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| KASSAR,TELISSA C.; MAGALHÃES,TEREZA; S. JÚNIOR,JOSÉ V.J.; CARVALHO,AMANDA G.O.; SILVA,ANDRÉA N.M.R. DA; QUEIROZ,SABRINA R.A.; BERTANI,GIOVANI R.; GIL,LAURA H.V.G.. |
| ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gaussia luciferase; Homologous recombination in yeast; Reporter gene; Yellow fever virus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502119 |
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| The yellow fever (YF) virus is the prototype flavivirus. The use of molecular techniques has unraveled the basic mechanisms of viral genome structure and expression. Recent trends in flavivirus research include the use of infectious clone technology with which it is possible to recover virus from cloned cDNA. Using this technique, mutations can be introduced at any point of the viral genome and their resulting effect on virus phenotype can be assessed. This approach has opened new possibilities to study several biological viral features with special emphasis on the issue of virulence/attenuation of the YF virus. The feasibility of using YF virus 17D vaccine strain, for which infectious cDNA is available, as a vector for the expression of heterologous... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Flavivirus; Yellow fever virus; Attenuation; Vaccine; Gene expression. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200002 |
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