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	Provedor de dados Scientia Agricola (3) Autor Bedendo,Ivan Paulo (3) Ganem Junior,Evandro de Jesus (1) Gioria,Ricardo (1) Kitajima,Elliot Watanabe (1) Mello,Ana Paula de Oliveira Amaral (1) Munhoz,Elizeu Merizio (1) Pereira,Thays Benites Camargo (1) Ribeiro,Luiz Fernando Caldeira (1) Segnana,Luis Gonzalez (1) Mais... Palavra-chave Mollicutes (3) Yellows (3) Phloem bacteria (2) Bitter melon (1) Cucurbitaceae (1) Phytopathogenic prokaryotes (1) Sesamum indicum (L.) (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2019 (2) 2006 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Sesame phyllody associated with a 16SrI-B phytoplasma, a ‘Candidatus Phytoplasma asteris’-related strain, in Paraguay Scientia Agricola Ganem Junior,Evandro de Jesus; Segnana,Luis Gonzalez; Kitajima,Elliot Watanabe; Bedendo,Ivan Paulo. ABSTRACT: Sesame (Sesamum indicum L.) plants exhibiting symptoms of phyllody disease were observed in commercial fields in Paraguay. The symptoms were indicative of infection by phytoplasmas. Thus, the present study investigated the association between affected plants and phytoplasma, which was later analyzed using molecular and phylogenetic methods. Total DNA was extracted from symptomatic and asymptomatic plants and used in nested PCR assays using primers R16SN910601/R16SN011119 and R16F2n/16R2. Amplified products of 1.2 Kb revealed the presence of phytoplasma in all diseased plants, and electron microscopy confirmed the presence of phytoplasmas within phloem vessels. Nucleotide sequences from sesame phytoplasma shared 99 % similarity with phytoplasmas... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sesamum indicum (L.); Mollicutes; Yellows; Phloem bacteria. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019000100047 Phytoplasma associated with shoot proliferation in begonia Scientia Agricola Ribeiro,Luiz Fernando Caldeira; Mello,Ana Paula de Oliveira Amaral; Bedendo,Ivan Paulo; Gioria,Ricardo. Begonia is a very appreciated genus of ornamental plants, of economic relevancy, having species of flowers and foliage. In commercial croppings, plants exhibiting characteristic symptoms of phytoplasma infection have been observed, such as shoot proliferation, reduced plant, size small leaves and flowers, and phyllody. Leaves were sampled and total DNA was extracted to be used in nested Polymerase Chain Reaction (PCR), in order to detect and identify an expected phytoplasma. The results confirmed consistently the presence of a phytoplasma associated with symptomatic plants through the amplification of a typical genomic fragment of 1.2 kb by using the universal primers R16mF2/mR1 and R16F2n/R2. The use of specific primers R16(III)F2/R1 allowed to identify... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Yellows; Phytopathogenic prokaryotes. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500009 Molecular characterization of a phytoplasma associated with a commercial variety of Momordica charantia Scientia Agricola Munhoz,Elizeu Merizio; Pereira,Thays Benites Camargo; Bedendo,Ivan Paulo. ABSTRACT: Momordica charantia (bitter melon) presents two distinct types or varieties, known as wild type and commercial type. Plants of the wild type are hosts of a phytoplasma of the group 16SrIII-J, which is associated with a disease known as witches’ broom. However, this disease has not yet been reported in commercial bitter melon. Thus, symptomatic plants of the commercial type were analyzed in order to demonstrate the association between phytoplasmas and disease. In further assays, strains found in symptomatic plants of the commercial type were subjected to analysis of sequences of the secY gene to determine the extent of genetic diversity. Amplification of DNA fragments from genes 16Sr rRNA (1.2Kb) and secY (1.6Kb) revealed association of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Cucurbitaceae; Bitter melon; Phloem bacteria; Yellows. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001300232 Registros recuperados: 3 Primeira ... 1 ... Última

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