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Registros recuperados: 4
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Detection and discrimination of cereal and leguminous species in chestnut flour by duplex PCR Inra
Alary, R.; Buissonade, C.; Joudrier, P.; Gautier, M.F..
Chestnut and chestnut-derived products are quite expensive and thus a possible target for fraudulent labeling. To obtain a French Label of Origin, chestnut producers need to certify the purity of their products. Chestnut-derived products are consumed by people who may suffer from celiac disease or are allergic to cereals. For these reasons, we developed a qualitative PCR-based method to detect cereal and leguminous species in chestnut flour. The presence of common wheat and barley was determined by amplifying part of the puroindoline-a gene, the presence of rye by amplifying part of the secaloindoline-a gene, the presence of durum wheat, rice, maize and chickpea by amplifying part of lipid transfer protein genes, the presence of oat by amplifying part of a...
Tipo: Journal Article Palavras-chave: CHESTNUT; CEREALS; PCR; DNA; FOOD LABELING; LEGUMES.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20081cbdceff&uri=/notices/prodinra1/2008/09/
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Differential regulation of the expression of two high affinity sulfate transporters, SULTR1.1 and SULTR1.2, in Arabidopsis Inra
Rouached, H.; Wirtz, M.; Alary, R.; Hell, R.; Arpat, A.B.; Davidian, J.C.; Fourcroy, P.; Berthomieu, P..
The molecular mechanisms regulating the initial uptake of inorganic sulfate in plants are still largely unknown. The current model for the regulation of sulfate uptake and assimilation attributes positive and negative regulatory roles to O-acetyl-serine (O-acetyl-Ser) and glutathione, respectively. This model seems to suffer from exceptions and it has not yet been clearly validated whether intracellular O-acetyl-Ser and glutathione levels have impacts on regulation. The transcript level of the two high-affinity sulfate transporters SULTR1.1 and SULTR1.2 responsible for sulfate uptake from the soil solution was compared to the intracellular contents of O-acetyl-Ser, glutathione, and sulfate in roots of plants submitted to a wide diversity of experimental...
Tipo: Journal Article Palavras-chave: SULTR1.1; SULTR1.2; SULFATE; TRANSPORTER; ROOT; SPLIT ROOT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009caed0e53&uri=/notices/prodinra1/2009/03/
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Monitoring of the early molecular resistance responses of coffee (Coffea arabica L.) to the rust fungus (Hemileia vastatrix) using real-time quantitative RT-PCR Inra
Ganesh, D.; Petitot, A.S.; Silva, M.C.; Alary, R.; Lecouls, A.C.; Fernandez, D..
Molecular resistance responses of coffee (Coffea arabica L.) to the orange rust fungus Hemileia vastatrix were monitored by real-time quantitative RT-PCR analysis of gene expression. Significant activation of coffee genes by fungal infection could be observed around 12–16 h post inoculation (hpi) in the incompatible interaction. Microscopic observations indicated that, at this time, only a limited number of fungal germlings already differentiated a penetration hypha through the stomata. Activation of the CaWRKY1 gene, putatively encoding a WRKY transcription factor also occurred in the compatible interaction, but was delayed to 24 hpi. In contrast, activation of the CaR111 gene encoding a protein of unknown function only occurred in the incompatible...
Tipo: Journal Article Palavras-chave: HEMILEIA VASTATRIX.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097f7c735b&uri=/notices/prodinra1/2009/03/
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Two differentially regulated phosphate transporters from the symbiotic fungus Hebeloma cylindrosporum and phosphorus acquisition by ectomycorrhizal Pinus pinaster Inra
Tatry, M.V.; El Kassis, E.; Lambilliotte, R.; Corratgé, C.; van Aarle, I.; Amenc, L.; Alary, R.; Zimmermann, S.; Sentenac, H.; Plassard, C..
Ectomycorrhizal symbiosis markedly improves plant phosphate uptake, but the molecular mechanisms underlying this benefit are still poorly understood. We identified two ESTs in a cDNA library prepared from the ectomycorrhizal basidiomycete Hebeloma cylindrosporum with significant similarities to phosphate transporters from the endomycorrhizal fungus Glomus versiforme and from non-mycorrhizal fungi. The full-length cDNAs corresponding to these two ESTs complemented a yeast phosphate transport mutant (Dpho84). Measurements of 33P-phosphate influx into yeast expressing either cDNA demonstrated that the encoded proteins, named HcPT1 and HcPT2, were able to mediate Pi:H+ symport with different affinities for Pi (Km values of 55 and 4 lM, respectively). Real-time...
Tipo: Journal Article Palavras-chave: PHOSPHATE TRANSPORTER; HETEROLOGOUS CHARACTERIZATION; EXPRESSION; SOIL P AVAILABILITY; PHOSPHATE UPTAKE; ECTOMYCORRHIZAL SYMBIOSIS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20095ee82dc1&uri=/notices/prodinra1/2010/03/
Registros recuperados: 4
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