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Registros recuperados: 9
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Comparison of growth and exploratory behavior in mice fed an exclusively milk formula diet and mice fed a food-pellet diet post weaning OAK
Ishii, Toshiaki; Ito, Takuya; Nishimura, Masakazu; 石井, 利明.
An exclusively milk formula diet stunted the growth of mice immediately following weaning. Milk-fed mice displayed a low-frequency profile of exploratory behavior, while pellet-fed mice showed high-frequency exploration. In contrast to exploratory behavior, feeding behavior did not differ significantly between milk- and pellet-fed mice. Despite showing low-frequency exploratory behavior, mice on an exclusively milk formula diet showed no difference in behavioral activities analyzed by an automatic hole-board apparatus compared to pellet-fed mice. These results suggest that the growth stunt caused by an exclusively milk formula diet retards the acquisition of active exploratory behavior without affecting the emotional state of mice.
Palavras-chave: Feeding; Exploratory behavior; Growth; Weaning; Milk formula.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/741
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Inactivation of integrin-linked kinase induces aberrant tau phosphorylation via sustained activation of glycogen synthase kinase 3 beta in N1E-115 neuroblastoma cells OAK
Ishii, Toshiaki; Furuoka, Hidefumi; Muroi, Yoshikage; Nishimura, Masakazu; 石井, 利明; 古岡, 秀文; 室井, 喜景.
Integrin-linked kinase (ILK) is a focal adhesion serine/ threonine protein kinase with an important role in integrin and growth factor signaling pathways. Recently, we demonstrated that ILK is expressed in N1E-115 neuroblastoma cells and controls integrin-dependent neurite outgrowth in serum-starved cells grown on laminin (Ishii, T., Satoh, E., and Nishimura, M. ( 2001) J. Biol. Chem. 276, 42994 - 43003). Here we report that ILK controls tau phosphorylation via regulation of glycogen synthase kinase-3beta (GSK-3beta) activity in N1E-115 cells. Stable transfection of a kinase-deficient ILK mutant (DN-ILK) resulted in aberrant tau phosphorylation in N1E-115 cells at sites recognized by the Tau-1 antibody that are identical to some of the phosphorylation...
Palavras-chave: ALZHEIMERS-DISEASE NEURITE OUTGROWTH PROTEIN; NEURONS HYPERPHOSPHORYLATION KINASE-3-BETA ACCUMULATION BINDING ROLES.
Ano: 2003 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/747
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Integrin-linked kinase controls neurite outgrowth in N1E-115 neuroblastoma cells OAK
Ishii, Toshiaki; Satoh, Eiki; Nishimura, Masakazu; 石井, 利明; 佐藤, 栄輝.
Mouse N1E-115 cells grown on a laminin matrix exhibit neurite outgrowth in response to serum deprivation. Treatment of cells with an antibody against beta (1) integrin inhibits neurite outgrowth. Thus, beta (1) integrin is involved in the neuritogenesis of N1E-115 cells on a laminin matrix. Integrin-linked kinase (ILK), a recently identified cytoplasmic serine/threonine protein kinase that binds to the cytoplasmic domain of beta (1) integrin, has an important role in transmembrane signal transduction via integrins. We report that ILK is expressed in N1E-115 cells, the expression levels of which are constant under both normal and differentiating conditions. A stable transfection of a kinase-deficient mutant of ILK (DN-ILK) results in inhibition of neurite...
Palavras-chave: NERVE GROWTH-FACTOR; ACTIVATED PROTEIN-KINASE; RHO-FAMILY GTPASES; MAP KINASE; PC12 CELLS; FACTOR RECEPTOR; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; DEPENDENT GROWTH; ADAPTER PROTEIN.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/746
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Integrin-linked kinase is involved in lactoferrin-induced anchorage-independent cell growth and survival in PC12 cells OAK
Ishii, Toshiaki; Uto, Tomoka; Mori, Kaori; Fujikawa, Ryu; 石井, 利明.
Aims: Bovine lactoferrin (bLf) causes anchorage-independent cell growth in PC12 cells. The present study investigated the mechanisms involved in bLf-induced anchorage-independent cell growth and survival in PC12 cells. Main methods: The number of adherent cells and suspended cells was estimated separately by using a methyl thiazol tetrazolium (MTT) assay, and the sum of both optical density (O.D.) (570 nm) values was used as a measure of the total number of cells. Key findings: Integrin-linked kinase (ILK) plays an important role in integrin and growth factor signaling pathways. Stable transfection of PC12 cells with a dominant negative kinase-deficient mutant of ILK (DN-ILK) inhibited bLf-induced anchorage-independent cell growth. The ILK activity in the...
Palavras-chave: Bovine lactoferrin; PC12 cell; Integrin-linked kinase; Integrin; P38 MAPK; Cell suspension; Cell adhesion; Cell growth; Cell viability; Dominant-negative mutant; Anchorage-independent growth.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2675
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Ouabain exacerbates botulinum neurotoxin-induced muscle paralysis via progression of muscle atrophy in mice OAK
Fujikawa, Ryu; Muroi, Yoshikage; Unno, Toshihiro; Ishii, Toshiaki; 室井, 喜景; 石井, 利明.
Botulinum neurotoxin serotype A (BoNT/A) inhibits acetylcholine release at the neuromuscular junction in isolated muscles, and ouabain can partially block its effect. However, it is not clear whether ouabain attenuates BoNT/A-induced neuromuscular paralysis in vivo. In this work, we investigated the effects of ouabain on BoNT/A-induced neuromuscular paralysis in mice. Ouabain was administered to mice intraperitoneally immediately after a single injection of BoNT/A into skeletal muscle. The effects of ouabain on BoNT/A-induced muscle paralysis were assessed by quantitative monitoring of muscle tension and digit abduction via the digit abduction scoring (DAS) assay. A single administration of ouabain significantly prolonged BoNT/A-induced neuromuscular...
Palavras-chave: Ouabain; Botulinum neurotoxin; Muscle atrophy; MAFbx; MuRF-1.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3123
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The mesencephalic trigeminal sensory nucleus is involved in acquisition of active exploratory behavior induced by changing from a diet of exclusively milk formula to food pellets in mice OAK
Ishii, Toshiaki; Furuoka, Hidefumi; Kitamura, Nobuo; Muroi, Yoshikage; Nishimura, Masakazu; 古岡, 秀文; 北村, 延夫; 室井, 喜景; 石井, 利明.
Post-weaning mice fed exclusively milk display low-frequency exploratory behavior [Ishii, T., Itou, T., and Nishimura, M. (2005) Life Sci. 78, 174-179] compared to mice fed a food pellet diet. This low-frequency exploratory behavior switched to high-frequency exploration after a switch from exclusively milk formula to a food pellet diet. Acquisition of the high-frequency exploratory behavior was irreversible. Recently, we demonstrated that the mesencephalic trigeminal nucleus (Me5) is involved in the control of feeding and exploratory behavior in mice without modulating the emotional state [Ishii, T., Furuoka, H., Itou, T., Kitamura, N., and Nishimura, M. (2005) Brain Res. 1048, 80-86]. We therefore investigated whether the Me5 is involved in acquisition...
Palavras-chave: Exploratory behavior; Me5 lesions; Weaning.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/739
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Volatile female odors activate the accessory olfactory system of male mice without physical contact OAK
Muroi, Yoshikage; Ishii, Toshiaki; Komori, Seiichi; Kitamura, Nobuo; Nishimura, Masakazu; 室井, 喜景; 石井, 利明; 北村, 延夫.
We previously reported that male mice are more attracted to volatile odors from intact female mice than from ovarlectornized female mice. In the present study, we investigated male attraction to volatile odors from soiled bedding collected from the cages of estrous or ovariectomized female mice. There was no difference in the total time spent sniffing volatile odors from estrous and ovariectomized female mice, suggesting that female mice emit volatile odors which are not excreted into bedding. To test this possibility, we investigated c-Fos expression in the mitral cell layer and granule cell layer of the accessory olfactory bulb 60 min after exposure of male mice to volatile odors without physical contact. Volatile odors from an estrous female mouse...
Palavras-chave: Accessory olfactory bulb; Vomeronasal organ; Pheromone; C-Fos; Habituation-dishabituation test; Sexual attraction.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/45
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インテグリンリンクドキナーゼ不活化遺伝子導入による神経原線維変化モデルの開発 OAK
石井, 利明.
平成16年度~平成18年度科学研究費補助金基盤研究(B)研究成果報告書
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1058
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神経修復時の神経突起、軸索ガイダンスに及ぼすインテグリンリンクドキナーゼの役割 OAK
石井, 利明.
平成14年度~平成15年度科学研究費補助金基盤研究(C)(2)研究成果報告書14560242
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2673
Registros recuperados: 9
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