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Gene expression of specific enological traits in wine fermentation Electron. J. Biotechnol.
Martínez,Claudio; García,Verónica; González,David; Jara,Matías; Aguilera,Matías; Ganga,Maria Angélica.
Background: Wine fermentation is a dynamic process and yeast has a precise genic regulation system that allow them to coordinate the gene transcription levels. However, the fermentation stage at which there is a great variation in the genic expression associated to a particular enological trait is often unknown and there are no simple experimental approaches to define it. Results: To identify the most adequate stage in which to evaluate the expression of the genes associated to specific enological traits we identified three stages of fermentation using the industrial strain EC1118. These stages, called early (0-16.4 hrs), middle (16.4-88.7 hrs) and late (88.7 hrs onwards), were characterized according to changes in the speed of CO2 production. The greatest...
Tipo: Journal article Palavras-chave: Enological traits; Gene expression; Saccharomyces cerevisiae; Wine fermentation; Wine yeast.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400013
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The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation Electron. J. Biotechnol.
Martínez,Claudio; Contreras,Angela; Aguilera,Omayra; Ganga,Angelica; García,Veronica.
Background Saccharomyces cerevisiae is the main microorganism responsible for alcoholic fermentation. In this process, the consumption of nitrogen is of great importance since it is found in limiting quantities and its deficiency produces sluggish and/or stuck fermentations generating large economic losses in the wine-making industry. In a previous work we compared the transcriptional profiles between genetically related strains with differences in nitrogen consumption, detecting genes with differential expression that could be associated to the differences in the levels of nitrogen consumed. One of the genes identified was ICY1. With the aim of confirming this observation, in the present work we evaluated the consumption of ammonium during the...
Tipo: Journal article Palavras-chave: Enological traits; Gene expression; Wine fermentation; Wine yeast.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400002
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