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| Brugirard-Ricaud, K.; Duchaud, E.; Givaudan, A.; Girard, P.A.; Kunst, F.; Boemare, N.; Brehélin, M.; Zumbihl, R.. |
| Photorhabdus is an entomopathogenic bacterium belonging to the Enterobacteriaceae. The genome of the TT01 strain of Photorhabdus luminescens was recently sequenced and a large number of toxin-encoding genes were found. Genomic analysis predicted the presence on the chromosome of genes encoding a type three secretion system (TTSS), the main role of which is the delivery of effector proteins directly into eukaryotic host cells. We report here the functional characterization of the TTSS. The locus identified encodes the secretion/translocation apparatus, gene expression regulators and an effector protein - LopT - homologous to the Yersinia cysteine protease cytotoxin YopT. Heterologous expression in Yersinia demonstrated that LopT was translocated into mammal... |
| Tipo: Journal Article |
Palavras-chave: ENTOMOPATHOGENIC BACTERIUM; GENE EXPRESSION; ENTOMOPATHOGENIC NEMATODE; INSECT CELL MEMBRANE. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e8fb85eb&uri=/notices/prodinra1/2009/03/ |
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| Mracek, Z.; Nguyen, K.B.; Tailliez, P.; Boemare, N.; Chen, S.. |
| Steinernema sichuanense n. sp. is characterized by male, female and IJ. For male, the spicules are robust with prominent rostrum; gubernaculum has blunt anterior end; cuneus is arrow-shaped, pointed posteriorly. Second-generation male has a prominent mucron. For female, tail usually has one to four papillae-like projections on tail tip; post anal swelling is absent. For U, body length is about 7 10 gm; lateral field has six ridges; the formula of lateral field is 2, 5, 6, 4, 2 with two prominent submarginal ridges; tail usually has a dorsal depression. In Steinernema affine/intermedium group, the U of S. sichuanense n. sp. differs from S. affine by its absence of the internal tail spine; differs from Steinernema beddingi by its six ridges in lateral field... |
| Tipo: Journal Article |
Palavras-chave: DESCRIPTION; ENTOMOPATHOGENIC NEMATODE; INSECT PARASITE; MOLECULAR; NEW SPECIES; PHYLOGENETIC SYSTEMATIC; STEINERNEMA; XENORHABDUS; TAXONOMY. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008a03b64c&uri=/notices/prodinra1/2009/03/ |
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| Gaudriault, S.; Duchaud, E.; Lanois, A.; Canoy, A.S.; Bourot, S.; DeRose, R.; Kunst, F.; Boemare, N.; Givaudan, A.. |
| The bacterium Photorhabdus establishes a highly specific association with Heterorhabditis, its nematode host. Photorhabdus strains associated with Heterorhabditis bacteriophora or Heterorhabditis megidis were compared using a Photorhabdus DNA microarray. We describe 31 regions belonging to the Photorhabdus flexible gene pool. Distribution analysis of regions among the Photorhabdus genus identified loci possibly involved in nematode specificity |
| Tipo: Journal Article |
Palavras-chave: PHOTORHABDUS; HETERORHABDITIS; GENE POOL; GENOME SEQUENCE; HYBRIDIZATION; CHROMOSOMAL SEQUENCE. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200879731a17&uri=/notices/prodinra1/2009/01/ |
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