|
|
|
Registros recuperados: 50 | |
|
|
Buglione, Celso Carlos; UFSC; Vieira, Felipe do Nascimento; UFSC; Mouriño, José Luiz Pedreira; UFSC; Pedrotti, Fabiola Santiago; UFSC; Jatoba, Adolfo; UFSC; Martins, Maurício Laterça; UFSC. |
This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7%... |
Tipo: Resistencia a infecção |
Palavras-chave: 2.12.01.02-1 Shrimp; Experimental infection; Vibrio; Virulence; Immunology bacteriologia Shrimp; Experimental infection; Vibrio; Virulence; Immunology. |
Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5471 |
| |
|
| |
|
|
Goarant, Cyrille; Merien, F. |
Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular... |
Tipo: Text |
Palavras-chave: Vibriosis; Vibrio; Real time PCR; Quantification; Mariculture; Extraction techniques. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1903.pdf |
| |
|
| |
|
|
De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre. |
Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio... |
Tipo: Text |
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf |
| |
|
| |
|
|
Luna-acosta, Andrea; Saulnier, Denis; Pommier, Mylene; Haffner, Philippe; De Decker, Sophie; Renault, Tristan; Thomas-guyon, Helene. |
Phenoloxidases (POs) are a group of copper proteins including tyrosinase, catecholase and laccase. In several insects and crustaceans, antibacterial substances are produced through the PO cascade, participating in the direct killing of invading microorganisms. However, although POs are widely recognised as an integral part of the invertebrate immune defence system, experimental evidence is lacking that these properties are conserved in molluscs, and more particularly in the Pacific oyster Crassostrea gigas. In the present study, Vibrio splendidus LGP32 and Vibrio aestuarianus 02/041 growths were affected, after being treated with C. gigas haemocyte lysate supernatant (HLS), and either a common substrate of POs, l-3,4-dihydroxyphenylalanine (l-DOPA), to... |
Tipo: Text |
Palavras-chave: Phenoloxidases; Antibacterial activity; Vibrio; Bivalve; Immunity. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00045/15598/13001.pdf |
| |
|
|
Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean. |
The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival... |
Tipo: Text |
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf |
| |
|
| |
|
|
Aguilera-rivera, Diana; Prieto-davo, Alejandra; Escalante, Karla; Chavez, Cristina; Cuzon, Gerard; Gaxiola, Gabriela. |
The advantages of FLOC over clear water (CW) in rearing juvenile L. vannamei and its effects on Vibrio communities were evaluated. Survival rate in FLOC and clear water were recorded and a probiotic was tested under both conditions. Daily growth rate (DGR) was higher in FLOC (p < 0.05) than in CW. Survival in each system increased significantly when a probiotic was included in the diet (p < 0.05). The Vibrionaceae community from the hepatopancreas (HP) and the culture medium did not differ between the two culture media. Nevertheless, a novel group of Vibrio strains was found to be unique to FLOC. No high level of lesions was observed in shrimp tissues from the FLOC + probiotic treatment; it suggests that the probiotic contributed to homeostasis and... |
Tipo: Text |
Palavras-chave: FLOC; Probiotic; L. vannamei; Disease; Vibrio. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00173/28379/26694.pdf |
| |
|
|
Rouze, Heloise; Lecellier, Gael; Saulnier, Denis; Berteaux-lecellier, Veronique. |
Coral disease outbreaks have increased over the last three decades, but their causal agents remain mostly unclear (e.g., bacteria, viruses, fungi, protists). This study details a 14-month-long survey of coral colonies in which observations of the development of disease was observed in nearly half of the sampled colonies. A bimonthly qPCR method was used to quantitatively and qualitatively evaluate Symbiodinium assemblages of tagged colonies, and to detect the presence of Vibrio spp. Firstly, our data showed that predisposition to disease development in general, and, more specifically, infection by Vibrio spp. in Acropora cytherea depended on which clades of Symbiodinium were harbored. In both cases, harboring clade D rather than A was beneficial to the... |
Tipo: Text |
Palavras-chave: Coral disease; Dynamic; Resistance; Symbiodinium assemblages; Vibrio. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00307/41848/41080.pdf |
| |
|
|
Cantet, Franck; Hervio-heath, Dominique; Caro, A; Le Mennec, Cecile; Monteil, Caroline; Quemere, Catherine; Jolivet-gougeon, Anne; Colwell, Rita R.; Monfort, Patrick. |
Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 x 10.3 MPN/l, 0.09 to 1.1 x 10.3 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were... |
Tipo: Text |
Palavras-chave: Vibrio; Lagoons; Shellfish; Water; Sediment; Human pathogen. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25890/23889.pdf |
| |
|
|
Lucas, Ronan; Courties, C.; Herbland, Alain; Goulletquer, Philippe; Marteau, Anne-laure; Lemonnier, Hugues. |
In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4... |
Tipo: Text |
Palavras-chave: Tropical shrimp aquaculture; Ecosystem shift; Phytoplankton; Bacterioplankton; Vibrio; Flow cytometry. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00029/14068/11312.pdf |
| |
|
| |
|
|
Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B. |
Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida... |
Tipo: Text |
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf |
| |
|
| |
|
|
Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank. |
The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were... |
Tipo: Text |
Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf |
| |
|
|
De Decker, Sophie; Reynaud, Yann; Saulnier, Denis. |
The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam... |
Tipo: Text |
Palavras-chave: Quorum sensing; Crassostrea gigas; Vibrio; Metalloprotease; Gene expression. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23539/21381.pdf |
| |
|
|
Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
| |
|
| |
Registros recuperados: 50 | |
|
|
|