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Survey of pathogens in threatened wild red-tailed Amazon parrot (Amazona brasiliensis) nestlings in Rasa Island, Brazil BJM
Vaz,Frederico Fontanelli; Serafini,Patrícia Pereira; Locatelli-Dittrich,Rosangela; Meurer,Rafael; Durigon,Edison Luiz; Araújo,Jansen de; Thomazelli,Luciano Matsumiya; Ometto,Tatiana; Sipinski,Elenise Angelotti Bastos; Sezerban,Rafael Meirelles; Abbud,Maria Cecília; Raso,Tânia Freitas.
ABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian influenza virus; Chlamydia psittaci; Microbiology; Newcastle disease; West Nile virus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400747
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Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests BJM
Cargnelutti,Juliana Felipetto; Brum,Mário Celso Sperotto; Weiblen,Rudi; Flores,Eduardo Furtado.
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: West Nile virus; WNV; M/E glycoproteins; Stable expression; Diagnostic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040
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