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Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for Trypanosoma congolense OAK
SUGANUMA, Keisuke; ALLAMANDA, Puttik; HAKIMI, Hassan; ZHOU, Mo; ANGELES, Jose Ma.; KAWAZU, Shin-ichiro; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well...
Palavras-chave: Animal African trypanosomosis; Drug screening; Luciferase assay; Trypanosoma congolense.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3984
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Seroprevalence of antibody to NcSAG1 antigen of Neospora caninum in cattle from Western Java, Indonesia OAK
ICHIKAWA-SEKI, Madoka; GUSWANTO, Azirwan; ALLAMANDA, Puttik; MARIAMAH, Euis Siti; WIBOWO, Putut Eko; NISHIKAWA, Yoshifumi.
Neospora caninum can cause fetal abortion and neonatal mortality in cattle, and is a cause of economic concern worldwide. This study aimed to determine the prevalence of Neospora caninum-specific antibodies in cattle from Western Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay (ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging from 0 to 87.5% in the sampled locations. The results of this study indicate latent infection rates of sampled animals were different in each location. Further studies are necessary to elucidate the relationship between N. caninum infection and abortion in cattle, and to identify risk factors...
Palavras-chave: Cattle; ELISA; Indonesia; NcSAG1; Neospora caninum.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4250
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