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Registros recuperados: 17
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A taxonomical study of benthic Prorocentrum species (Prorocentrales, Dinophyceae) from Anse Dufour (Martinique Island, eastern Caribbean Sea) ArchiMer
Chomérat, Nicolas; Bilien, Gwenael; Zentz, Frédéric.
About 30 benthic Prorocentrum species have been described, some of which producing okadaic acid and derivatives involved in diarrhetic shellfish poisoning. The western Caribbean has been extensively studied for benthic dinoflagellates associated with ciguatera, and fifteen Prorocentrum species were described from mangroves and coral reefs of Belize. In contrast, no study reported the diversity of this genus in the Eastern Caribbean, especially in the Lesser Antilles. This study adds to the biodiversity knowledge in Martinique Island by investigating one site of the Caribbean coast from 2010 to 2017. Sediment samples were collected each year in March and studied taxonomically. Identification was realized morphologically by scanning electron microscopy,...
Tipo: Text Palavras-chave: Caribbean; Dinoflagellates; LSU rDNA; Phylogeny; Prorocentrum; Taxonomy.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00469/58075/60483.pdf
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Adding new pieces to the Azadinium (Dinophyceae) diversity and biogeography puzzle: Non-toxigenic Azadinium zhuanum sp. nov. from China, toxigenic A. poporum from the Mediterranean, and a non-toxigenic A. dalianense from the French Atlantic ArchiMer
Luo, Zhaohe; Krock, Bernd; Mertens, Kenneth; Nezan, Elisabeth; Chomerat, Nicolas; Bilien, Gwenael; Tillmann, Urban; Gu, Haifeng.
The marine planktonic dinophyceaen genus Azadinium is a primary source of azaspiracids, but due to their small size its diversity may be underestimated and information on its biogeography is still limited. A new Azadinium species, A. zhuanum was obtained from the East China Sea and Yellow Sea of China by incubating surface sediments. Five strains were established by isolating single germinated cells and their morphology was examined with light microscopy and scanning electron microscopy. Azadinium zhuanum was characterized by a plate pattern of Po, cp, X, 4′, 2a, 6′′, 6C, 5S, 6′′′, 2′′′′, by a distinct ventral pore at the junction of Po, the first and fourth apical plates, and a conspicuous antapical spine. Moreover, Azadinium poporum was obtained for the...
Tipo: Text Palavras-chave: Azadinium; Azaspiracid; AZA-2; AZA-2 phosphate; Biogeography; China; French Atlantic; Mediterranean Sea.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00386/49765/51155.pdf
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Classement des zones de production conchylicole du Finistère - Etude de zone : Baie de Lanveur ArchiMer
Piclet, Guy; Monfort, Patrick; Bilien, Gwenael; Boulben, Sylviane; Le Saux, Jean-claude; Raguenes, Pierre.
La rivière de Daoulas a fait l'objet d'un classement en zone de salubrité B par arrêté préfectoral en date du 20/02/1997 pour les coquillages du groupe 3 (Crassostrea gigas). Suite à une demande d'attribution de concessions de palourdes (Tapes philiipinarum) par les professionnels, et en raison de l'absence de données colimétriques sur cette espèce, les services de la Préfecture ont sollicité l'Ifremer pour la réalisation d'une étude bactériologique spécifique. Ce document synthétise les résultats obtenus entre mars 1997 et août 1998.
Tipo: Text
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00057/16783/14236.pdf
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Classement des zones de production conchylicole du Finistère - Etude du site de la Baie de Douarnenez (29-30) ArchiMer
Piclet, Guy; Monfort, Patrick; Bilien, Gwenael; Boulben, Sylviane; Raguenes, Pierre.
Le gisement naturel d'olives (Donax trunculus) de la baie de Douarnenez a fait l'objet d'un classement en zone de salubrité B par arrêté préfectoral du 20/02/1997. Effectué sur la base d'un seul point de prélèvement (Kervel), les services de la Préfecture ont considéré comme légitime de mener une étude de zone comportant 3 points (kervel, Lestrevet et Aber plage). Les résultats obtenus viennent confirmer le classement en zone B de ces bivalves. Par ailleurs, l'analyse des données (test de Friedman) ne permet pas de mettre en évidence de différence significative de contamination fécale entre les points de prélèvements. Il est proposé, dans le cadre du réseau de contrôle microbiologique des zones de production, de réaliser une surveillance ultérieure...
Tipo: Text
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00050/16117/13599.pdf
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Contrat de rivière Odet - Steïr - Jet ArchiMer
Piclet, Guy; Monfort, Patrick; Raguenes, Pierre; Bilien, Gwenael; Boulben, Sylviane.
Tipo: Text
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00050/16122/13604.pdf
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Cryptic diversity within the harmful dinoflagellate Akashiwo sanguinea in coastal Chinese waters is related to differentiated ecological niches ArchiMer
Luo, Zhaohe; Yang, Weidong; Leaw, Chui Pin; Pospelova, Vera; Bilien, Gwenael; Liow, Guat Ru; Lim, Po Teen; Gu, Haifeng.
Blooms of the harmful dinoflagellate Akashiwo sanguinea are responsible for the mass mortality of fish and invertebrates in coastal waters. This cosmopolitan species includes several genetically differentiated clades. Four clonal cultures were established by isolating single cells from Xiamen Harbour (the East China Sea) for morphological and genetic analyses. The cultures displayed identical morphology but were genetically different, thus revealing presence of cryptic diversity in the study area. New details of the apical structure complex of Akashiwo sanguinea were also found. To investigate whether the observed cryptic diversity was related to environmental differentiation, 634 cells were obtained from seasonal water samples collected from 2008 to 2012....
Tipo: Text Palavras-chave: Biogeography; Cryptic species; Gymnodinium sanguineum; Seasonal occurrence; Speciation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00386/49771/50355.pdf
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Description and phylogenetic position of Plagiolemma distortum sp. nov., a new raphid diatom (Bacillariophyceae) from French coastal waters ArchiMer
Nezan, Elisabeth; Bilien, Gwenael; Boulben, Sylviane; Mertens, Kenneth; Chomerat, Nicolas.
A new marine raphid diatom, Plagiolemma distortum Nézan, sp. nov. is described from plankton samples from the Atlantic Ocean, south Brittany (north-western France), where it was regularly observed, but never in high abundances. Cells have an unusual and complex shape under light microscopy (LM) depending on their position and orientation. The two valves are highly vaulted and narrow in width (transapical axis). Cells contain four complexly lobed plastids and a variable number of refractive granules without particular localization. In addition, capsules containing motile cells were observed. Ultrastructural details of the frustules were investigated using scanning electron microscopy (SEM). The girdle bands are simple. Each valve possesses a sigmoid raphe...
Tipo: Text Palavras-chave: Capsules; Habitat; Molecular phylogeny; Plastids; Raphid diatom; Video.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00441/55235/56797.pdf
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Discovery of a New Clade Nested Within the Genus Alexandrium (Dinophyceae): Morpho-molecular Characterization of Centrodinium punctatum (Cleve) F.J.R. Taylor ArchiMer
Li, Zhun; Mertens, Kenneth; Nézan, Elisabeth; Chomérat, Nicolas; Bilien, Gwenael; Iwataki, Mitsunori; Shin, Hyeon Ho.
Investigation of phytoplankton from East China Sea of the Pacific Ocean, offshore Réunion Island of the Indian Ocean, and the French Atlantic coast revealed a species of poorly known armored fusiform dinoflagellate. To clarify this species, morphology and phylogeny based on mitochondrial and nuclear protein gene sequence (Cox1, Cob and Hsp90) concatenated with the SSU, ITS region and LSU rDNA sequences were analysed. Epifluorescence and scanning electron microscopy observations revealed that the nucleus of the specimen was elongated, sausage-shaped and located equatorially on the left lateral side of the cell, and that the plate formula is Po, 3′, 1a, 6″, 6C, 8S, 5‴, 1p, 2′‴. These morphological features indicate that the species can be assigned to...
Tipo: Text Palavras-chave: Fusiform dinoflagellate; Centrodinium; Alexandrium; Morphology; Phylogeny.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00483/59496/62364.pdf
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Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate ArchiMer
Nezan, Elisabeth; Siano, Raffaele; Boulben, Sylviane; Six, Christophe; Bilien, Gwenael; Cheze, Karine; Duval, Audrey; Le Panse, Sophie; Quere, Julien; Chomerat, Nicolas.
The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed...
Tipo: Text Palavras-chave: Genetic diversity; Haemolytic; Kareniaceae; Pigments; Single cell; Taxonomy.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00225/33611/32310.pdf
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Le bassin versant du Bélon : vers une restauration durable de la qualité bactériologique des eaux estuariennes ArchiMer
Monfort, Patrick; Hervio Heath, Dominique; Caprais, Marie-paule; Pommepuy, Monique; Annezo, Jean-pierre; Loaec, Solen; Le Mennec, Cecile; Guillerm, Emmanuelle; Boulben, Sylviane; Bilien, Gwenael; Bonsor, Robert; Porter, Jonathan; Pickup, Roger.
Le projet européen Cycleau, initié à l'échelle locale sur la problèmatique de l'envasement et de l'ensablement de l'estuaire du Bélon, y a également intégré un volet bactériologique en raison des contraintes fortes que faisaient peser les pics conjoncturels de contamination sur la profession conchylicole (fermetures temporaires, déclassement éventuel) mais plus largement sur l'image du territoire (impact touristique) et en définitive sur son développement durable. Pour satisfaire la restauration de la qualité des eaux estuariennes, l'IFREMER a fait appel à un certain nombre d'outils susceptibles de contribuer à la formulation d'un diagnostic pertinent et ainsi de répondre aux préoccupations des acteurs du littoral. Pour ce faire, nous avons créé un réseau...
Tipo: Text Palavras-chave: Bassin versant; Contamination fécale; Escherichia coli; Flux bactériens; Bactériophages; SIG; Évaluation.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00200/31077/29490.pdf
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Madanidinium loirii gen. et sp nov. (Dinophyceae), a new marine benthic dinoflagellate from Martinique Island, Eastern Caribbean ArchiMer
Chomerat, Nicolas; Bilien, Gwenael.
A new benthic phototrophic dinoflagellate is described from sediments of a tropical marine cove at Martinique Island and its micromorphology is studied by means of light and electron microscopy. The cell contains small golden-brown chloroplasts and the oval nucleus is posterior. It is laterally compressed, almost circular in shape when viewed laterally. It consists of a small epitheca tilted toward the right lateral side and a larger hypotheca. In the left view, the cingulum is more anterior and the epitheca is reduced. The cingulum is displaced and left-handed. This organism is peculiar in having no apical pore and its thecal plate arrangement is 2 ' 1a 7 '' 5c 3s 5 ''' 1 ''''. The plates are smooth with small groups of pores scattered on their surface....
Tipo: Text Palavras-chave: Caribbean; Benthic; Dinophyceae; SEM; Phylogeny; Morphology; Taxonomy; Martinique; RDNA.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00195/30622/29458.pdf
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Morphological and Molecular Characterisation of Three New Azadinium Species (Amphidomataceae, Dinophyceae) from the Irminger Sea ArchiMer
Tillmann, Urban; Gottschling, Marc; Nezan, Elisabeth; Krock, Bernd; Bilien, Gwenael.
Some species of the planktonic dinoflagellate genus Azadinium produce azaspiracids (AZAs), a group of lipophilic phycotoxins causing human poisoning after mussel consumption. We describe three new species from the North Atlantic, all of which shared the same Kofoidean plate pattern characteristic for Azadinium: Po, cp, X, 4′, 3a, 6′́, 6 C, 5S, 6′́́, 2′́́́. Azadinium trinitatum sp. nov. was mainly characterized by the presence of an antapical spine and by the position of the ventral pore at the left distal end of the pore plate in a cavity of Plate 1′ Plate 1. Azadinium cuneatum sp. nov. had a conspicuously formed first apical plate, which was asymmetrically elongated and tapered on its left lateral side with a ventral pore located at the tip of this...
Tipo: Text Palavras-chave: Azadinium; Azaspiracids; Irminger Sea; Iceland; New species.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29917/28382.pdf
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Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation ArchiMer
Garvette, Andrea; Nezan, Elisabeth; Badis, Yacine; Bilien, Gwenael; Arce, Paola; Bresnan, Eileen; Gachon, Claire M. M.; Siano, Raffaele.
Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus...
Tipo: Text Palavras-chave: Pseudo-nitzschia; Diatoms; Marine oomycetes; Plankton parasites; Single-cell analysis; Metabarcoding.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00470/58135/60593.pdf
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Ostreopsis lenticularis Y. Fukuyo (Dinophyceae, Gonyaulacales) from French Polynesia (South Pacific Ocean): A revisit of its morphology, molecular phylogeny and toxicity ArchiMer
Chomérat, Nicolas; Bilien, Gwenael; Derrien, Amelie; Henry, Kévin; Ung, André; Viallon, Jérôme; Darius, Hélène Taiana; Mahana Iti Gatti, Clémence; Roué, Mélanie; Hervé, Fabienne; Réveillon, Damien; Amzil, Zouher; Chinain, Mireille.
To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French...
Tipo: Text Palavras-chave: ITS-5.8S rDNA; LSU rDNA; Microscopy; Ostreopsis lenticularis; Taxonomy; Toxins.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59747/62887.pdf
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Pentaplacodinium saltonense gen. et sp. nov. (Dinophyceae) and its relationship to the cyst-defined genus Operculodinium and yessotoxin-producing Protoceratium reticulatum ArchiMer
Mertens, Kenneth; Carbonell-moore, Maria Consuelo; Pospeloya, Vera; Head, Martin J.; Highfield, Andrea; Schroeder, Declan; Gu, Haifeng; Andree, Karl B.; Fernandez, Margarita; Yamaguchi, Aika; Takano, Yoshihito; Matsuoka, Kazumi; Nezan, Elisabeth; Bilien, Gwenael; Okolodkov, Yuri; Koike, Kazuhiko; Hoppenrath, Mona; Pfaff, Maya; Pitcher, Grant; Al-muftah, Abdulrahman; Rochon, Andre; Lim, Po Teen; Leaw, Chui Pin; Lim, Zhen Fei; Ellegaard, Marianne.
trains of a dinoflagellate from the Salton Sea, previously identified as Protoceratium reticulatum and yessotoxin producing, have been reexamined morphologically and genetically and Pentaplacodinium saltonense n. gen. et sp. is erected to accommodate this species. Pentaplacodinium saltonense differs from Protoceratium reticulatum (Claparède et Lachmann 1859) Bütschli 1885 in the number of precingular plates (five vs. six), cingular displacement (two widths vs. one), and distinct cyst morphology. Incubation experiments (excystment and encystment) show that the resting cyst of Pentaplacodinium saltonense is morphologically most similar to the cyst-defined species Operculodinium israelianum (Rossignol, 1962) Wall (1967) and O. psilatum Wall (1967)....
Tipo: Text Palavras-chave: Pentaplacodinium; Protoceratium; Precingular plates; Salton Sea; Ceratocorys; Operculodinium; Cribroperidinioideae.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00416/52756/53852.pdf
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Prorocentrum bimaculatum sp nov (Dinophyceae, prorocentrales), a new benthic dinoflagellate species from Kuwait (Arabian gulf) ArchiMer
Chomerat, Nicolas; Saburova, Maria; Bilien, Gwenael; Al-yamani, Faiza.
A new benthic dinoflagellate species, Prorocentrum bimaculatum sp. nov., is studied from Kuwaits marine sediments, based on detailed morphological and molecular data. Cells are large, oblong oval in shape. They are 49.955.3 mu m long and 38.443.2 mu m wide. The ornamentation of this new species is peculiar, and characterized by smooth valves with large pores (0.320.50 mu m) scattered on their surface, except in two circular patches of similar to 15 mu m in diameter, devoid of ornamentation and located on both sides of the valve centers. The periflagellar area is widely triangular, located in a moderate excavation of the right valve, and comprises nine platelets. The intercalary band of P. bimaculatum is smooth. The molecular phylogenetic position of this...
Tipo: Text Palavras-chave: Arabian Gulf; Benthic dinoflagellates; Kuwait; Molecular phylogeny; Morphology; Nuclear DNA; Prorocentrum; RDNA; SEM; Taxonomy.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00066/17756/15698.pdf
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Pseudo-nitzschia australis on French Atlantic coast - an unusual toxic bloom ArchiMer
Nezan, Elisabeth; Chomerat, Nicolas; Bilien, Gwenael; Boulben, Sylviane; Duval, Audrey; Ryckaert, Mireille.
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11949/8657.pdf
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