of the present study was to evaluate pitaya seed germination under different hydrogen potentials, with or without fungicide treatment. A completely randomized design was employed under a 3 x 13 x 2 factorial scheme, corresponding to three pitaya species (white pitaya, pitaya hybrid I, pitaya hybrid II), thirteen hydrogen potentials (3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0) and two fungicide conditions (presence and absence), with four replications. The percentage of germination, germination speed index and mean germination time were evaluated. The data were subjected to ANOVA, and the means were compared using the Scott-Knott test (p < 0.05). The pH influenced the germination speed index in white pitaya. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hylocereus undatus H; Costaricensis; Carboxim+thiran; Vigor; Viability; Physiological quality Tecnologia e produção de sementes.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18088
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The germination of bush mint (Hyptis marrubioides EPL.) seeds as a function of harvest stage, light, temperature and duration of storage - doi: 10.4025/actasciagron.v33i4.4611 1
Sales, Juliana de Fátima; Instituto Federal Goiano; Pinto, José Eduardo Brasil Pereira; Universidade Federal de Lavras; Oliveira, João Almir de; Universidade Federal de Lavras; Botrel, Priscila Pereira; Universidade Federal de Lavras; Silva, Fabiano Guimarães; Instituto Federal Goiano; Corrêa, Ricardo Monteiro; Instituto Federal Minas Gerais.
This study evaluated the effect of light, temperature, physiologic stage at harvest and length of time on the germination of Hyptis marrubioides seeds. Two trials were conducted. The first experiment was performed immediately after the seed harvest and consisted of a 2 x 3 x 3 factorial design with 2 environmental conditions (light and dark), 3 temperatures (20, 30 and 20/30ºC) and 3 seed harvest times (green colored seeds, light brown seeds and dark brown seeds). The second experiment was conducted in the presence of light at a temperature of 30ºC and consisted of a 4 x 3 factorial design, with 4 storage times (0, 6, 12 and 18 months) and the 3 three harvest physiologic stages used in the previous experiment. Both of the experiments were conducted in...
Palavras-chave: 5.01.03.00-8 medicinal plant; Viability; Lamiaceae Fitotecnia medicinal plant; Viability; Lamiaceae.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4611
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An on-line advisor for sizing and economic analysis of anaerobic digestion plants 111
Busato, Patrizia; Berruto, Remigio; Sopegno, Alessandro; Cornelissen, Renè.
A web-based service was deployed to appropriately size bio-gas plant capacity and perform its economic viability and return on investment based on biomass availability.  The tool is based on accepted biogas plant engineering design practice, incorporating the effects of incentives resulting from energy policies for member nations of the European Union participating in the Bioenergy Farm IEE and EU funded project.  The service provides a comprehensive database that allows consultants and farmers to analyze anaerobic digestion systems at different levels of granularity.  It also included multilingual support.  An adoption program was conducted to increase awareness on the availability of the service and ensure appropriate use and interpretation of its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural Engineering; Information Technology Biogas; Bioenergy; Feasibility; Viability; Subsidy; Web application; Free.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2714
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Embryogenic induction of torch ginger (Etlingera elatior) callus 115
Días,Gabrielen de M. Gomes; Rodrigues,Filipe Almendagna; Soares,J. Dória Rodrigues; Pasqual,Moacir; Carvalho,A. Cristina Portugal- Pinto de.
Torch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zingiberaceae; Etlingera elatior; Viability.
Ano: 2014 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000200004
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Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids 42
DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q..
The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; DNA; Drying; RNA; Viability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217
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Cross-disciplinary approaches for measuring parasitic helminth viability and phenotype 42
Peak,Emily; Hoffmann,Karl F.
Parasitic worms (helminths) within the Phyla Nematoda and Platyhelminthes are responsible for some of the most debilitating and chronic infectious diseases of human and animal populations across the globe. As no subunit vaccine for any parasitic helminth is close to being developed, the frontline strategy for intervention is administration of therapeutic, anthelmintic drugs. Worryingly, and unsurprising due to co-evolutionary mechanisms, many of these worms are developing resistance to the limited compound classes currently being used. This unfortunate reality has led to a renaissance in next generation anthelmintic discovery within both academic and industrial sectors. However, a major bottleneck in this process is the lack of quantitative methods for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helminth; Schistosome; Nematode; Viability; Phenotype.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200024
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Relationship between number of ovipositions of Cotesia flavipes (Cam.) and number of descendants emerged from its host Diatraea saccharalis (Fabr.) 44
Yamauchi,Meire N.; Gobbi,Nivar; Chaud-Netto,José; Campos-Farinha,Ana E..
Females of Cotesia flavipes (Cam.) were individually introduced into Petri dishes containing one larva of its host Diatraea saccharalis (Fabr.). Each C. flavipes female was allowed to oviposit once or twice in the same larva. Time of emergence of larvae and adults, sex-ratio, number of non-viable larvae and pupae, and longevity of adults of C. flavipes were recorded. Data revealed that two successive ovipositions in the host did not affect sex-ratio, but increased number of parasitoids and number of non-viable larvae and pupae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Parasitoid; Superparasitism; Sex-ratio; Viability.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100012
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Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations 48
MOZDARANI,HOSSEIN; MORADI,SHABNAM Z.
This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration...
Tipo: Journal article Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004
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Intraspecific and interspecific pre-adult competition on the neotropical region colonizer Zaprionus indianus (Diptera: Drosophilidae) under laboratory conditions 53
Galego,Luís Gustavo da Conceição; Carareto,Claudia Marcia Aparecida.
This study analyzes the pre-adult interactions of Zaprionus indianus, a recently-introduced species in Brazil, with two others Drosophilidae under laboratory conditions. The effects of larval residues on the viability and on the developmental time of Z. indianus, Drosophila simulans and D. sturtevanti were used to evaluate pre-adult competitive interactions, conditioning the culture medium with larval residues. Pre-adult interactions between Z. indianus, D. sturtevanti and D. simulans may affect their relative abundance over time, since the viability of Z. indianus was negatively affected by residues of D. sturtevanti, and its residues reduced the viability of D. simulans and the developmental time of both D. simulans and D. sturtevanti.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zaprionus indianus; Fitness components; Competitive interactions; Viability; Developmental time.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000200012
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Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor 52
Komarkova,Eliska; Paca,Jan; Klapkova,Eva; Stiborova,Marie; Soccol,Carlos R; Sobotka,Miroslav.
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenol; Biodegradation; Candida tropicalis; Phenol hydroxylase; Starvation stress; Viability.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007
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Viability of bull spermatozoa collected from the epididymis stored at 18-20°C 52
Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile.
The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008
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Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability 52
Ismail,Mohd Iswadi; Osman,Khairul; Ibrahim,Siti Fatimah; Jaafar,Farah Hanan Fatihah; Ghani,Nur Azianie Abd; Zainalabidin,Fazly Ann; Othman,Abas Mazni.
The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Sperm; Canister; Motility; Viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200006
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Soy Protein Isolate-Alginate Microspheres for Encapsulation ofEnterococcus faecalis HZNU P2 52
Zhang,Yu; Zheng,Wei; Gu,Jian-Fei; Ni,Jian; Wang,Ling; Tang,Zhen-Xing; Shi,Lu-E.
ABSTRACTIn this work, the mixture of alginate and soy protein isolate used as a wall material was developed to encapsulateEnterococcus faecalis HZNU P2 (E. faecalis HZNU P2). The survival ability in the simulated gastric fluid (SGF) and bile salt solution, storage stability at different temperatures and release properties in the simulated intestinal fluid (SIF) of encapsulated cells were assessed. The results showed that encapsulation could offer sufficient protection toE. faecalis HZNU P2. The viability of encapsulatedE. faecalis HZNU P2 did not decrease in SGF at pH 2.5 or 2.0 after 2 h incubation, while free cells were reduced from 11 to 9.85 log CFU/mL in SGF (pH 2.5) at the same exposure time. Only minor viability of encapsulatedE. faecalis HZNU P2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecalis; Encapsulation; Soy protein isolate; Alginate; Stability; Viability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500805
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Effects of Storage and Exogenous Ga3 on Lychee Seed Germination 52
Ono,Elizabeth Orika; Leonel,Sarita; Duarte Filho,Jayme; Rodrigues,João Domingos.
The effects of storage time and exogenous gibberellic acid on lychee seeds germination were studied. The seeds were removed from ripe fruits, washed, dried, stored at 8°C for 0, 15 and 30 days, and soaked during 24 hours in water and GA3 at 50, 100 and 200 mg.L-1 solutions. As the storage period increased, the germination capacity was lost, and the seeds had a short germinability after 30-day storage period. Gibberellic acid had not significant effect on enhancing both the germination percentage and rate.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gibberellic acid; Gibberellins; Deterioration; Viability; Recalcitrant seed.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400015
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Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues 52
Linan,Lamia Zuniga; Lima,Nadson Murilo Nascimento; Benatti,Carol; Xavier,Mariana; Rodrigues,Ana A.; Manenti,Flavio; Jardini,Andre; Maciel Filho,Rubens; Gilioli,Rovilson.
ABSTRACT A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 µg MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PMMA; Synthesis; Cytotoxicity; Viability; Mesenchymal cells..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100601
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Effect of N-acetyl-L-cysteine on lymphocyte apoptosis, lymphocyte viability, TNF-alpha and IL-8 in HIV-infected patients undergoing anti-retroviral treatment 55
Treitinger,Aricio; Spada,Celso; Masokawa,Ivete Yoshico; Verdi,Júlio César Vidal; Silveira,Mariette Van Der  Sander; Luis,Magali Chaves; Reis,Marcellus; Ferreira,Silvia Inês Alejandra Cordova de Pires; Abdalla,Dulcinéia Saes Parra.
N-acetyl-L-cysteine (NAC) has been proposed as an additional therapeutic agent for AIDS patients because it reduces human immunodeficiency virus type 1 (HIV-1) replication in stimulated CD4+ lymphocytes, and it ameliorates immunological reactivity. In a randomized, 180-day, double-blind, placebo-controlled trial performed with HIV-infected patients classified as A2 and A3 according to the criteria of the Center for Disease Control and Prevention, we investigated the effects of oral administration of NAC on HIV-infected patients undergoing their first anti-retroviral therapy; viral load, CD4+ lymphocyte, lymphocyte viability and apoptosis, and TNF-alpha and IL-8 levels were determined. Sixteen patients who received anti-retroviral therapy plus a placebo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: N-acetylcysteine; Apoptosis; Viability; HIV; Glutathione; TNF-alpha; IL-8.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500005
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Yeast CUP1 protects HeLa cells against copper-induced stress 56
Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C..
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616
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Selective enumeration and viability of Bifidobacterium animalis subsp. lactis in a new fermented milk product 58
Antunes,Adriane Elisabete Costa; Grael,Elza Terezinha; Moreno,Izildinha; Rodrigues,Luana Gajardoni; Dourado,Fernanda Martelo; Saccaro,Daniela Marques; Lerayer,Alda Luiza Santos.
One of the key focuses of today's dairy industry worldwide is the continued development of new products, especially probiotic-based products. Buttermilk is originally a by-product of butter making fermented by Mesophilic Aromatic Cultures (MAC). It can also be made by fermentation of pasteurized whole milk or skimmed milk. This product is not marketed in Brazil. The objectives of this work were: (1) to develop a selective medium for Bifidobacterium animalis subsp. lactis enumeration and (2) to determine the viability of this microorganism during the shelf life of the buttermilk. Skim milk added with 10% sucrose or 0.03% sucralose was pasteurized and inoculated with a composite starter culture consisting of 1% MAC (containing Lactococcus lactis subsp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesophilic cultures; Buttermilk; Selectivity; Viability.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100035
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