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Mixture of Kerosene and Xylene: a Contribution to Clearing Agents International Journal of Morphology
Ofusori,David A; Ayoka,Abiodun O; Adeeyo,Olusola A; Adewole,Stephen O.
The aim of this study was to substitute costly and hazardous compound- xylene, used as clearing agent, with less costly compounds (mixture of xylene and kerosene) having less toxicity and without compromising the cellular integrity and staining characteristics of the sections. Tissues (liver and kidney) obtained from a presumable healthy adult Wistar rat, were fixed in 10% formol saline, separated in to five groups (A, B, C, D and E) and processed for light microscopic study adopting H & E staining procedure. During the clearing section, groups A, B, C, D and E were respectively cleared in solvent 1 (xylene only), solvent 2 (70ml xylene : 30ml kerosene), solvent 3 (50ml xylene : 50ml kerosene), solvent 4 (30ml xylene : 70ml kerosene) and solvent 5...
Tipo: Journal article Palavras-chave: Xylene; Kerosene; Clearing agent; Liver; Kidney.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100036
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Effects of Xylene on Respiratory Mucosa in Rats International Journal of Morphology
Arslan,Eda; Samanci,Baver; Samanci,Bölükbasi Seyla; Özevren,Hüseyin; Deveci,E.
In this study we examined the effects histopathologic and immunohistochemical of xylene inhalation in rats by using light microscopy. Adult wistar albino rats were used in this study. Eight rats were in control group and 8 rats were in the experimental group. The experimental group was exposed to 300 ppm formaldehyde 3­5 min/day, 5 days/week for 8 weeks. The lining epithelium of respiratory mucosa showed a loss of ciliated cells with metaplasia of goblet cells, hyperplasia of squamous cells and edema, inflamation in sub epithelial area). In the group treated xylene. Disruption of cell-cell contact was observed. Weak expression of E-cadherin was observed between cells. The vascular endothelium of capillaries and venoles showed intense immunostaining for VEGF.
Tipo: Journal article Palavras-chave: Xylene; Vascular endothelial growth factor; E-Cadherin; Respiratory mucosa.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300019
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DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents Anais da ABC (AABC)
AQUINO,TATIANE DE; ZENKNER,FERNANDA F.; ELLWANGER,JOEL H.; PRÁ,DANIEL; RIEGER,ALEXANDRE.
The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technicians were sampled at two moments: Monday and Friday. DNA damage and cytotoxicity were evaluated using the Comet Assay and the Buccal Micronucleus Cytome assay. Fifteen subjects (83.5%) of the exposed group to solvents complained about some symptom probably related to contact with vapours of organic solvents. DNA damage in the exposed group to solvents was nearly 2-fold higher on Friday than on Monday, and in both moments the individuals of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA damage; Micronucleus; Toxicology; Occupational health; Organic solvents; Xylene.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100227
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