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Registros recuperados: 9
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Analyse de la diversité de la tolérance et de l'accumulation de cadmium chez la laitue Inra
Zorrig, W.; Maisonneuve, B.; Davidian, J.C.; Abdelly; Berthomieu, P..
Tipo: Conference Paper Palavras-chave: CADMIUM.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201023967281&uri=/notices/prodinra1/2010/06/
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Characterization of a selenate-resistant Arabidopsis mutant. Root growth as a potential target for selenate toxicity Inra
El Kassis, E.; Cathala, N.; Rouached, H.; Fourcroy, P.; Berthomieu, P.; Terry, N.; Davidian, J.C..
Screening an Arabidopsis ( Arabidopsis thaliana) T-DNA mutant library forselenate resistance enabled us to isolate a selenate-resistant mutant line (sel1-11).Molecular and genetic characterization showed that the mutant contained a lesion in theSULTR1;2 gene that encodes a high affinity root sulfate transporter. We showed thatSULTR1; 2 is the only gene among 13 mutated genes of the Arabidopsis sulfatetransporter family whose mutation conferred selenate resistance to Arabidopsis. Theselenate resistance phenotype of the sel1-11 mutant was mirrored by an 8-fold increaseof root growth in the presence of selenate as shown by the calculated lethalconcentration values. The impairment of SULTR1; 2 activity in sel1-11 resulted in areduced 35 S-sulfate uptake...
Tipo: Journal Article Palavras-chave: SELENATE TOXICITY; SULFATE TRANSPORTER; SULFUR NUTRITION; SELENATE RESISTANCE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b053951d&uri=/notices/prodinra1/2007/09/
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Construction and characterisation of a BAC library from Arabidopsis halleri: evaluation of physical mapping based on conserved synteny with Arabidopsis thaliana Inra
Lacombe, É.; Cossegal, M.; Mirouze, M.; Adam, T.; Varoquaux, F.; Loubet, S.; Piffanelli, P.; Lebrun, M.; Berthomieu, P..
We constructed and characterized the .rst large-insert DNA BAC library for Arabidopsis halleri, a closerelative of Arabidopsis thaliana. Double size selection of high molecular weight DNA was performed toincrease the average insert size. The BAC library consists of 6128 clones of which 87% have an insert sizeabove 125 kb. Organellar DNA contamination is estimated to 1.4%. The coverage of the library isequivalent to 4.5 times the haploid genome (250 Mb), indicating the library is suitable for almost anyapplication. We explored the possibility of generating a physical map of A. halleri using the highconserved synteny existing between this species and A. thaliana. A set of unigenes separated by 50 kb in a850 kb region of A. thaliana chromosome II was used to...
Tipo: Journal Article Palavras-chave: CHROMOSOME BACTERIEN ARTIFICIEL BAC LIBRARY; PHYSICAL MAPPING; CONSERVED SYNTENY.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008ef2186b0&uri=/notices/prodinra1/2008/08/
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Differential regulation of the expression of two high affinity sulfate transporters, SULTR1.1 and SULTR1.2, in Arabidopsis Inra
Rouached, H.; Wirtz, M.; Alary, R.; Hell, R.; Arpat, A.B.; Davidian, J.C.; Fourcroy, P.; Berthomieu, P..
The molecular mechanisms regulating the initial uptake of inorganic sulfate in plants are still largely unknown. The current model for the regulation of sulfate uptake and assimilation attributes positive and negative regulatory roles to O-acetyl-serine (O-acetyl-Ser) and glutathione, respectively. This model seems to suffer from exceptions and it has not yet been clearly validated whether intracellular O-acetyl-Ser and glutathione levels have impacts on regulation. The transcript level of the two high-affinity sulfate transporters SULTR1.1 and SULTR1.2 responsible for sulfate uptake from the soil solution was compared to the intracellular contents of O-acetyl-Ser, glutathione, and sulfate in roots of plants submitted to a wide diversity of experimental...
Tipo: Journal Article Palavras-chave: SULTR1.1; SULTR1.2; SULFATE; TRANSPORTER; ROOT; SPLIT ROOT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009caed0e53&uri=/notices/prodinra1/2009/03/
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Genetic and physiological determinants controlling cadmium accumulation in lettuce (Lactuca sativa) Inra
Zorrig, W.; Sarrobert, C.; Rouached, A.; Maisonneuve, B.; Davidian, J.C.; Abdelly, C.; Berthomieu, P..
Tipo: Conference Paper Palavras-chave: CADMIUM SOIL POLLUTANT; TOXICITY; VEGETABLE; LETTUCE; CADMIUM ACCUMULATION; ROOT SYSTEM.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201087635bf6&uri=/notices/prodinra1/2010/06/
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Physiological and molecular characterization of salt response of Arabidopsis thaliana NOK2 ecotype Inra
Kaddour, R.; M'rah, S.; Karray-Bouraoui, N.; Lambert, C.; Berthomieu, P.; Lachaâl, M..
Arabidopsis thaliana is a glycophyte capable to tolerate mild salinity. Although salt sensitivity of this species, a variability of this characteristic was revealed between different ecotypes. This study presents the physiological and molecular characteristics of salt response of two ecotypes, NOK2 and Columbia (Col). Seedlings were cultivated in hydroponics in the presence of 0 or 50 mM NaCl during 25 days. Rosette leaf samples were collected after 19, 22, and 25 days for determination of physiological parameters, and after 18 days for study of DNA polymorphism. Salt treatment decreased rosette dry matter, leaf number, leaf hydration, and leaf surface area. All these effects were significantly more visible in Col than in NOK2. Moreover, the NOK2 leaves...
Tipo: Journal Article Palavras-chave: K+/Na+ SELECTIVITY; MOLECULAR MAKERS; PCR; POLYMORPHISM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105376b38d&uri=/notices/prodinra1/2010/12/
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Production of an Arabidopsis halleri foliar defensin in Escherichia coli Inra
Marquès, L.; Oomen, R.; Aumelas, A.; Le Jean, M.; Berthomieu, P..
Production of the recombinant Arabidopsis halleri defensin AhPDF1.1 in a native-like form. Mature AhPDF1.1 cDNA was cloned into pET-28-a(+) and expressed in Escherichia coli Rosetta. After a denaturing extraction, purification by metal affinity chromatography and CNBr cleavage of the His-tag, a protein without extra amino acids at the N-terminus was obtained. An oxidative folding step was then required to renature the protein that was then purified to homogeneity by a C18 HPLC separation. Mass spectroscopy and circular dichroism analyses showed that the recombinant AhPDF1.1 has the expected molecular mass and 3D-structure features of a folded defensin with four-disulfide bridges. The recombinant protein is active against the filamentous fungus Fusarium...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS HALLERI; CNBR CLEAVAGE; NATIVE-LIKE PROTEIN; PLANT DEFENSIN; RECOMBINANT AHPDF1.1.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010699df61f&uri=/notices/prodinra1/2010/12/
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Structural and functional analysis of the C-terminal STAS (sulfate transporter and anti-sigma antagonist) domain of the Arabidopsis thaliana sulfate transporter SULTR1.2 Inra
Rouached, H.; Berthomieu, P.; El Kassis, E.; Cathala, N.; Catherinot, V.; Labesse, G.; Davidian, J.C.; Fourcroy, P..
The C-terminal region of sulfate transporters from plants and animals belonging to the SLC26 family members shares a weak but significant similarity with the Bacillus sp. anti-anti-sigma protein SpoIIAA, thus defining the STAS domain (Sulfate Transporter and Anti Sigma antagonist). The present study is a structure/function analysis of the STAS domain of SULTR1.2, an Arabidopsis thaliana sulfate transporter. A 3-D model of the SULTR1.2 STAS domain was built, which indicated that it shares the SpoIIAA folds. Moreover, the phosphorylation site, which is necessary for SpoIIAA activity, is conserved in the SULTR1.2 STAS domain. The model was used to direct mutagenesis studies using a yeast mutant defective for sulfate transport. Truncation of the whole SULTR1.2...
Tipo: Journal Article Palavras-chave: HOMEOSTASIE DU FER SULTR1.2; SULFATE TRANSPORTER; BACILLUS-SUBTILIS; CHONDROCYTES.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fd48089a&uri=/notices/prodinra1/2010/11/
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Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2 Inra
Barberon, M.; Berthomieu, P.; Clairotte, M.; Shibagaki, N.; Davidian, J.C.; Gosti, F..
In Arabidopsis, SULTR1;1 and SULTR1;2 are two genes proposed to be involved inhigh-affinity sulphate uptake from the soil solution. We address here the specific issue of their functional redundancy for the uptake of sulphate and for the accumulation of its toxic analogue selenate with regard to plant growth and selenate tolerance.• Using the complete set of genotypes, including the wild-type, each one of the single sultr1;1 and sultr1;2 mutants and the resulting double sultr1;1-sultr1;2 mutant, we performed a detailed phenotypic analysis of root length, shoot biomass, sulphate uptake, sulphate and selenate accumulation and selenate tolerance.• The results all ordered the four different genotypes according to the same functional hierarchy. Wild-type and...
Tipo: Journal Article Palavras-chave: FUNCTIONAL REDUNDANCY; HIGH-AFFINITY SULPHATE; TRANSPORTER MUTANTS; SELENATE TOLERANCE; SULPHATE CONTENT; SELENATE CONTENT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200883826cd8&uri=/notices/prodinra1/2009/03/
Registros recuperados: 9
Primeira ... 1 ... Última
 

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