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Improved detection of Beet necrotic yellow vein virus in a DAS ELISA by means of antibody single chain fragments (scFv) which were selected to protease-stable epitopes from phage display libraries Inra
Uhde, K.; Kerschbaumer, R.J.; Koenig, R.; Hirsch, S.; Lemaire, O.; Boonham, N.; Roake, W.; Himmler, G..
 The detection of Beet necrotic yellow vein virus (BNYVV) instored sugar beets by means of monoclonal antibodies or antibody singlechain fragments (scFv) often poses problems, because the immunodominantC-terminal epitope of the viral coat protein is readily lost due toproteolysis. Clones which produce scFv specific for protease-stableBNYVV epitopes were selected from two naive phage display libraries.Fusion proteins of the scFv with a human IgG kappa chain (expressed fromthe newly designed vector pCL) or with alkaline phosphatase,respectively, allow the ELISA detection of BNYVV even in stored sugarbeets with a sensitivity which was comparable or often higher than thatachieved with polyclonal antibodies
Tipo: Journal Article Palavras-chave: BNYVV; BEET NECROTIC YELLOW VEIN VIRUS; EPITOPE; COQUE VIRALE; VIRUS DES VEGETAUX; ANTICORPS MONOCLONAL; TEST ELISA.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100016386085053&uri=/notices/prodinra1/2010/11/
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QBOL: a new EU project focusing on DNA barcoding of Quarantine organisms Inra
Bonants, P.; Groenewald, E.; Rasplus, J.Y.; Maes, M.; de Vos, P.; Frey, J.; Boonham, N.; Nicolaisen, M.; Bertacini, A.; Robert, V.; Barker, I.; Kox, L.; Ravnikar, M.; Tomankova, K.; Caffier, D.; Li, M.; Armstrong, K.; Freitas-Astua, J.; Stefani, E.; Cubero, J.; Mostert, L..
In 2009 a new three year EU funded project (QBOL) started on DNA barcoding of important plant pests. An international consortium of 20 partners (universities, research institutes, and phytosanitary organizations) from around the world, coordinated by Plant Research International (Wageningen, the Netherlands), will collect DNA barcodes from many plant pathogenic quarantine organisms, store these sequences in a database accessible over the internet, develop a DNA bank and train end-users. All these activities should help National Plant Protection Services in the correct identification and detection of plant pathogenic quarantine organisms
Tipo: Journal Article Palavras-chave: ORGANISME DE QUARANTAINE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010de96c892&uri=/notices/prodinra1/2010/12/
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