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Registros recuperados: 10 | |
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Li, H; Parisi, M; Toubiana, Mylene; Cammarata, M; Roch, Philippe. |
The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V.... |
Tipo: Text |
Palavras-chave: Molluscs; Mytilus; ISH; Q PCR; Gene expression; Lysozyme; Heat shock; Micrococcus lysodeikticus; Vibrio anguillarum; Vibrio splendidus. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4479.pdf |
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Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L.. |
The present paper summarizes the serotyping scheme of the fish pathogenic bacterium <i>Vibrio anguillarum</i> and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O<sub>2</sub>3. However, the existence of further <i>V. anguillarum</i> strains that were not typable with any of the 23 O-antisera suggested the existence of additional... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum. |
Ano: 1999 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525 |
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Grisez, L.; Ollevier, F.P.. |
The different serotyping systems, based on thermostable O antigens, reported for <i>Vibrio anguillarum</i> and <i>V. ordalii</i> were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within <i>V. anguillarum</i> (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogens; Serology; Vibrio anguillarum. |
Ano: 1995 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970 |
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Registros recuperados: 10 | |
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