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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Angelo,P.C.S. (1) Baránek,Miroslav (1) Ferraz,M.T. (1) Goldman,G.H. (1) Goldman,M.H.S. (1) Krizan,Bretislav (1) Molfetta,J.B. (1) Pidra,Miroslav (1) Raddová,Jana (1) Silva,I. da (1) Silva,L.L.P. da (1) Mais... Palavra-chave Virus infection (2) AFLP (1) Glycine-rich protein (1) Grapevine (1) Nicotiana tabacum (1) Pistil expression (1) RNA-binding protein (1) Somaclonal variation (1) Stress (1) Stress conditions (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP Genet. Mol. Biol. Baránek,Miroslav; Raddová,Jana; Krizan,Bretislav; Pidra,Miroslav. The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Grapevine; Stress; Somaclonal variation; Virus infection; AFLP. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026 A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs BJMBR Silva,I. da; Angelo,P.C.S.; Molfetta,J.B.; Ferraz,M.T.; Silva,L.L.P. da; Goldman,G.H.; Goldman,M.H.S.. In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine-rich protein; Nicotiana tabacum; Pistil expression; RNA-binding protein; Stress conditions; Virus infection. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800001 Registros recuperados: 2 Primeira ... 1 ... Última

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