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| Registros recuperados: 30 | |
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| Kawai, Ayako; Kusunoki, Kaori; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa; 川井, 彩子; 楠木, 芳; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久. |
| Bacillus species can form endospores and have broad-spectrum activity of their antibiotics. For that reason, they offer several advantages over other bacteria for protection against root pathogens. This work was intended to determine the ability of Bacillus spp. to inhibit Verticillium dahliae. First, antagonistic activities of Bacillus spp. were studied in greenhouse trials. These results suggest that the bacterium can control soil-borne diseases. Secondly, to investigate the situation in a culture environment, Bacillus spp. were examined for their activity to control Verticillium black spot of Japanese radish and plant-growth promotion rhizobacteria (PGPR) effects in a field. After 2 months, these bacteria reduced Verticillium black spot of Japanese... |
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Palavras-chave: Bacillus spp.; Biological control; Verticillium black spot of Japanese radish; Verticillium dahliae. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/97 |
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| Tani, Masayuki; Koike, Masanori; Kuramochi, Katsuhisa; Higashi, Teruo; 谷, 昌幸; 小池, 正徳; 倉持, 勝久; 東, 照雄. |
| The effects of the addition of decomposing forest litter, collected from Podzolic soil organic layers (Oi, Oe and Oa) and Brown Forest soil Oe layers, on the amounts and composition of low molecular weight aliphatic carboxylic acids (LACAs) in soils were investigated by incubation experiments for 4 weeks. The total amounts of LACAs were greater at the beginning of incubation (384 to 1155 μmol kg-1), but decreased abruptly after 1 week, becoming relatively stable for a period of 1 to 4 weeks (299 to 377 μmol kg-1). The average amounts (without 0 weeks) of LACAs, especially acetic acid and citric acid, in soils incubated with forest litter under moderately moist conditions, were smaller than in the blank soil. Larger amounts of LACAs, especially formic acid,... |
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Palavras-chave: Decomposing forest litter; Forest soils; Incubation experiment; Low-molecular-weight aliphatic carboxylicacid (LACA); Soil microflora. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/98 |
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| SHINYA, Ryoji; AIUCHI, Daigo; KUSHIDA, Atsuhiko; TANI, Masayuki; KURAMOCHI, Katsuhisa; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 倉持, 勝久; 小池, 正徳. |
| The current study was conducted to investigate the pathogenicity and its mode of action by Verticillium lecanii hybrid strains in different sedentary stages of Heterodera glycines. Three different sedentary stages (pale yellow female, yellow brown cyst, and dark brown cyst) of H. glycines were treated with V. lecanii and incubated for 3 weeks on water agar. After 3 weeks of incubation, eggs were investigated for the following parameters: (i) the infection frequencies of eggs, (ii) the number of eggs laid, and (iii) the number of mature and healthy eggs. Subsequently, the fecundity of H. glycines treated with V. lecanii was investigated in greater detail. Consequently, some strains had a greater ability to infect eggs and this significantly reduced the... |
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Palavras-chave: Soybean cyst nematode; Protoplast fusion; Fecundity; Egg laying; Biological control. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2849 |
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| 小池, 正徳; 杉本, みどり; 相内, 大吾; 長尾, 英幸; 新谷, 良治; 谷, 昌幸; 倉持, 勝久. |
| Recently, Verticillium lecanii was transferred and subdivided into mainly Lecanicillium lecanii, L. muscarium, and L. longisporum according to morphological observations and PCR-RFLPs of ITS, mtDNA and β-tubulin. To clarify whether 65 isolates of V. lecanii of mainly Japanese isolates were identified as Lecanicillium spp., conidia size and the formation of secondary phialide formation were investigated under a light microscope, and DNA polymorphism in the j8-tubulin gene of V. lecanii was analyzed by PCR-RFLP. These results indicated that the examined isolates belonged to L. muscarium (60 isolates) and L. longisporum (4 isolates), except for one isolate which alone did not belong to both Lecanicillium spp. |
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Palavras-chave: Conidia size; Lecanicillium spp.; Secondaryphialide formation; Verticillium lecanii. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2534 |
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| 相内, 大吾; 馬場, ゆき子; 稲見, 圭吾; 新屋, 良治; 谷, 昌幸; 倉持, 勝久; 堀江, 早弥佳; 小池, 正徳. |
| In a previous study, protoplast fusion was performed among 3 strains of the entomopathogenic fungi Verticillium lecanii, as a result of which 174 hybrid strains were obtained. The aim of the present study was to screen desirable hybrid strains that have a wide host range or durable effects. Initially, 43 hybrid strains were used in bioassays against the cotton aphid. Of these strains, 30 induced equal or higher mortality as compared to Vertalec (41.9%). Secondly, 50 hybrid strains were used in bioassays against the greenhouse whitefly. Of these strains, 37 exhibited an equal or higher infection rate as compared to that of Mycotal (36.2%). Finally, 50 hybrid strains were applied to cucumber leaves in order to test strain viability under low humidity... |
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Palavras-chave: Biological control; Lecanicillium spp; Protoplast fusion; Screening; Verticillium lecanii. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2587 |
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| Shinya, Ryoji; Watanabe, Ai; Aiuchi, Daigo; Tani, Masayuki; Kuramochi, Katsuhisa; Kushida, Atsuhiko; Koike, Masanori; 新屋, 良治; 渡部, 愛; 相内, 大吾; 谷, 昌幸; 倉持, 勝久; 串田, 篤彦; 小池, 正徳. |
| Verticillium lecanii is a potentially useful biological control agent (BCA) for soybean cyst nematode (SCN), Heterodera glycines. The objective of this study was to screen hybrid strains of V. lecanii, derived from protoplast fusion, for effective control agents of SCN, and to investigate whether the protoplast fusion technique is an effective tool for development of nematode control agents. Three parental strains (Vertalec®, Mycotal®, and B-2) and their 162 hybrid strains were screened in greenhouse pot tests. Some of these hybrid strains suppressed damage on soybean plants and reduced the density of SCN in the soil. In particular, one hybrid strain, AaF42, was observed to reduce the nematode egg density by 93.2% as compared with the control. Furthermore,... |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4162 |
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| 小池, 正徳; 谷, 昌幸; 三浦, 秀穂; 倉持, 勝久; 佐々木, 市夫; Koike, Masanori; Tani, Masayuki; Miura, Hideho; Kuramochi, Katsuhisa; Sasaki, Ichio. |
| 実践報告 |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1761 |
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| Registros recuperados: 30 | |
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