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Cloning and retinal expression of melatonin receptors in the European sea bass, Dicentrarchus labrax ArchiMer
Sauzet, S; Besseau, L; Perez, P; Coves, Denis; Chatain, Beatrice; Peyric, E; Boeuf, G; Munoz Cueto, J; Falcon, J.
Melatonin contributes to synchronizing behaviors and physiological functions to daily and seasonal rhythm in fish. However, no coherent vision emerges because the effects vary with the species, sex, moment of the year or sexual cycle. And, scarce information is available concerning the melatonin age, receptors, which is crucial to our understanding of the role melatonin plays. We report here the full length cloning of three different melatonin receptor subtypes in the sea bass Dicentrarchus labrax, belonging, respectively, to the MT1, MT2 and Mellc subtypes. MT1, the most abundantly expressed, was detected in the central nervous system, retina, and gills. MT2 was detected in the pituitary gland, blood cells and, to a lesser extend, in the optic tectum,...
Tipo: Text Palavras-chave: Retina; Melatonin receptors; Sea bass.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4513.pdf
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Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph ArchiMer
Payan, P; Borelli, G; Priouzeau, F; De Pontual, Helene; Boeuf, G; Mayer Gostan, N.
Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the endolymph calcium of 200% h(-1). Ca2+ entry into the proximal endolymph was faster than into the distal fluid. Net Ca2+ movement across the saccular epithelium depended on the direction and intensity of the chemical gradient of calcium between the Ringer solution and the endolymph. Increasing the calcium concentration in the Ringer solution up to 4.4 mmol l(-1) provoked an accumulation of Ca2+ in both proximal and distal endolymphs, and equilibrium was reached...
Tipo: Text Palavras-chave: Inner ear; Perfusion; Otolith; Endolymph; Flux; Calcium; Oncorhynchus mykiss; Trout.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-416.pdf
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