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Artificial insemination system without estrous observation in suckled beef cows Ciência Rural
Borges,Luiz Felipe Kruel; Ferreira,Rogério; Siqueira,Lucas Carvalho; Bohrer,Rodrigo Camponogara; Borstmann,Jacson William; Oliveira,João Francisco Coelho de; Gonçalves,Paulo Bayard Dias.
The aim was to develop a timed artificial insemination (TAI) system in suckled beef cows. Cows (n=227), 60-80 days postpartum, received estradiol benzoate (5mg) and a vaginal device containing 250µg of medroxyprogesterone acetate (MPA; day 0). On day six, cloprostenol (125µg) and eCG (400IU) were administrated and calves were weaned for 88h. The devices were removed on day seven (BioRep group) or on day eight (TAI group). All cows of TAI group and cows of BioRep group that did not exhibit standing estrus received GnRH (100µg) on day 9. In experiment I, the follicular growth was monitored daily by transrectal ultrasound exams, from day 6 to day 9. The average size of the dominant follicle on day nine was 11.1±0.99mm (BioRep, n=7) and 11.5±0.65mm (TAI, n=7)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cows; Postpartum; TAI; MPA; Ovulation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000200028
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Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes Ciência Rural
Rosa,Paulo Roberto Antunes da; Bohrer,Rodrigo Camponogara; Ludke,Charles Alencar; Cesaro,Matheus Pedroti De; Pereira,Gabriel Ribas; Mondadori,Rafael Gianela; Antoniazzi,Alfredo Quites; Gonçalves,Paulo Bayard Dias.
The objective of this study was to investigate the mRNA expression and protein localization of Grb10 gene in bovine cumulus-oocyte complexes (COCs) from different follicle sizes. Firstly, it was investigated the mRNA expression to correlate with maturation rates. COCs from follicles at 1-3, 4-6, 6-8 and >8mm were used to evaluate Grb10 gene expression by qRT-PCR assay and nuclear maturation rates. It was observed that more competent oocytes (from follicles at 6-8 and >8mm; P>0.05), had lower Grb10 mRNA expression levels when compared to the oocytes from follicles at 1-3 and 4-6mm (P>0.05). After it was performed an immunofluorescence analysis in COCs from different follicle sizes (1-3, 4-6, 6-8 and >8mm) to investigate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: : mRNA; Protein; Maturation; Oocyte; Grb10..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500898
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