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Registros recuperados: 54 | |
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Seibert,Caroline H.; Pinto,Aguinaldo R.. |
Shrimp aquaculture has been dramatically affected by many pathogenic diseases, mainly caused by five viruses: IHHNV, YHV, TSV, WSSV, and IMNV. Here we provide a state-of-the-art overview of these shrimp viruses, with emphasis on distribution, pathology, morphology, and genomic organization, in addition to current diagnostic methods and intervention practices. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shrimp aquaculture; Viruses; Diagnostic methods; Disease control. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300002 |
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Marinho,Rebeca C.; Martins,Gabrielle R.; Souza,Kelma C.; Sousa,Ana Lídia M.; Silva,Sabrina Tainah C.; Nobre,Juliana A.; Teixeira,Maria F.S.. |
Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Standardization; Multiplex; Viruses; CAEV; MVV. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500083 |
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ANJOS, J. R. N. dos; CHARCHAR, M. J. A.; GOMES, A. C.. |
O virus do mosqueado do feijoeiro ("bean pod mottle virus" BPMV) foi identificado em plantas da soja, cultivar Itiquira, com sintomas de mosqueado, em Planaltina, Distrito Federal. Uma preparacao purificada do virus examinada em microcospio eletronico revelou a presenca de particulas isometricas em torno de 30 nm de diametro. Em testes de SDS-PAGE, foram detectadas tres proteinas com massas moleculares estimadas de 39,7, 22,9 e 21,2 kDa. A eficiencia media de transmissao do isolado do BPMV em estudo pelo crisomelideo Cerotoma arcuata Oliv. Em tres experimentos foi de 66,7%. Em experimentos de campo, o BPMV reduziu a producao de graos nas cultivares de soja Garca Branca, Garimpo, Doko, Itiquira e Pioneira em 17,1% , 17,1%, 20,4%, 20,9% e 21,4%,... |
Tipo: Folhetos |
Palavras-chave: Planaltina; Distrito Federal; Brasil; Cerotoma Arcuata; Cerrado; Glycine Max; Soja; Vírus; Brazil; Soybeans; Viruses. |
Ano: 2000 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/546792 |
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O'Carroll, Terence; Boulter, Mark; Seaver, Mary; Masson, Daniel; O'Driscoll, Michael; Roden, Cillian. |
The purpose of this course is to train and familiarise people, who are going to build, have just built or are about to modernise a purification plant, in the latest technology available to the industry and also to train people so that they can effectively run a depuration centre. ln general the course aims to : - Give participants practical experience of different systems prior to committing themselves to a particular system. - Enable participants to compare systems. - Provide a minimum training qualification to run an establishment which is essentially a facility to guarantee health and safety of products to the consumer. - Provide an overview from bacteria testing to plant lay out etc. The structure of the course is in modules, morning and... |
Tipo: Text |
Palavras-chave: Shellfish depuration; Purification; Bacteria; Viruses. |
Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00140/25082/23192.pdf |
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Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L. |
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine... |
Tipo: Text |
Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish. |
Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf |
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Elston, R. |
Serious diseases have plagued the North American oyster industry for at least 75 years. These include Malpeque Bay disease, M.S.X. disease, perkinsiosis, nocardiosis, oyster velar virus disease and others. Some of these diseases have proven intractable since they are caused by infectious agents which have not yet been transmitted in the laboratory or which cannot yet be cultivated outside of the host animal. The increased importance of aquaculture of these species and the advent of modern molecular technologies in biology have provided both the impetus and the means to now advance the science of molluscan pathology. |
Tipo: Text |
Palavras-chave: North America; Viruses; Viral diseases; Mollusc culture. |
Ano: 1989 |
URL: http://archimer.ifremer.fr/doc/1989/acte-1475.pdf |
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Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan. |
The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of... |
Tipo: Text |
Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf |
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Registros recuperados: 54 | |
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