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Molecular cloning and expression analysis of a zebrafish novel zinc finger protein gene rnf141 Genet. Mol. Biol.
Deng,Wenqian; Sun,Huaqin; Liu,Yunqiang; Tao,Dachang; Zhang,Sizhong; Ma,Yongxin.
ZNF230 is a novel zinc finger gene cloned by our laboratory. In order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human ZNF230, named rnf141. The cDNA fragment of rnf141 was obtained by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) encodes a polypeptide of 222 amino acids which shares 75.65% identity with the human ZNF230. RT-PCR analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mRNA has a broad distribution. Zygotic rnf141 message is strongly localized in the central nervous system, as shown by whole-mount in situ hybridization. Knockdown and over expression of rnf141 can induce abnormal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rnf141; Zebrafish (Danio rerio); Development; Zinc finger protein.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300024
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Excretion Product of Shigella dysenteriae (SdyEP) Induced Cell Death in Early Larval Stage of Zebrafish (Danio rerio): Acridine Orange and Ethidium Bromide (AO/EB) in vivo Staining International Journal of Morphology
Alvarez,M; Urbina,G; Perdomo,L.
In the field of studies of acute toxicity induced by bacterial agents, Shiga toxins have been relevant due to the severity of the extra-intestinal diseases they cause. Numerous studies have shown that Shiga toxin induced apoptosis in different cell types; however, this important process has been little studied in vivo experimental models. In this study, the effects of excretion products of Shigella dysenteriae, in which Shiga toxin is present, were investigated on early larval stages of Zebrafish, an animal model with many advantages over other in vivo experimental models traditionally used. Both the collection of eggs and larvae of Zebrafish, and the product from excretion from Shigella dysenteriae (SdyEP) were performed according to laboratory standards....
Tipo: Journal article Palavras-chave: Zebrafish (Danio rerio); Shigella dysenteriae; Apoptosis; Acridine Orange; Ethidium Bromide.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100014
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Excretion Product of Shigella dysenteriae (SdyEP) Induced Cell Death in Early Larval Stage of Zebrafish (Danio rerio): Acridine Orange and Ethidium Bromide (AO/EB) in vivo Staining International Journal of Morphology
Álvarez,M; Urbina,G; Perdomo,L.
In the field of studies of acute toxicity induced by bacterial agents, Shiga toxins have been relevant due to the severity of the extra-intestinal diseases they cause. Numerous studies have shown that Shiga toxin induced apoptosis in different cell types; however, this important process has been little studied in vivo experimental models. In this study, the effects of excretion products of Shigella dysenteriae, in which Shiga toxin is present, we investigated on early larval stages of Zebrafish, an animal model with many advantages over other in vivo experimental models traditionally used. Both the collection of eggs and larvae of Zebrafish, and the product from excretion from Shigella dysenteriae (SdyEP) were performed according to laboratory standards....
Tipo: Journal article Palavras-chave: Zebrafish (Danio rerio); Shigella dysenteriae; Apoptosis; Acridine Orange; Ethidium Bromide.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400005
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