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Registros recuperados: 16
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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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Biosensors for the detection and monitoring of Alexandrium minutum along the French coasts ArchiMer
Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle.
The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae.
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf
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Detection and molecular identification of Pseudo-nitzschia species in natural samples from the French coasts ArchiMer
Perez Blanco, Eva; Antoine, Elisabeth; Crassous, Marie-pierre; Compere, Chantal.
Domoic acid (DA) was first detected in France in mussel flesh in 1998, outbreak that was linked to the presence of Pseudo-nitzschia pseudodelicatissima. Ever since, the phytoplankton monitoring program (REPHY, Réseau de surveillance du Phytoplancton et des Phycotoxines) also monitors DA content in shellfish flesh. Amnesic shellfish poisoning (ASP) has been recurrently detected, and was over the sanitary threshold in the years 2000 and 2004 in Brittany and in 2002 in the Mediterranean coast (Amzil, 2001; Amzil pers. comm.). Toxicity events are usually dominated by several Pseudo-nitzschia species, although different strains might be or not be toxic (Bates et al., 1998). This is why an inventory of the species present in the French coasts and their molecular...
Tipo: Text
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00089/19988/17652.pdf
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Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.
Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...
Tipo: Text Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf
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Direct detection of toxic micro-algae Alexandrium minutum through high sensitive gravimetric sensors ArchiMer
Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal.
In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of...
Tipo: Text
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf
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Etude du fouling sur la station d'essais en-milieu marin de Saint Anne du Portzic - Juin 1977 - Décembre 1977 ArchiMer
Antoine, Elisabeth.
Cette étude, commencée en Mars 1973, vise à obtenir le maximum d'informations sur les conditions de l'environnement marin de la Station d'Èssais in-situ de Sainte Anne-du-Portzic. Elle comporte deux parties : une étude des fixations animales et végétales constituant les salissures (Fasc. 1) et un suivi des paramètres physico-chimiques tels que pH, température, oxygène dissous, salinité (Fasc. 2).
Tipo: Text
Ano: 1978 URL: http://archimer.ifremer.fr/doc/00081/19177/16772.pdf
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Identification of Pseudo-nitzschia australis and P. multiseries in the Bay of Seine. Was there a relation to presence of domoic acid in king scallops in autumn 2004? ArchiMer
Nezan, Elisabeth; Antoine, Elisabeth; Fiant, Liliane; Billard, C.
In late 2004, the French phytoplankton monitoring network (REPHY of Ifremer) detected domoic acid (DA) above the EU-regulatory limit of 20 μg DA/g tissue in king scallops (Pecten maximus) from the Bay of Seine in the Channel (Fig. 1). Shellfish harvesting sites were closed for several months due to slow depuration of the scallops.
Tipo: Text
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6331.pdf
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In situ QCM DNA-biosensor probe modification ArchiMer
Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal.
We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA...
Tipo: Text Palavras-chave: QCM; DNA; Biosensor.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf
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Interaction protection cathodique et biofilm en milieu marin ArchiMer
Guezennec, Jean; Antoine, Elisabeth; Conte, Max.
In marine environments cathodic protection using sacrificial anodes or impressed current is widely used. In aerated seawater a cathodic potential of – 800 mV/SCE is generally accepted as efficient against marine corrosion. Biofilms formed on the metallic surfaces exposed to seawater can promote localized corrosion and interfere with the cathodic polarization process. Experiments were conducted both in natural environments and with a monoculture of a marine bacterium in order to understand the relationship between cathodic protection and biofilm formation. Results indicated that, in the former case, there was no evidence that biofilms significantly affect the cathodic current demand. Conversely, in sterilized seawater inoculated with a single bacterial...
Tipo: Text
Ano: 1992 URL: https://archimer.ifremer.fr/doc/00480/59137/61765.pdf
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ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...
Tipo: Text Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf
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Layer-by-Layer DNA film synthesis via branched hybridization ArchiMer
Lazerges, M.; Perrot, H.; Antoine, Elisabeth; Compere, Chantal.
A new Layer-by-Layer (LbL) DNA film synthesis, where the driving force is the natural DNA hybridization, is presented in this work. The DNA films were synthesized via a branched mechanism and do not include any chemical binders between each layer as it is the case for film obtained by other synthesis paths. Kinetics of film formation were monitored by mass measurements with an electroacoustic network analyzer. The presented synthesis is a pathway to new DNA structures which can be used in biotechnologies and is complementary to those obtained by other LbL techniques.
Tipo: Text Palavras-chave: LbL; Electroacoustic; DNA film; Multicouche; Film ADN; Électroacoustique.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6217.pdf
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New information on the ecology of deep-sea vent communities in the azores triple junction area : preliminery results of the Diva 2 cruise (May 31-July 4, 1994) ArchiMer
Desbruyeres, Daniel; Alayse, Anne-marie; Antoine, Elisabeth; Barbier, Georges; Barriga, Fjas; Biscoito, Manuel; Briand, Patrick; Brulport, Jean-pierre; Comtet, Thierry; Cornec, Laurence; Crassous, Philippe; Dando, Paul; Fabri, Marie-claire; Felbeck, Horst; Lallier, Francois; Fiala Medioni, Aline; Goncalves, Joao; Menard, Frederic; Kerdoncuff, Jacques; Patching, John; Saldanha, Luis; Sarradin, Pierre-marie.
Tipo: Text
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00179/29005/27432.pdf
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Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae) ArchiMer
Lazerges, M; Perrot, H; Antoine, Elisabeth; Defontaine, Audrey; Compere, Chantal.
We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes...
Tipo: Text Palavras-chave: DNA; Alexandrium minutum; Microbalance.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1354.pdf
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Quartz crystal microbalance, an efficient tool to design supramolecular structure on solid substrate ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Pailleret, Alain; Antoine, Elisabeth; Compere, Chantal.
Beyond interest of these step by step synthese of linear and branched DNA polymers, this work demonstrates that the quartz crystal microbalance is a sensitive tool to design supramolecular biostructures on solid substrate by following in situ thermodynamic and kinetics of successive reactions.
Tipo: Text
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00089/19997/17660.pdf
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Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy ArchiMer
Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: Text Palavras-chave: PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6830.pdf
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Toward in situ detection of algae species ArchiMer
Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal.
For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf
Registros recuperados: 16
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