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Registros recuperados: 18
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Biophysical and biochemical properties of an unusual birnavirus pathogenic for rotifers ArchiMer
Comps, Michel; Mari, Jocelyne; Poisson, Francois; Bonami, Jean-robert.
A cytoplasmic dsRNA virus, rotifer birnavirus (RBV), has recently been isolated from the rotifer Brachionus plicatilis and is associated with a high mortality rate. Histologically, the viral lesions consist of characteristic inclusions, particularly amorphous dense bodies containing occluded particles. Purified virions are about 59 nm in diameter, single-shelled and display four capsomers per edge. The purified virions have a buoyant density of 1.290 (full particles) and 1.250 (empty particles) in CsCI gradients. Four major structural polypeptides of Mrs 60K, 52K, 33K and 27K were detected by SDS-PAGE. The genome is composed of two linear segments of dsRNA with Mrs of 2.45 x 106 and 2.31 × 106; additionally, small circular ssRNA molecules were detected by...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/00129/24032/21994.pdf
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Développement de protistes parasites en culture cellulaire de l'huître japonaise Crassostrea gigas ArchiMer
Cousserans, François; Bonami, Jean-robert; Comps, Michel; Grizel, Henri.
Cell cultures, which are the basis of numerous recent studies in medical pathology, have been, for a few years, of great interest in the studies carried out on the diseases affecting invertebrates. However, such cultures, dealing with marine bivalve molluscs, obtained for the first time by Vago and Chastang (1) and progressively improved since then [(2) to (10)], have not been used in pathological studies yet. As the diseases affecting marine molluscs of economic importance such as oysters are both of great interest and facing specific study difficulties, we tried to adapt oyster cell cultures to the study of the presence, development and cycles of the pathogenic or possibly pathogenic microorganisms affecting Crassostrea, and especially the species gigas,...
Tipo: Text Palavras-chave: Culture cellule; Parasite; Huîtres; Crassostrea gigas.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-5907.PDF
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Etude d'un parasite de la glande digestive observé au cours de l'épizootie actuelle de l'huître plate ArchiMer
Grizel, Henri; Comps, Michel; Cousserans, François; Bonami, Jean-robert; Vago, Constantin.
Description, in electron microscopy, of the stages of a parasite observed in the digestive gland of the oyster Ostrea edulis during the epizooties which are currently spreading throughout flat oyster farms.
Tipo: Text Palavras-chave: Pathologie; Ostrea edulis; Huitre; Epizootie; Parasite.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-5921.PDF
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Infection virale associée à des mortalités chez l'huître Crassostrea gigas Thunberg ArchiMer
Comps, Michel; Bonami, Jean-robert.
Some viral particles close to the lridovirus are described in the cytoplasm of some connective cells in the Japanese oyster Crassostrea gigas. This virus looks similar to the one described in Crassostrea angulata and associated with the mortalities of 1970-1973 and to the one observed in the gill damages in the same species.
Tipo: Text Palavras-chave: Virus; Mortalité; Pathologie; Crassostrea gigas; Huître.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/1977/publication-6082.PDF
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La mise en évidence d'une infection virale chez l'huître portugaise à l'occasion de l'épizootie de 1970-1974 ArchiMer
Comps, Michel; Bonami, Jean-robert; Vago, C.; Razet, Daniel.
Among the major epizootics known in marine Molluscs, the one that started on the coasts of Europe in 1970 in the Portuguese oyster, Crassostrea angulata, was certainly one of the largest due to its range and due to the economic loss sustained by the French oyster farming as a whole. It led to the disappearance of the species C. angulata as a farmed oyster, and to the massive introduction of the Japanese oyster, C. gigas, known to be resistant to the disease. From the first manifestations of this epizootic, the laboratories of the ISTPM were actively dedicated to its etiology. Now, after several years, a study begun at the La Tremblade laboratory has just been completed.
Tipo: Text Palavras-chave: Marennes Oléron; Mortalités; Histologie; Epizootie; Crassostrea angulata.
Ano: 1976 URL: http://archimer.ifremer.fr/doc/1976/publication-2995.pdf
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Mass mortalities in hatchery-reared sea bass (Lates calcarifer) larvae associated with the presence in the brain and retina of virus-like particles ArchiMer
Renault, Tristan; Haffner, Philippe; Baudin Laurencien, F.; Breuil, Gilles; Bonami, Jean-robert.
Since 1986, an unknown disease causing high mortalities appeared in Ifremer's Pacific OceanologicaI Center (Tahiti) nursery of sea bass (Lates calcarifer). opathology of lesions involving both centraI nervous system (CNS) and eye retina, and the tuation exists in South-East Asian countries (Indonesia, Philippines, Malaysia and Singapore), and in northem Australia. This paper describes the histmorphology of a new fish virus, which is believed to be the aetiological agent of high mortalities among sea bass larvae.
Tipo: Text Palavras-chave: Virus like; Lates calacrifer; Hatchery; Mortality.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2885.pdf
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Mise en evidence de particules d'allure virale associees aux noyaux des cellules mesodermiques de la zone germinative testiculaire du crabe Rhithropanopeus harrisii (Gould) (Brachyoure, Xanthide). ArchiMer
Payen, Geneviève G.; Bonami, Jean-robert.
Ultrastructural study of the testicular germinative zone in the crab R. harrisii has led to observation of virus-like particles exclusively located in the nuclei of mesodermal cells encompassing primary spermatogonia. These particles are related to the group of viruses of the herpes type.
Tipo: Text Palavras-chave: Rhithropanopeus harrisii; Viruses; Testes; Viral diseases.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/publication-1940.pdf
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Mise en évidence d'une infection rickettsienne chez les huîtres ArchiMer
Comps, Michel; Bonami, Jean-robert; Vago, Constantin.
Un microorganisme procaryote a été observé dans le cytoplasme des cellules de diverticules digestifs de l'huître Crassostrea gigas . L'étude des caractéristiques du germe permet de considérer celui-ci comme appartenant aux Rickettsiales sans cycle de type chlamydien.
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Pathologie; Rickettsie; Histologie.
Ano: 1977 URL: http://archimer.ifremer.fr/doc/00041/15241/12559.pdf
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Particules virales de type Bacilovirus observees chez le crabe Carcinus maenas. ArchiMer
Bazin, F.; Monsarrat, P.; Bonami, Jean-robert; Croizier, G.; Meynadier, G.; Quiot, J.m.; Vago, C..
A new virus from the Crab Carcinus maenas has been found in the nuclei of conjunctive and blood cells. Its localization, morphology, size, morphogeny relate it to the Baculovirus group. By some aspects it appears to be related closer to the insect viruses from Oryctes rhinoceros, and to red mite viruses from Panonychus ulmi and P.citri than to the nuclear polyhedrosis of the pink shrimp Penaeus duorarum.
Tipo: Text Palavras-chave: Penaeus duorarum; Panonychus citri; Panonychus ulmi; Oryctes rhinoceros; Carcinus maenas; Viruses; Viral diseases.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-2041.pdf
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Purification and characterization of infectious myonecrosis virus of penaeid shrimp ArchiMer
Poulos, Bonnie; Tang, K; Pantoja, C; Bonami, Jean-robert; Lightner, D.
The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953)...
Tipo: Text Palavras-chave: Purification; Myonecrosis; Virus; Shrimp; Penaeid.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1504.pdf
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Quantitative relationship of two viruses (MrNV and XSV) in white-tail disease of Macrobrachium rosenbergii ArchiMer
Zhang, Hj; Wang, Hm; Yuan, Jf; Li, Lj; Zhang, Jh; Bonami, Jean-robert; Shi, Zl.
Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) were purified from diseased freshwater prawns M rosenbergii and used to infect healthy post-larvae (PL) by an immersion method. Three groups of prawns were challenged with various combined doses of MrNV and XSV. Signs of white-tail disease (WTD) were observed in Groups 1 and 2, which had been challenged with combinations containing relatively high proportions of MrNV and low proportions of XSV. By contrast there was little sign of WTD in Group 3, which had been challenged with a higher proportion of XSV than MrNV. A 2-step Taqman real-time RT-PCR was developed and applied to quantify viral copy numbers in each challenged PL. Results showed that genomic copies of both viruses were much...
Tipo: Text Palavras-chave: White tail disease; Real time RT PCR; Extra small virus; Nodavirus; Macrobrachium rosenbergii.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3568.pdf
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Recherche sur la paralysie virale du crustace decapode Macropipus depurator L. ArchiMer
Bonami, Jean-robert.
The trembling of legs followed by increasing paralysis and eventual death, were recorded on specimens of the above sp from Sete region. EM studies of tissue showed paraspherical bodies measuring approx 61 nm with a size range of 58-65 nm. By experimentally infecting specimens with a purified viral suspension the following pathogenesis was observed: in greater than 60 per cent of the specimens, trembling of the legs began within 6 days, growing very pronounced on the 8th day, leading to a general paralysis and 9 days after the infection a mortality rate of 70-80 per cent was recorded. The following characteristics of the virus are noted: its cytoplasmic localisation, paraspherical form, size ranging from 60-70 nm and the absence of an envelope. Considering...
Tipo: Text Palavras-chave: Paralysis; Virus; Decapoda.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/publication-2151.pdf
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Recherche sur l'agent de la maladie de la glande digestive de Ostrea edulis Linné ArchiMer
Grizel, Henri; Comps, Michel; Bonami, Jean-robert; Cousserans, F.; Duthoit, Jean-louis; Le Pennec, Marie-annick.
Numerous diseases have been described in Ostrea and Crassostrea oysters and especially in cultivated species of commercial interest. The lack of growth, but more often mortalities, are in most cases the most obvious symptoms of this supposed sickness. The consequences of these epizooties can be so bad that they can result in the disappearance of an entire oyster population from a determined area as for Ostrea edulis L. in 1920-1921 (Dollfus, 1922) on some coasts of Europe and for Crassostrea angulata LMK in 1970-1971 on the French Atlantic coast and especially in the Marennes-Oléron and Arcachon basins. These cases can be considered as extreme cases as most of the time, oysters are only subjected to a number of episodic affections, affecting the farms only...
Tipo: Text Palavras-chave: Glande digestive; Maladie; Pathologie; Ostrea edulis; Huître.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-6286.PDF
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Recherches sur une maladie epizootique de l'huitre plate Ostrea edulis Linne ArchiMer
Bonami, Jean-robert; Grizel, Henri; Vago, Constantin; Dutholt, Jean-louis.
Digestive glands of Ostrea edulis affected by an epizootic have been examined by light- and electron- microscopy. Round, compact lesions in the digestive gland cells contain refractive elements staining slightly blue or red with Geimsa. Ultra-structurally, each lesion contains (a) dense cytoplasma, without mitochondria and with paracrystal-line inclusions, (b) enclosed regions with vacuolar cytoplasm, no mitochondria and two types of formation; one is opaque, the other a vacuole containing ribosomes, lamellar structures and peripheral ovoid particles. The nature and significance of these structures is not known.
Tipo: Text Palavras-chave: Histology; Epizooty; Ostrea edulis.
Ano: 1971 URL: http://archimer.ifremer.fr/doc/1971/publication-2238.pdf
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Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses ArchiMer
Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert.
The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of...
Tipo: Text Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf
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Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes ArchiMer
Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V.
The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No...
Tipo: Text Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf
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Une virose de l'huître portugaise (Crassostea angulata LMK) ArchiMer
Comps, Michel; Bonami, Jean-robert; Vago, Constantin; Campillo, Albert.
A viral infection has been observed in the Portuguese oyster Crassostrea angulata during the massive mortalities of 1970-1973. A new type of virus and the viral morphogenesis are described. It is the first virus observed in oysters and linked to an epizooty in this mollusc of great economic importance.
Tipo: Text Palavras-chave: Infection virale; Crassostrea angulata; Huître; Virologie.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-6084.PDF
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Une virose de l'huître portugaise (Crassostrea angulata LMK) ArchiMer
Comps, Michel; Bonami, Jean-robert; Vago, Constantin.
Une infection virale a été observée chez les Huîtres portugaises Crassostrea angulata durant les mortalités massives de 1970-1973. Un type nouveau de virus et la morphogenèse virale sont décrits. Il s'agit du premier virus signalé chez les huîtres, mis en relation avec une épizootie de ce mollusque de ce mollusque économiquement très important.
Tipo: Text Palavras-chave: Pathologie; Virologie; Mollusques bivalves; Crassostrea angulata.
Ano: 1976 URL: http://archimer.ifremer.fr/doc/00087/19820/17462.pdf
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