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Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility Electron. J. Biotechnol.
Regalado,Carlos; Vázquez-Obregón,Immer; García-Almendárez,Blanca Estela; Domínguez-Domínguez,Jorge; Aguilera-Barreyro,Araceli; Amaro-Reyes,Aldo.
Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory...
Tipo: Journal article Palavras-chave: Aspergillus niger GS1; Corn stover; Digestibility; Solid-state fermentation; Xylanolytic activity.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009
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Production of xylan-degrading enzymes by a Trichoderma harzianum strain BJM
Cacais,André O.Guerreiro; Silveira,Fabiane Q. de Paula; Ferreira Filho,Edivaldo Ximenes.
Trichoderma harzianum strain 4 produced extracellular xylan-degrading enzymes, namely beta-xylanase, beta-xylosidase and alpha-arabinofuranosidase, when grown in liquid medium cultures containing oat spelt xylan as inducer. Cellulase activity was not detected. The pattern of xylan-degrading enzymes induction was influenced by the form of xylan present in the medium. They were detected in different incubation periods. Electrophoretic separation of the proteins from liquid culture filtrates by SDS-PAGE showed a variety of bands with high and low molecular weights.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Xylan; T. harzianum; Xylanolytic activity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200014
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