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| Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier. |
| Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a... |
| Tipo: Text |
Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf |
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| Oleaga, Ana; Rey, Olivier; Polack, Bruno; Grech-angelini, Sébastien; Quilichini, Yann; Pérez-sánchez, Ricardo; Boireau, Pascal; Mulero, Stephen; Brunet, Aimé; Rogon, Anne; Vallée, Isabelle; Kincaid-smith, Julien; Allienne, Jean-françois; Boissier, Jérôme. |
| Environmental and anthropogenic changes are expected to promote emergence and spread of pathogens worldwide. Since 2013, human urogenital schistosomiasis is established in Corsica island (France). Schistosomiasis is a parasitic disease affecting both humans and animals. The parasite involved in the Corsican outbreak is a hybrid form between Schistosoma haematobium, a human parasite, and Schistosoma bovis, a livestock parasite. S. bovis has been detected in Corsican livestock few decades ago raising the questions whether hybridization occurred in Corsica and if animals could behave as a reservoir for the recently established parasite lineage. The latter hypothesis has huge epidemiological outcomes since the emergence of a zoonotic lineage of schistosomes... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00504/61566/65491.pdf |
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| Angora, Etienne K.; Boissier, Jérôme; Menan, Hervé; Rey, Olivier; Tuo, Karim; Touré, Andre O.; Coulibaly, Jean T.; Méité, Aboulaye; Raso, Giovanna; N’goran, Eliézer K.; Utzinger, Jürg; Balmer, Oliver. |
| Schistosomiasis is a parasitic disease affecting more than 250 million people, primarily in sub-Saharan Africa. In Côte d’Ivoire both Schistosoma haematobium (causing urogenital schistosomiasis) and Schistosoma mansoni (causing intestinal schistosomiasis) co-exist. This study aimed to determine the prevalence of S. haematobium and S. mansoni and to identify risk factors among schoolchildren in the western and southern parts of Côte d’Ivoire. From January to April 2018, a cross-sectional study was carried out including 1187 schoolchildren aged 5–14 years. Urine samples were examined by a filtration method to identify and count S. haematobium eggs, while stool samples were subjected to duplicate Kato-Katz thick smears to quantify eggs of S. mansoni and... |
| Tipo: Text |
Palavras-chave: Côte d’Ivoire; Prevalence; Risk factors; Schistosoma haematobium; Schistosoma mansoni; Schistosomiasis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61953/66043.pdf |
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| Guegan, Hélène; Fillaux, Judith; Charpentier, Elena; Robert-gangneux, Florence; Chauvin, Pamela; Guemas, Emilie; Boissier, Jérôme; Valentin, Alexis; Cassaing, Sophie; Gangneux, Jean-pierre; Berry, Antoine; Iriart, Xavier. |
| Background The diagnosis of schistosomiasis currently relies on microscopic detection of schistosome eggs in stool or urine samples and serological assays. The poor sensitivity of standard microscopic procedures performed in routine laboratories, makes molecular detection methods of increasing interest. The aim of the study was to evaluate two in-house real-time Schistosoma PCRs, targeting respectively S. mansoni [Sm] and S. haematobium [Sh] in excreta, biopsies and sera as potential tools to diagnose active infections and to monitor treatment efficacy. Methods Schistosoma PCRs were performed on 412 samples (124 urine, 86 stools, 8 biopsies, 194 sera) from patients with suspected schistosomiasis, before anti-parasitic treatment. Results were compared to... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62534/66865.pdf |
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