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Registros recuperados: 32
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Biochemical and Physiological Changes in Rice Plants Due to the Application of Herbicides1 Planta Daninha
LANGARO,A.C.; AGOSTINETTO,D.; OLIVEIRA,C.; SILVA,J.D.G.; BRUNO,M.S..
ABSTRACT The application of herbicides, even if selective, can cause biochemical and physiological changes, resulting in oxidative stress. This stress comes from the accumulation of reactive oxygen species produced due to exposure to the herbicide. However, plants have developed defense strategies, which can be enzymatic or non-enzymatic. The objective of this study was to evaluate the morphological and metabolic changes such as photosynthetic parameters, oxidative damage and antioxidant enzyme activity of rice plants after applying herbicides. For this, a study was conducted in a greenhouse and laboratory and the treatments consisted of application of imazapic + imazapyr, quinclorac, bentazon, cyhalofop-butyl, penoxsulan, bispyribac-sodium and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Oxidative stress; Lipid peroxidation; Selectivity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000200277
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CARACTERIZAÇÃO MORFOANATÔMICA, CONTAGEM CROMOSSÔMICA E VIABILIDADE POLÍNICA DE BIÓTIPOS DE AZEVÉM SUSCETÍVEL E RESISTENTES AO HERBICIDA GLYPHOSATE Planta Daninha
RUCHEL,Q.; VARGAS,L.; AGOSTINETTO,D.; FERNANDO,J. A.; LÜDTKE,R.; BOBROWSKI,V. L..
RESUMOO azevém é uma planta daninha de ciclo anual, encontrada em praticamente todas as lavouras de inverno, em pomares e vinhedos da região Sul do Brasil. A espécie é normalmente controlada pelo herbicida glyphosate, no entanto, o uso continuado desse produto selecionou biótipos resistentes. Diferenças na suscetibilidade do azevém a herbicidas têm sido atribuídas a características morfológicas, anatômicas e genéticas das plantas. Os objetivos deste trabalho foram realizar a caracterização morfoanatômica, adequar metodologia para a análise citológica de cromossomos e indicar corantes para testes de viabilidade polínica de biótipos de azevém suscetível e resistentes ao herbicida glyphosate. As sementes dos biótipos de azevém suspeitos de resistência...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomia; Cromossomos; Lolium multiflorum; Morfologia; Poaceae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000300567
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CHANGE IN PHYSIOLOGICAL FEATURES IN RYEGRASS BIOTYPES IN COMPETITION WITH SOYBEAN DUE RESISTANCE TO GLYPHOSATE Planta Daninha
AGOSTINETTO,D.; OLIVEIRA,C.; LANGARO,A.C.; NOHATTO,M.A.; MANICA-BERTO,R..
ABSTRACT Herbicide resistance can cause biochemical, physiological, morphological and phenological changes in weeds, altering their competitive ability against crops. The aim of the study was to assess physiological changes and cellular damage in susceptible and resistant biotypes of ryegrass and soybeans under competition. Three experiments were conducted in a greenhouse using a completely randomized design with four replications performed in a replacement series. The ratios of soybean and to susceptible (experiment 1) and resistant ryegrass (experiment 2) and of susceptible to resistant (experiment 3) ryegrass were 100:0, 75:25, 50:50, 25:75 and 0:100. Forty days after the emergence of the soybean crop and 60 days after the emergence of the ryegrass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxidative stress; Antioxidant enzymes; Adaptability of herbicide resistant plants.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300517
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Changes in Photosynthesis and Oxidative Stress in Wheat Plants Submmited to Herbicides Application Planta Daninha
AGOSTINETTO,D.; PERBONI,L.T.; LANGARO,A.C.; GOMES,J.; FRAGA,D.S.; FRANCO,J.J..
The use of herbicides, even in tolerant crops, can cause stress evidenced by increase phytotoxicity affecting growth and development. The objectives of this study were to evaluate herbicides effect from different mechanisms of action in photosynthetic and oxidative stress parameters, as well visual phytotoxicity and wild radish control in wheat crop, cultivar Quartzo. Two trials were conducted where the first one evaluated the photosynthetic parameters on wheat plants in two seasons collection, following the application of herbicides bentazon, clodinafop, iodosulfuron, metribuzin, metsulfuron and 2,4-D; and the second one evaluated wild radish (Raphanus sativus) control, wheat phytotoxicity and yield due to bentazon, iodosulfuron, metribuzin, metsulfuron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Selectivity; ROS; Antioxidant enzymes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000100001
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COMPETITION BETWEEN WHEAT AND RYEGRASS UNDER DIFFERENT LEVELS OF NITROGEN FERTILIZATION Planta Daninha
AGOSTINETTO,D.; TAROUCO,C.P.; LANGARO,A.C.; GOMES,J.; VARGAS,L..
ABSTRACT The main limiting factors for the productive potential of wheat crops are related to the competition with weeds for resources such as water, light and nutrients. Nitrogen (N) is an important nutrient used to maintain crop productivity, as well as promoting the competitive ability of crops and therefore suppress the weed growth. In this context, the goal of this study was to evaluate the effects of wheat plants in competition with ryegrass under different levels of nitrogen fertilization. Treatments were arranged in a factorial design, where factor A consisted in nitrogen rates (0, 60, 120, 180 and 240 kg ha-1) and factor B consisted in ryegrass and wheat plants ratios, which were: 100/0 (pure stand of 64 wheat plants), 50/50 (32 wheat plants and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen; Replacement series; Triticum aestivum; Lolium multiflorum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100247
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Criteria for Decision Making and Economic Threshold Level for Wild Radish in Wheat Crop Planta Daninha
TAVARES,L.C.; LEMES,E.S.; RUCHEL,Q.; WESTENDORFF,N.R.; AGOSTINETTO,D..
ABSTRACT: Weed competition limits wheat yield by reducing the availability of essential resources for its growth and development. In this sense, this study aimed to estimate the economic threshold level (ETL) of wild radish (Raphanus raphanistrum) in competition with wheat cultivars. Treatments were arranged in a factorial scheme. The factor wheat cultivar consisted of early (BRS 328), medium (BRS 177), and late (BRS Umbu) cycles and the factor wild radish population ranged from 0 to 564 plants m-2 (10 populations) for the cultivar BRS 328, 0 to 472 plants m-2 for the cultivar BRS 177 (11 populations), and 0 to 724 plants m-2 for the cultivar BRS Umbu (10 populations). The early-cycle BRS 328 presented a higher competitive ability when compared to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum L.; Raphanus raphanistrum; Cultivars; Populations.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100204
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Does the Glyphosate Treatment Interfere Negatively on RNA Integrity in Glyphosate-Resistant and -Sensitive Conyza bonariensis? Planta Daninha
PIASECKI,C.; BENEMANN,D.; CARVALHO,I.R.; AGOSTINETTO,D.; STEWART Jr.,C.N.; VARGAS,L..
ABSTRACT: The hairy fleabane ( Conyza bonariensis (L.) Cronq.) is among the most problematic glyphosate-resistant weeds to manage around the world. In weed science, molecular approaches such as RNA sequencing (RNA-Seq) and quantitative reverse-transcription polymerase chain reaction (RT-qPCR) have been employed to study molecular responses to glyphosate treatment in Conyza species. Glyphosate treatment leads to reactive oxygen species (ROS) production in plants which could damage the RNA. Degraded RNA is an issue and can compromise further molecular analysis. Thus, the objective of this study was to evaluate whether glyphosate treatment interferes negatively on RNA integrity of glyphosate-resistant and -sensitive hairy fleabane biotypes. Two experiments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hairy fleabane; Molecular analysis; RNA integrity number (RIN).
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100175
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Evaluation of the Mechanism Responsible for the Low-Level Resistance to Glyphosate in Goosegrass Planta Daninha
ULGUIM,A.R.; FRANCO,J.J.; SILVA,J.D.G.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT Goosegrass (Eleusine indica) infests various crops, being reported as low-level resistant (LLR) to glyphosate. The objectives of this study were to evaluate the mechanisms of resistance to glyphosate in goosegrass by the cyt-P450 complex to identify changes in leaf anatomy of these plants and quantify the accumulation of shikimate in biotypes susceptible and with LLR. The metabolic rate was assessed by applying the cyt-P450 malathion inhibitor 30 minutes before the herbicide application. The analysis of the leaf anatomy consisted of a characterization of anatomic cross-sections and evaluation of the effect of glyphosate on the leaf epidermis and the conducting vessels. Plant tissue was collected 24 hours after treatment (HAT) of the dose-response...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyt-P450 monooxygenase; Shikimate; Absorption and translocation; EPSPs; Eleusine indica.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100206
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Fitness Cost and Competitive Ability of Ryegrass Susceptible and with Multiple Resistance to Glyphosate, Iodosulfuron-Methyl, and Pyroxsulam Planta Daninha
HENCKES,J.R.; CECHIN,J.; SCHMITZ,M.F.; PIASECKI,C.; VARGAS,L.; AGOSTINETTO,D..
ABSTRACT: Ryegrass is an important weed in wheat cultivations due to the evolution of resistance to different mechanisms of action. This study aimed to compare the phenological development, fitness cost, and competitive ability between ryegrass biotypes susceptible and with multiple resistance to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) and acetolactate synthase (ALS) inhibiting herbicides. Fitness cost and phenological development were evaluated from biotypes grown in pots, and morphological growth variables were determined up to 140 days after emergence. Phenological development was evaluated with a fitness cost. Competitive ability was determined in a replacement-series experiment with proportions of resistant and susceptible ryegrass. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lolium multiflorum; EPSPs; ALS; Competition.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100288
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Fitness Cost and Competitive Ability to Different Ploidy Levels in Ryegrass Genotypes Planta Daninha
SCHMITZ,M.F.; CECHIN,J.; HENCKES,J.R.; PIASECKI,C.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT: The intergenotypic competition of tetraploid ryegrass with natural diploid population may be a tool to reduce the frequency of resistant individuals in an area. This study aimed to identify and compare the phenological development, fitness cost, and competitive ability between diploid and tetraploid ryegrass genotypes. Genotypes were grown in pots, and the morphological variables of genotype growth were assessed every 15 days up to 120 days after emergence to evaluate the fitness cost. Phenological development and seed yield components were measured in a single time together with the fitness cost. Competitive ability was determined in a replacement-series experiment with proportions of tetraploid and diploid ryegrass, in which the number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lolium multiflorum; Competition; Diploid; Tetraploid; Integrated weed management.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100189
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Geographic Distribution of Ryegrass Resistent to the Clethodim Herbicide in Rio Grande do Sul1 Planta Daninha
VARGAS,L.; SCHNEIDER,T.; AGOSTINETTO,D.; BIANCHI,M.A..
ABSTRACT Ryegrass is a weed of annual cycle that is present in winter crops, in orchards and vineyards of the South region of Brazil. The species is normally controlled by the glyphosate herbicide, but the continuous use of this product caused the selection of resistant biotypes. The use of ACCase inhibitor herbicides is the main alternative for the control of this species, but it has not been satisfactory in some places, thus causing suspicion of resistance to this action mechanism. Thus, the objective of this paper was to evaluate the occurrence and geographic distribution of ryegrass biotypes that are resistant to the clethodim herbicide in the state of Rio Grande do Sul. For that, seeds of ryegrass plants that survived the application of clethodim were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lolium multiflorum; Resistance; Chemical control; Acetyl coenzyme A carboxylase.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000200365
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Glyphosate Applied at the Early Reproductive Stage Impairs Seed Production of Glyphosate-Resistant Hairy Fleabane Planta Daninha
PIASECKI,C.; MAZON,A.S.; MONGE,A.; CAVALCANTE,J.A.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT: Glyphosate-resistant hairy fleabane [Conyza bonariensis (L.) Cronq.] is one of the most important weeds in the world. Among the factors that make this weed species widely distributed in the most diverse environments is the high seed production capacity and dispersal. Hairy fleabane plants not controlled by herbicide application regrowth and overcome crop canopy, use environmental resources, interfere with crops, and complete their life cycle by producing thousands of seeds and replenishing the seed bank. Management strategies that reduce production and viability of hairy fleabane seeds can be adopted within the integrated management to reduce the seed bank and prevent further infestations. In this way, experiments were carried out in a greenhouse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conyza bonariensis; Resistance; Integrated weed management; Prevention; Soil seed bank.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100198
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Glyphosate Resistance Affect the Physiological Quality of Conyza bonariensis seeds Planta Daninha
PIASECKI,C.; MAZON,A.S.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT: High seed production and dispersal capacity and glyphosate resistance are among the main factors that have made hairy fleabane (Conyza bonariensis) one of the most important and dispersed weeds in the world. Herbicide-resistant weeds populations may have fitness cost due to molecular, physiological, and biochemical changes. This study aimed to evaluate the physiological quality of seeds of Conyza spp. and determine whether glyphosate resistance causes fitness costs for resistant biotypes. Seeds from six hairy fleabane biotypes, three glyphosate-resistant and three -sensitive, with a resistance factor average of 11.7 were studied. Among the studied biotypes, five were identified as C. bonariensis and one as C. blakei. Seed analyses were performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Herbicide resistance; Integrated weed management; Weed biology; Soil seed bank; Conyza blakei.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100157
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IDENTIFICATION AND VALIDATION OF REFERENCE GENES FOR THE NORMALIZATION IN REAL-TIME RT-QPCR ON RICE AND RED RICE IN COMPETITION, UNDER DIFFERENT NITROGEN DOSES Planta Daninha
BENEMANN,D.P.; NOHATO,A.M.; VARGAS,L.; AVILA,L.A; AGOSTINETTO,D..
ABSTRACT Real time reverse transcription polymerase chain reaction (RT-qPCR) is an important technique to analyze differences in gene expression due to its sensitivity, accuracy, and specificity. However, before analyzing the expression of the target gene, it is necessary to identify and evaluate the stability of candidate reference genes for the proper normalization. This study aimed at evaluating the stability of candidate reference genes in order to identify the most appropriate genes for the normalization of the transcription in rice and red rice in competition under different nitrogen levels, as well as to demonstrate the effectiveness of the reference gene selected for the expression of the cytosolic ascorbate peroxidase (OsAPX2). Eleven candidate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Gene expression; Normalization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100215
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Influence of Nitrogen Fertilization on Herbicide Selectivity in Rice Planta Daninha
LANGARO,A.C.; AGOSTINETTO,D.; OLIVEIRA,C.; FRANCO,J.J.; ZANDONÁ,R.R.; VARGAS,L..
ABSTRACT: Chemical weed control and nitrogen fertilization are common practices in irrigated rice crops. Nitrogen is an essential element for growth and development of plants and may influence herbicide selectivity. In this study, herbicide selectivity was evaluated according to nitrogen application time in irrigated rice crops. The experiment was conducted in the field in a split-plot design with four replications. Treatments were arranged in a factorial scheme: factor A consisted of different nitrogen application times (without nitrogen, all nitrogen applied before flooding, half of the nitrogen rate applied before flooding and the other half after flooding, all nitrogen applied after flooding); factor B consisted of the application of the herbicides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Nitrogen; Selectivity; Weeds.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100318
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Interference Periods in Soybean Crop as Affected by Emergence Times of Weeds Planta Daninha
ZANDONÁ,R.R.; AGOSTINETTO,D.; SILVA,B.M.; RUCHEL,Q.; FRAGA,D.S..
ABSTRACT: Weeds emergence times modify competition with crops. Thus, the hypothesis was that the increase in weed emergence flow decreases the period prior to interference (PPI) in soybeans and increases the critical period of interference prevention (CPIP). The objective was to determine the PPI and the CPIP of weeds in soybean crops as affected by the preferred time of weeds emergence flow. Three experiments were conducted in the field in a randomized block design with four replications. The treatments were arranged in a factorial design with factor A consisting of coexistence or weed control in soybeans and factor B for eight periods (0, 7, 14, 21, 28, 35, 42 and 135 days after crop emergence (DAE)). The numbers of emerged plants and weed dry mass by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Weed competition; Crop yield losses.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100246
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INVESTIGATION OF THE MECHANISM OF RESISTANCE TO GLYPHOSATE HERBICIDE IN HAIRY FLEABANE Planta Daninha
KASPARY,T.E.; LAMEGO,F.P.; LANGARO,A.C.; RUCHEL,Q.; AGOSTINETTO,D..
ABSTRACT The resistance of weeds to herbicides is a consequence of one or more mechanisms in the plant, responsible for not allowing the herbicide to act properly at the active site. The objective of this study was to investigate the mechanism(s) of resistance to glyphosate in Conyza bonariensis. An experiment was conducted in November and December, 2012 and repeated in February and March, 2013 using a factorial arrangement of the treatments, where the factor A corresponded to biotypes of hairy fleabane, susceptible (S) and resistant (R); the factor B to seven rates of glyphosate from 0 to 11,520 g e.a. ha-1, and factor C were simulated environmental conditions of winter (12/8 oC day/night of temperature and 10/14 h of photoperiod) and summer (28/20 oC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conyza bonariensis; Temperature; Photoperiod; EPSPs.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300555
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Longevity of Horseweed Seed Bank Depending on the Burial Depth Planta Daninha
VARGAS,A.A.M.; AGOSTINETTO,D.; ZANDONÁ,R.R.; FRAGA,D.S.; AVILA NETO,R.C..
ABSTRACT: Horseweed (Conyza spp.) is considered as one of the major weeds occurring in the agricultural areas of South America, especially in Brazil. This species stands out from the other weeds due to characteristics such as large seed production, continued seed production, dispersion over long distances and resistance to 5-enolpyruvoylshikimate 3-phosphate (EPSP) and acetolactate synthase (ALS) inhibiting herbicides, features that increase their management. Therefore, this study aimed at determining the physiological quality and longevity of horseweed seeds over 12 months. The experiment was established under field conditions, using a randomized block design with four replications. Fifty horseweed seeds were distributed into 50 g of dry soil, placed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conyza spp.; Survival; Dormancy; Germination; Viability.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100249
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METABOLIC ACTIVITY OF WHEAT AND RYEGRASS PLANTS IN COMPETITION Planta Daninha
AGOSTINETTO,D.; TAROUCO,C.P.; NOHATTO,M.A.; OLIVEIRA,C.; FRAGA,D.S..
ABSTRACT Ryegrass is an annual cycle Poacea, often considered as a weed in wheat crops. The goal of this study was to analyze oxidative stress and enzyme activity of wheat and ryegrass cultures in response to stress caused by the competition between crop and weed, in two development stages. The experiment was conducted in completely randomized design, with eight replications, in replacement series, with a population of 64 plants per pot. Treatments consisted of proportions of ryegrass and wheat plants arranged in replacement series. The tested proportions were: 100/0 (pure wheat stand), 75/25, 50/50, 25/75 and 0/100% (pure ryegrass stand). The evaluated variables were: hydrogen peroxide (H2O2) content, thiobarbituric acid-reactive species (TBARS),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Lolium multiflorum; Stress; Competitiveness.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100233
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MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD Planta Daninha
SILVA,D.R.O.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conyza bonariensis; Polymorphism; Glyphosate; PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433
Registros recuperados: 32
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