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Control of the skin scarring response Anais da ABC (AABC)
Ferreira,Lydia M.; Gragnani,Alfredo; Furtado,Fabianne; Hochman,Bernardo.
There comes a time when the understanding of the cutaneous healing process becomes essential due to the need for a precocious tissue repair to reduce the physical, social, and psychological morbidity. Advances in the knowledge on the control of interaction among cells, matrix and growth factors will provide more information on the Regenerative Medicine, an emerging area of research in medical bioengineering. However, considering the dynamism and complexity of the cutaneous healing response, it is fundamental to understand the control mechanism exerted by the interaction and synergism of both systems, cutaneous nervous and central nervous, via hypothalamus hypophysis-adrenal axis, a relevant subject, but hardly ever explored. The present study reviews the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Keratinocytes; Fibroblasts; Keloid; Tissue engineering; Skin; Plastic surgery.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300024
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Evaluation of wound healing and antimicrobial properties of aqueous extract from Bowdichia virgilioides stem barks in mice Anais da ABC (AABC)
AGRA,ISABELA K.R.; PIRES,LUANA L.S.; CARVALHO,PAULO S.M.; SILVA-FILHO,EURÍPEDES A.; SMANIOTTO,SALETE; BARRETO,EMILIANO.
The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Antimicrobial effect; Bowdichia virgilioides; Medicinal plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300945
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The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1 Anais da ABC (AABC)
DRAKULIC,DANIJELA; VICENTIC,JELENA MARJANOVIC; SCHWIRTLICH,MARIJA; TOSIC,JELENA; KRSTIC,ALEKSANDAR; KLAJN,ANDRIJANA; STEVANOVIC,MILENA.
The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; HDM2; Migration; P53; SOX2; Wound healing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100389
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Evaluation of cytotoxicity and wound healing activity of Avicennia schaueriana in cream Anais da ABC (AABC)
LOPES,CAROLINE M.I.; BARATELLA-EVÊNCIO,LIRIANE; SOUZA,IVONE A. DE; OLIVEIRA,ERWELLY B. DE; SÁ,JÉSSICA G.A.; SANTANA,MARLLON A.N.; MARCELINO NETO,PEDRO P.; SANTANA,EDUARDA S. DE; SILVA,LUZIA A. DA; VIEIRA,JEYMESSON R.C..
Abstract:Avicennia schaueriana is found in Brazilian mangrove coast. The cytotoxicity in vitro of the Aqueous Extract of Leaves of Avicennia schaueriana (AELAs) and the healing activity of the plant in cream on mice skin wounds were evaluated in this study. The cytotoxic evaluation was performed on Vero cells. The healing activity was evaluated on mice treated during 5, 10 and 15 days with cream at 5%, solution of sodium chloride at 0.9% and dexpanthenol in cream at 5%. The extract did not show cytotoxicity, but showed mitogenic activity (100μg/ml). In morphometric analysis, the percentage of wound contraction after 10 days was higher in dexpanthenol group (93.41%). In 15 days, the lowest percentage of contraction was observed in the dexpanthenol group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicennia schaueriana; In vitro techniques; Re-epithelialization; Vero cells; Wound healing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100805
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Surface, thermal and hemocompatible properties of novel single stage electrospun nanocomposites comprising polyurethane blended with bio oilTM Anais da ABC (AABC)
AYYAR,MANIKANDAN; MANI,MOHAN PRASATH; JAGANATHAN,SARAVANA KUMAR; RATHINASAMY,RAJASEKAR; KHUDZARI,AHMAD ZAHRAN; KRISHNASAMY,NAVANEETHA PANDIYARAJ.
ABSTRACT In this work, the physicochemical and blood compatibility properties of prepared PU/Bio oil nanocomposites were investigated. Scanning electron microscope (SEM) studies revealed the reduction of mean fiber diameter (709 ± 211 nm) compared to the pristine PU (969 nm ± 217 nm). Fourier transform infrared spectroscopy (FTIR) analysis exposed the characteristic peaks of pristine PU. Composite peak intensities were decreased insinuating the interaction of the bio oilTM with the PU. Contact angle analysis portrayed the hydrophobic nature of the fabricated patch compared to pristine PU. Thermal gravimetric analysis (TGA) depicted the better thermal stability of the novel nanocomposite patch and its different thermal behavior in contrast with the pristine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyurethane; Bio oilTM; Nanocomposite; Atomic force microscopy; Wound healing.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502411
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Involvement of mast cells, CD68+ and VEGF+ expressions in response to Himatanthus drasticus commercial latex in mice wound healing model Arq. Bras. Med. Vet. Zootec.
Santos,G.J.L.; Ferreira,T.C.; Rodrigues,A.L.M.; Freitas,J.C.C.; Morais,S.M.; Girão,V.C.C.; Nunes-Pinheiro,D.C.S..
ABSTRACT This study aimed to evaluate Himatanthus drasticus latex in a mice wound healing experimental model. Animals were divided into four groups (n=7) according to the treatments: GI - saline 0.9% (control), GII - mineral oil (vehicle), GIII - H. drasticus commercial latex (HdCL) and GIV - H. drasticus mixed isolated fraction (MIF, 1 mg/mL). The treatments were applied topically once daily, 50 µL for 14 consecutive days. Macroscopic lesions were evaluated, considering parameters such as swelling, redness, granulation tissue and reepithelialization. VEGF+, CD68+ expressions and mast cells (Toluidin blue stain) were evaluated. HdCL induced higher contraction and exuberant granulation tissue (P > 0.05). HdCL showed a mild inflammatory process while MIF...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Latex; Apocinaceae; Wound healing; Angiogenesis; Inflammatory infiltrate.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300513
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Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats Biol. Res.
Ahmed,Rasha R.; Mahmoud,Ayman; Ahmed,Osama M.; Metwalli,Ali; Ebaid,Hossam.
BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and...
Tipo: Journal article Palavras-chave: Diabetic model; Whey protein; Heat shock protein-72; Keratin16; Wound healing.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100054
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Co-transfection of hepatocyte growth factor and truncated TGF-β type II receptor inhibit scar formation BJMBR
Xu,Ji-Hua; Zhao,Wan-Yi; Fang,Qing-Qing; Wang,Xiao-Feng; Zhang,Ding-Ding; Hu,Yan-Yan; Zheng,Bin; Tan,Wei-Qiang.
Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signaling pathway. Hepatocyte growth factor (HGF) is a multifunctional growth factor that promotes tissue regeneration and wound healing. Theoretically, the combination of HGF and t-TGF-βRII would be expected to exert a synergistic effect on promoting wound healing and reducing collagen formation. In the present study, lentivirus-mediated transfection of the two genes (t-TGF-βRII/HGF) into fibroblasts in vitro and in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Truncated transforming growth factor-β type II receptor; Hepatocyte growth factor; Scar; Rat; Lentivirus.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100611
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The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts BJMBR
Silvestre,J.G.; Baptista,I.L.; Silva,W.J.; Cruz,A.; Silva,M.T.; Miyabara,E.H.; Labeit,S.; Moriscot,A.S..
Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fibroblast; Skeletal muscle; Migration; MuRF2; E3 ligase; Wound healing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900604
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Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits BJMBR
Chen,X.J.; Liu,S.; Gao,G.Z.; Yan,D.X.; Jiang,W.S..
This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p–control) and periosteum-lacking wounds treated with VSD (p–VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cranial bone-exposed wound; Vacuum sealing drainage; Periosteum; Wound healing; Efficacy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200602
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Effects of strain and age on ear wound healing and regeneration in mice BJMBR
Costa,R.A.; Ruiz-de-Souza,V.; Azevedo Jr.,G.M.; Vaz,N.M.; Carvalho,C.R..
Round holes in the ears of MRL mice tend to close with characteristics of regeneration believed to be absent in other mouse strains (e.g., C57BL/6). We evaluated the kinetics and the histopathology of ear wound closure in young (8 weeks old) C57BL/6 and BALB/c mice. We also used middle-aged (40 weeks old) C57BL/6 mice to evaluate the influence of aging on this process. A circular through-and-through hole was made in the ear, photographs were taken at different times after injury and wound area was measured with digital analysis software. The percentages of closed area measured on day 100 were: 23.57 ± 8.66% for young BALB/c mice, 56.47 ± 7.39% for young C57BL/6 mice, and 75.31 ± 23.65% for middle-aged C57BL/6 mice. Mice were sacrificed on days 1, 3, 5, 25,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Wound healing; Regeneration; Scar; Aging; C57BL/6 mice; BALB/c mice.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200004
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Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes BJMBR
Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M..
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602
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The inflammatory stimulus of a natural latex biomembrane improves healing in mice BJMBR
Andrade,T.A.M.; Iyer,A.; Das,P.K.; Foss,N.T.; Garcia,S.B.; Coutinho-Netto,J.; Jordão-Jr.,A.A.; Frade,M.A.C..
The aim of the present study was to compare healing obtained with biomembranes with the natural healing process (sham) using biochemical and immunohistological assays. C57BL/6 mice were divided into 4 groups of 15 mice each and received different subcutaneous implants: natural latex biomembrane (NLB), denatured latex (DL), expanded polytetrafluorethylene (ePTFE), or sham. On the 2nd, 7th, and 14th days post-treatment, 5 mice per group were sacrificed and biopsied for the following measurements: oxidative stress based on malondialdehyde (MDA), myeloperoxidase (MPO) and hydrogen peroxide by the method of ferrous oxidation-xylenol orange (FOX), as well as glutathione and total proteins; histological evaluation to enumerate inflammatory cells, fibroblasts,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Inflammation; Oxidative stress; Angiogenesis; Latex; Histomorphometry.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000009
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Moderate intensity physical training accelerates healing of full-thickness wounds in mice BJMBR
Zogaib,F.G.; Monte-Alto-Costa,A..
Physical training influences the cells and mediators involved in skin wound healing. The objective of this study was to determine the changes induced by different intensities of physical training in mouse skin wound healing. Ninety male C57BL6 mice (8 weeks old, 20-25 g) were randomized into three physical training groups: moderate (70% VO2max), high (80% VO2max), and strenuous intensity (90% VO2max). Animals trained on a motorized treadmill for 8 weeks (Elesion: physical training until the day of excisional lesion, N = 10) or 10 weeks (Eeuthan: physical training for 2 additional weeks after excisional lesion until euthanasia, N = 10), five times/week, for 45 min. Control groups (CG) trained on the treadmill three times/week only for 5 min (N = 10). In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Physical training; Wound healing; Myofibroblast; Skin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000008
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Effect of sodium carboxymethylcellulose and methylprednisolone on the healing of jejunal anastomoses in rats BJMBR
Sousa,A.A.; Petroianu,A.; Trapiello Neto,V.; Rios,V.S.; Barbosa,A.J.A..
Sodium carboxymethylcellulose (SCMC) has been effective in reducing adhesion formation and corticosteroids reduce the inflammatory process. The objective of this study was to define the intraperitoneal (ip) effects of SCMC combined with intramuscular (im) methylprednisolone on peritoneal adhesion formation and on jejunal anastomosis healing in rats. Twenty Wistar rats (200-350 g) were divided into four groups (N = 5): groups I and III (controls) 5 and 21 days of treatment before sacrifice, respectively; groups II and IV (experimental groups) 5 and 21 days of treatment, respectively. SCMC (1%) was infused into the abdominal cavity and methylprednisolone (10 mg kg-1 day-1) was injected im daily from the day before surgery for animals of groups II and IV. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bowel anastomosis; Peritoneal adhesions; Sodium carboxymethylcellulose; Corticosteroid; Histology; Wound healing.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400012
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Copaiba oil in experimental wound healing in horses Ciência Rural
Lucas,Flavia de Almeida; Kandrotas,Adriana Lima; Nardin Neto,Eugenio; Siqueira,Carlos Eduardo de; André,Gabriela Santo; Bromerschenkel,Ingrid; Perri,Silvia Helena Venturoli.
ABSTRACT: The aim of this study was to evaluate the effects of 10% copaiba oil in experimentally induced wounds in horses. Four wounds were made in the lumbar and metacarpal regions of eight adult horses. In the treatment group, the wounds received 10% copaiba oil and in the control group 0.9% sodium chloride, in the daily dressing for 21 days. The wounds were evaluated three, 7, 14, and 21 days postoperatively. No significant differences were observed between the groups. The mean lumbar wound contraction rates were 80.54% and 69.64%, for the control and treated groups, respectively. For the wounds in the metacarpal region, these averages were 44.15% and 52.48%, respectively. Under the experimental conditions of the present study, it is concluded that 10%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytotherapy; Wound healing; Equine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400601
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Immune Cells Depletion During Wound Healing as a Long-Term Effect of Undernutrition International Journal of Morphology
Zuanassi Macari,Marcelo; Misael Cavenaghi,Fabiano; Chinali Komesu,Marilena; Orive Lunardi,Laurelúcia; Sala,Miguel Angel; Novaes Júnior,Arthur Belém; Grisi,Márcio Fernando de Moraes; Taba Júnior,Mário; Scombatti de Souza,Sérgio Luiz.
Undernutrition in early life is associated with a number of acute and chronic sequelae, and recovering is a controversial issue. Even if undernutrition in Brazil is declining, studies have shown that about 31% of brazilian children still present severe or moderate malnutrition. The present study goal was to induce early malnutrition in rats and observe short- (undernourished) and long-term (after recovered) effects on defense cells involved in wound healing. Undernutrition was produced by separating the pups from the mother for 10 hours/day during the suckling period (21 days after birth). As controls were used rats at same age not submitted to suckling restriction. Undernutrition and recovering states were assessed by body weight. Skin wounds were made on...
Tipo: Journal article Palavras-chave: Undernourishment; Wound healing; Defense cells.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000100005
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Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs International Journal of Morphology
Akinrinmade,J. F; Olatunji-Akioye,A. O.
The histopathological effects of three levels of ischemia-reperfusion (IR) on healing of intestinal anastomotic wound were investigated in dogs. Three groups of dogs in which superior mesenteric artery (SMA) and collaterals were isolated but not occluded (A -control), isolated and occluded immediately distal to the aorta with collateral interruption using an atraumatic arterial clip for fifteen minutes (B) and for forty-five minutes (C) prior to ileal resection and anastomosis after return of normal perfusión in occluded groups was done. Within each group, animals were anaesthesized on third and seventh post-operative day. Grossly, impaired abdominal wound healing, intra-abdominal adhesions and partial and complete anastomotic dehiscence occurred in groups...
Tipo: Journal article Palavras-chave: Histopathology; Ischemia-reperfusion injury; Intestinal anastomoses; Wound healing.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400020
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Comparison of the Efficacy of Honey and Animal Oil in Accelerating Healing of Full Thickness Wound of mice skin International Journal of Morphology
Ghaderi,Reza; Afshar,Mohammad; Akhbarie,Hadi; Golalipour,Mohammad Jafar.
There are some medications and procedures that can be used to accelerate the healing of skin wounds. Some studies have demonstrated improvement of burn wound healing with honey treatment. In other hand, based on traditional medicine have improved wound healing with animal oil. This study was done to compare the efficacy of animal oil and honey in accelerating healing of full thickness wound of skin in mice. In this experimental study 36 male NMRI mice were subjected to full-thickness skin wounds under general anesthesia. Animals were randomly allocated to receive either single daily applications of placebo, animal oil and honey (n=12 for each group) respectively. On 4th, 7th and 10th days, four mice from each group were sacrificed using an overdose of...
Tipo: Journal article Palavras-chave: Honey; Animal oil; Wound healing; Skin; Mice.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100027
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PRP: A Possibility in Regenerative Therapy International Journal of Morphology
Issa,Joao Paulo Mardegan; Tiossi,Rodrigo; Mello,Amaro Sergio da Silva; Lopes,Ruberval Armando; Matteo,Miguel Angel Sala Di; Iyomasa,Mamie Mizusaki.
Arecent innovation in dentistry is the preparation and use of platelet-rich plasma (PRP), a concentrated suspension of the growth factors found in platelets. PRP is involved in wound healing and are postulated as promoters of tissue regeneration. This review represents an update of the uses, applications, mechanism of action and clinical benefits of PRP
Tipo: Journal article Palavras-chave: PRP; Wound healing; Dentistry.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300019
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