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Use of Inert Gases for the Preservation of Nuclear Blood Cells BABT
Khudyakov,Andrey Nikolaevich; Polezhaeva,Tatyana Vitalyevna; Zaitseva,Oksana Olegovna; Sergushkina,Marta Igorevna; Solomina,Olga Nurzadinovna.
Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clathrates; Cryopreservation; Xenon; Krypton; Leukocytes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404
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SEVOFLURANE, DESFLURANE, AND XENON NEW INHALED ANESTHETICS IN VETERINARY MEDICINE Ciência Rural
Natalini,Cláudio Correa.
Inhalation anesthesia is widely used in veterinary medicine. New inhalation anesthetics that present less untoward effects, are more potent and produce a safe and easily changeable anesthetic plane are desirable over the older agents presently available. In this review some of the physical and chemical aspects of inhalation anesthesia is revisited. Because the agents used in inhalation anesthesia are gases or vapors, the physics of vaporization, delivery and administration of these agents should be understood. The two new inhalation anesthetics sevoflurane and desflurane, and the new anesthetic gas xenon have been used in human beings for some time. In veterinary medicine there is a lack of investigation and reports that assure the safety and clinical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inhalation anesthesia; Sevoflurane; Desflurane; Xenon.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100029
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