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Expression of RNA polymerase IV and V in Oryza sativa Electron. J. Biotechnol.
Moo,Leydi de Rocio Canche; González,Angela Kú; Rodríguez-Zapata,Luis Carlos; Suarez,Victor; Castaño,Enrique.
RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use...
Tipo: Journal article Palavras-chave: Oryza sativa; RNA pol IV; RNA pol V; Somatic embryogenesis; Zygotic embryogenesis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009
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Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine) Genet. Mol. Biol.
Schlögl,Paulo Sérgio; Santos,André Luis Wendt dos; Vieira,Leila do Nascimento; Floh,Eny Iochevet Segal; Guerra,Miguel Pedro.
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed development; Brazilian Pine; Embryogenesis-regulating genes; Zygotic embryogenesis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025
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