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Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais Arq. Bras. Med. Vet. Zootec.
Headley,S.A.; Santos,T.R.; Bodnar,L.; Saut,J.P.E.; Silva,A.P.; Alfieri,A.F.; Medeiros,A.A.; Soares,N.P.; Alfieri,A.A..
This study investigated the occurrence of canine distemper virus (CDV) by evaluating the presence of viral RNA within urine samples of dogs from Uberlândia, MG, with clinical manifestations suggestive of infection by CDV by targeting the CDV N gene. Of the clinical samples collected ( n =33), CDV viruria was detected in 45.5%. Five dogs died spontaneously; all had characteristic CDV-associated histopathological alterations and demonstrated CDV viruria. Statistical analyses revealed that the age, gender, breed, or the organ system of the dog affected had no influence on the occurrence of canine distemper. Myoclonus and motor incoordination were the most significant neurological manifestations observed. A direct association was observed between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine distemper; Viruria; Phylogeny; Epidemiology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601510
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BKV-infection in kidney graft dysfunction BJID
Montagner,Juliana; Michelon,Tatiana; Fontanelle,Barbara; Oliveira,Alexandre; Silveira,Janaina; Schroeder,Regina; Neumann,Jorge; Keitel,Elizete; Alexandre,Claudio Osmar Pereira.
INTRODUCTION: BKV nephropathy (BKN) causes kidney graft loss, whose specific diagnosis is invasive and might be predicted by the early detection of active viral infection. OBJECTIVE: Determine the BKV-infection prevalence in late kidney graft dysfunction by urinary decoy cell (DC) and viral DNA detection in urine (viruria) and blood (viremia; active infection). METHODS: Kidney recipients with >1 month follow-up and creatinine >1.5 mg/dL and/or recent increasing >20% (n = 120) had their urine and blood tested for BKV by semi-nested PCR, DC searching, and graft biopsy. PCR-positive patients were classified as 1+, 2+, 3+. DC, viruria and viremia prevalence, sensitivity, specificity, and likelihood ratio (LR) were determined (Table 2x2). Diagnosis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BK virus; Decoy cells; Kidney transplantation; PCR; Viremia; Viruria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200010
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