Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10.925
Primeira ... 456789101112 ... Última
Imagem não selecionada

Imprime registro no formato completo
A Review on the Celiac Trunk Compression Syndrome: Some Anatomic Clinical-Surgical Aspects International Journal of Morphology
Petrella,Selma; Prates,José Carlos.
The objective of this study was to review some celiac trunk compression syndrome aspects such as: symptom-posture relationship; absence of symptoms; syndrome-age relationship; angiographic study on anatomy of the celiac trunk stenosis; congenital or acquired origin; invasive diagnostic tests; surgical and postoperative results.
Tipo: Journal article Palavras-chave: Arterial occlusive disease; Celiac artery; Celiac plexus; Diaphragm; Syndrome.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200008
Imagem não selecionada

Imprime registro no formato completo
A Ricardian Analysis of the Impact of Climate Change on South American Farms Chilean J. Agric. Res.
Niggol Seo,S; Mendelsohn,Robert.
This study estimates the impact of climate change on South American agriculture taking into account farm adaptations. The study used a Ricardian analysis of 2300 farms to explore the effects of global warming on land values. In order to predict climate change impacts for this century, were examined climate change scenarios predicted by three Atmospheric Oceanic General Circulation Models (AOGCM): the Canadian Climate Center (CCC), the Centre for Climate System Research (CCSR), and the Parallel Climate Model (PCM) models. Several econometric specifications were tested, and five separate regressions were run: for all farms, small household farms, large commercial farms, rainfed farms, and irrigated farms. Farmland values will decrease as temperature...
Tipo: Journal article Palavras-chave: Climate change; Agriculture; Ricardian approach; South America.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100007
Imagem não selecionada

Imprime registro no formato completo
A roadmap to directed enzyme evolution and screening systems for biotechnological applications Biol. Res.
Martínez,Ronny; Schwaneberg,Ulrich.
Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution...
Tipo: Journal article Palavras-chave: Directed evolution; Enzyme engineering; High throughput screening; Random mutagenesis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400011
Imagem não selecionada

Imprime registro no formato completo
A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting Biol. Res.
Acharya,Arpan; Vaniawala,Salil; Shah,Parth; Parekh,Harsh; Misra,Rabindra Nath; Wani,Minal; Mukhopadhyaya,Pratap N.
BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p&#8201;<&#8201;0.001). Lower limit of detection of the test protocol was 50...
Tipo: Journal article Palavras-chave: HIV-1; Gag; SYBR Green; RT PCR.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100022
Imagem não selecionada

Imprime registro no formato completo
A Scanning Electron Microscopic Study on the Appendage Morphology of Astacus leptodactylus (Eschscholtz, 1823) and Pacifastacus leniusculus (Daña, 1852) (Crustácea:Decapoda: Astacoidea) International Journal of Morphology
Mustafa Harlioglu,Muzaffer.
This study compares the morphology of rostrum, pereipods 1,2,4 and mouthparts of juvenile Astacus leptodactylus with those oí Pacifastacus leniusculus. Differences in morphology were observed, in particular with regard to the mouthparts e.g. including setal armature and number of teeth on the mandible. In general, the shape of the rostra in the two species is similar in that both taper to a point with a pair of sharp spines distally. Laterally the rostrum of A. leptodactylus is bordered by a regular row of setae, which is not so well defined in P leniusculus. The observations also showed that in addition to an increase in size, changes in morphology in the feeding apparatus between the developmental stages of the two species were present. It was concluded...
Tipo: Journal article Palavras-chave: Appendages; Astacus; Crayfish; Mouthparts; Pacifastacus; Scanning electrón microscope.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400040
Imagem não selecionada

Imprime registro no formato completo
A scenario for the conversion of Common Agricultural Policy payments in Spain after the 2011 legislative proposals Ciencia e Investigación Agraria
Andrés,Silvia; García,José M; Compés,Raúl.
S. Andrés, J.M. García, and R. Compés. 2013. A scenario for the conversion of Common Agricultural Policy payments in Spain after the 2011 legislative proposals. Cien. Inv. Agr. 40(2):291-306. This paper evaluates the impact of a new model of agricultural policy outlined in legislative proposals issued by the European Commission in October 2011 and estimates its effects on agricultural aid in Spanish Autonomous Communities compared with 2009. Therefore, the OECD agricultural policy-index system was used. To the extent that transversal criteria are applied, which support the transfer of aid between territories, the results suggest that changing the payment assignation model would involve a redistribution of aid towards areas that currently least benefit from...
Tipo: Journal article Palavras-chave: Agricultural policy; CAP post-2013; Direct payments; OECD; Redistribution.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000200005
Imagem não selecionada

Imprime registro no formato completo
A Scientometric view of some Biological disciplines in Chile Biol. Res.
KRAUSKOPF,MANUEL.
During the last decade the articles published by Chilean Research Centers grew 1,73 which compares to the 2.34 fold increase of mainstream research articles registered as a whole in Latin America. However, the relative impact of the Chilean publications surpassed that of Latin America. In Biological Sciences, traditionally the strongest research area within Chile, Latin America also shows a steeper slope of growth. Qualitatively, biological disciplines in Chile are comparable to those published in Latin America although in Chile there are specialties as Physiology that surpass the average world's impact. The scientometric data is consistent with the fall in individual grants that the Chilean Research Fund (FONDECYT) has been allocating during the last...
Tipo: Journal article Palavras-chave: Research output; Biological sciences; Chile; Latin America; Scientometric analyses.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100012
Imagem não selecionada

Imprime registro no formato completo
A SEARCH FOR CORRELATIONS BETWEEN SEA SURFACE TEMPERATURES AND OPTICAL REFLECTANCES IN CHILE AND BRAZIL DERIVED FROM AVHRR/NOAA IMAGES Gayana
Gusso,Aníbal; Ducati,Jorge Ricardo; Cotlier,Carlos G; Lopez,Diego A. G.
A search is made for indicators of the presence of phytoplankton, using satellite images of the Pacific (Central Chile) and Atlantic (South Brazil). AVHRR/NOAA-16 and -17 visible (1 and 2) and thermal (4 and 5) channels were used to perform a detection test, respectively, the Suspended Particulate Matter (SPM) and the Sea Surface Temperature (SST). In Brazilian waters, a positive correlation is found between SST and SPM's reflectance. This is interpreted as due to phytoplankton being more abundant in colder waters, where nutrients availability are higher because CO2 dissolution rates, thus being a favorable environment for phytoplankton contents, which when mixed with SPM, tends to reduce the total water reflectance, since organic matter causes absorption...
Tipo: Journal article Palavras-chave: Phytoplankton biomass; Remote sensing; AVHRR/NOAA.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382004000200046
Imagem não selecionada

Imprime registro no formato completo
A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct Biol. Res.
RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011
Imagem não selecionada

Imprime registro no formato completo
A Segmental Hypoplastic Anterior Tibial Artery Coexisting with a Hypertrophied Perforating Fibular Artery a Case Report and Clinical Implications International Journal of Morphology
Ariyo,Olutayo.
Vascular injuries of the lower limb, especially from penetrating gunshot wounds, and peripheral arterial diseases are on the increase and management of these and many other lower limb injuries involve increasing usage of vascular interventions like by-pass surgery, per-cutaneous transluminal angioplasty, arterial cannulation, arterial bypass graft or minimally invasive measures like percutaneous trans-arterial catheterization, among others. A thorough knowledge of infrapopliteal branching most especially their pathways and luminal diameters are important to surgeons in selecting appropriate surgical interventions or procedures. We report the case in which one of the 3 terminal branches of the popliteal artery (PPA), the anterior tibial artery (ATA) of good...
Tipo: Journal article Palavras-chave: Segmental hypoplastic anterior tibial artery; Attenuated dorsalis pedis artery; Hypertrophied fibular artery; Infrapopliteal branching.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100002
Imagem não selecionada

Imprime registro no formato completo
A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line Biol. Res.
ARANDA,EVELYN; OWEN,GARETH I.
Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have...
Tipo: Journal article Palavras-chave: Angiogenesis; EA.hy926; HUVEC; Endothelial; Formula; In vitro assay; Matrigel.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012
Imagem não selecionada

Imprime registro no formato completo
A simple and effective pressure culture system modified from a transwell cell culture system Biol. Res.
Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin &#946;1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is...
Tipo: Journal article Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007
Imagem não selecionada

Imprime registro no formato completo
A simple and efficient method for extraction of Taq DNA polymerase Electron. J. Biotechnol.
Chen,Sique; Zheng,Xiujuan; Cao,Hongrui; Jiang,Linghui; Liu,Fangqian; Sun,Xinli.
Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activity of Taq Pol I was lost at the ammonium sulfate precipitation step, and the product contained a small amount of DNA. Results We provided a novel, simplified and low-cost method to purify the Taq Pol ? after overproduction of the enzyme in Escherichia coli, which used ethanol instead of ammonium sulfate to precipitate the enzyme. The precipitate can be directly dissolved in the storage buffer without dialysis. In addition, DNA and RNA contamination was removed with...
Tipo: Journal article Palavras-chave: Ethanol precipitation; PCR; Purification; Taq DNA polymerase.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500005
Imagem não selecionada

Imprime registro no formato completo
A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Biol. Res.
Zhang,Fanfan; Ye,Yu; Song,Deping; Guo,Nannan; Peng,Qi; Li,Anqi; Zhou,Xingrong; Chen,Yanjun; Zhang,Min; Huang,Dongyan; Tang,Yuxin.
Abstract Background Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Results In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections. The detection limit of RT-LAMP assay was 1 × 101 copies of PDCoV, which was approximately 100-fold more sensitive than gel-based one-step reverse transcription polymerase chain...
Tipo: Journal article Palavras-chave: Porcine Deltacoronavirus (PDCoV); RT-LAMP; Rapid diagnosis.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100222
Imagem não selecionada

Imprime registro no formato completo
A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages Electron. J. Biotechnol.
Kundu,Avijit; Sarkar,Debabrata; Bhattacharjee,Amit; Topdar,Niladri; Sinha,Mohit Kumar; Mahapatra,Bikash Sinha.
A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
Tipo: Journal article Palavras-chave: Corchorus; DNA extraction; Microsatellite; Mucilage; PCR; Restriction digestion.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
Imagem não selecionada

Imprime registro no formato completo
A simple method for estimating suitable territory for bioenergy species in Chile Ciencia e Investigación Agraria
Morales-Salinas,Luis; Acevedo,Edmundo; Castellaro,Giorgio; Román-Osorio,Luis; Morales-Inostroza,Jael; Alonso,Máximo F.
In the past 20 years, different areas of research concerning native and exotic species, herbaceous crops and forest plantations have been oriented toward satisfying domestic, industrial and transportation energy requirements. Because bioenergy species constitute an important resource, it would be strategic for a country to have a method for identifying areas suitable for their cultivation to properly incorporate the establishment of energy crops into land use planning. In this study, we sought to define the suitable territories for 16 bioenergy species and their energy potential based on their soil and climate requirements in Central and Southern Chile. We used an adapted version of the FAO EcoCrop database implemented through DIVA-GIS software to predict...
Tipo: Journal article Palavras-chave: Bioenergy species; EcoCrop; Ecological niche model; Land suitability.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200009
Imagem não selecionada

Imprime registro no formato completo
A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot Electron. J. Biotechnol.
Deshmukh,Vishal P; Thakare,Prashant V; Chaudhari,Uddhav S; Gawande,Prashant A.
Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA,...
Tipo: Journal article Palavras-chave: Ayurveda; DNA extraction; Medicinal plant; Terminalia species.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014
Imagem não selecionada

Imprime registro no formato completo
A simple negative selection method to identify adenovirus recombinants using colony PCR Electron. J. Biotechnol.
Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang.
Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection...
Tipo: Journal article Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008
Imagem não selecionada

Imprime registro no formato completo
A Simulation Model of Mesophytic Perennial Grasslands Chilean J. Agric. Res.
Castellaro G,Giorgio; Aguilar G,Claudio; Vera I,Raul; Morales S,Luis.
Grasslands are complex ecosystems and their processes are affected by soil, meteorological, and management variables. In this context, dynamic simulation models are useful to understand these processes and to design grassland use strategies. The aim of this study was to develop and validate a simulation model of perennial pasture growth based on soil and climate variables. A first approach considered that soil fertility levels were adequate; therefore, soil water availability and phytomass level were the main variables affecting pasture growth. The subroutines considered were water balance, pasture growth, and root biomass dynamics. The hypotheses regarding the functioning of the system were formulated as a group of equations which were solved numerically...
Tipo: Journal article Palavras-chave: Grassland simulation models; Mesophytic grasslands.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300013
Imagem não selecionada

Imprime registro no formato completo
A Single High Dose of Chlorpyrifos Reduces Long-Term Basal C-Fos Expression in the Rat Arcuate Hypothalamic Nucleus International Journal of Morphology
Carvajal,F; Lerma-Cabrera,J. M; Sánchez-Amate,M. C; Cubero,I.
Chlorpyrifos (CPF) is an organophosphate compound used worldwide as a pesticide in agriculture that, after subcutaneous injection, keeps acetylcholinesterase (AChE) activity inhibited within an organism for months. Ample clinical and experimental evidence shows that CPF exposure induces relevant and persistent neurobehavioral deficits in humans and animals, even after one single episode/injection. Additionally, clinical and epidemiological studies evidence that a significant percentage (60%) of Gulf War veterans as well as agricultural workers suffering from acute OP intoxication may have developed intolerance to nicotine and ethanol-containing beverages. Consistent with it, administration of a single high dose of CPF to adult Wistar rats elicited...
Tipo: Journal article Palavras-chave: Chlorpyrifos; Organophosphates; C-fos expression; Arcuate hypothalamic nucleus; Ethanol consumption.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100015
Registros recuperados: 10.925
Primeira ... 456789101112 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional