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	Provedor de dados 5 (2) Autor Fauvel, Christian (1) Guillaume, Jean-charles (1) Jugan, P (1) Mendoza, Roberto (1) Meuzy, J (1) Zerbib, C (1) Mais... Palavra-chave Vitellogénine (2) Antibody (1) Anticorps (1) Epithelia (1) Epithelium folliculaire (1) Immuno-essai (ELISA) (1) Immunoassay (ELISA) (1) Macrobrachium rosenbergii (1) Malacostraca (1) Morphogenesis (1) Oogenesis (1) Ovaries (1) Penaeid (1) Pénéidé (1) Réseau tubulaire (1) Ultrastructure (1) Vitellin (1) Vitelline (1) Vitellogenin (1) Vitellogenèse (1) Mais... Tipo do documento Text (2) Ano 1993 (1) 1983 (1) País France (2) Idioma Francês (1) Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Homologous ELISA procedure for the determination of penaeid shrimp vitellogenin 5 Mendoza, Roberto; Guillaume, Jean-charles; Fauvel, Christian. Vitellogenin (VTG) was isolated from the haemolymph of Penaeus vannamei by a three step procedure including ultracentrifugation, gel filtration and ion exchange chromatography. VTG was used to raise polyclonal antibodies that were purified by ion exchange chromatography. A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. The inhibition curves for dilutions of haemolymph from vitellogenic females, egg yolk extracts and purified... Tipo: Text Palavras-chave: Pénéidé; Vitellogénine; Vitelline; Immuno-essai (ELISA); Anticorps; Penaeid; Vitellogenin; Vitellin; Immunoassay (ELISA); Antibody. Ano: 1993 URL: http://archimer.ifremer.fr/doc/00188/29894/28333.pdf Les cellules folliculaires secondaires de la crevette Macrobrachium rosenbergii (Decapoda, Palaemonidae): Mise en evidence d'un reseau tubulaire implique dans les echanges entre l'hemolymphe et l'ovocyte 5 Meuzy, J; Jugan, P; Zerbib, C. The epithelial cells of the ovary secondary follicles have been studied in the prawn Macrobrachium rosenbergii using electron microscope and horseradish peroxidase. A network of circomvolutioned and anastomosed tubules, measuring about 0.15 mu m in diameter, communicates with the extracellular space. This tubular network is a temporary structure which extensively increases the permeability of the follicular epithelium. Tipo: Text Palavras-chave: Macrobrachium rosenbergii; Malacostraca; Ultrastructure; Oogenesis; Morphogenesis; Epithelia; Ovaries; Vitellogénine; Réseau tubulaire; Epithelium folliculaire; Vitellogenèse; Macrobrachium rosenbergii. Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1189.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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