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Activité respiratoire en continu de l'huître Crassostrea gigas : mise au point d'une nouvelle méthodologie ArchiMer
Bougrier, Serge; Geairon, Philippe.
A continuous monitoring system to measure the respiration of Crassostrea gigas has been developped. The metabolic function estimated by oxygen consumption has been monitored during periods of 24 h, without food, at a temperature constant of 15°C. Preliminary results showed that the mean percentage of oyster activities was 50 % with a high variability from 0 to 100 %. The number of activity periods per day (24 h) is function of the oysters with variation between 0 and 7.
Tipo: Text Palavras-chave: Physiology; Respiration; Crassostrea gigas; Oysters; Physiologie; Respiration; Crassostrea gigas; Huîtres.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3046.pdf
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Alimentation expérimentale de l'huître Crassostrea gigas à l'aide de navicules bleus Haslea ostrearia (Simonsen) de différentes tailles ArchiMer
Barille, Laurent; Bougrier, Serge; Geairon, Philippe; Robert, Jean-michel.
The influence on the feeding physiology (clearance, filtration, ingestion, absorption) of the Japanese oyster Crassostrea gigas of low concentrations of suspended particulate matter (SPM ranging from 2 to 20 mg.l-l) and high food quality (mean organic fraction =54 %) was studied in the laboratory. Experimental diets were made up with three strains of the "blue diatom" Haslea ostrearia, which is responsible of the greening of oyster-ponds. The strains were characterized by different sizes (48, 65 and 98 µm respectively) but possessed the same density and biochemical composition. Clearance rate was found constant at 2.2 1.h(-l).g-l dry weight and therefore was not influenced by increasing seston load. Pseudofaeces production was observed for SPM...
Tipo: Text Palavras-chave: Taille des particules; Qualité alimentaire; Haslea ostrearia; Crassostrea gigas; Alimentation; Particle size; Food quality; Haslea ostrearia; Crassostrea gigas; Feeding.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3070.pdf
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Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Rapport année 1995 ArchiMer
Boudry, Pierre; Naciri, Yamama; Heral, Maurice; Collet, Bertrand; Bougrier, Serge; Geffard, Olivier; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre.
Le programme de recherche présenté ci-dessous est le fruit d'une réflexion conjointe entre généticiens et physiologistes afin de mettre sur pied un ensemble d'expériences permettant d'étudier les bases génétiques de la variabilité des caractères impliqués dans la croissance chez l'huître creuse, Crassostrea gigas.
Tipo: Text Palavras-chave: Génétique; Sélection génétique; Génétique des populations; Croissance; Crassostrea gigas.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15518/12905.pdf
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Bilan énergétique de l'huître creuse (Crassostrea gigas) durant la période de forte croissance en mai en Baie de Marennes-Oléron (France) ArchiMer
Soletchnik, Patrick; Heral, Maurice; Razet, Daniel; Bougrier, Serge; Geairon, Philippe.
Ecophysiological study was conducted on the japanese oyster (Crassostrea gigas) reared in Marennes-Oléron bay (France), during May 1992. Most of the somatic growth of the oyster occured during that month. The measurements were achieved from a removable laboratory stated in Le Chapus for 3 days at different tide level (coefficient of 49, 57, and 88). The food conditions were monitored through a computer with a continuous record of the turbidity during aIl the tidal cycle. In spite of an important variation between the food conditions in mean seston load, from 13,5 to 54,6 mg/!, the available organic matter ranged from 3 to 4,9 mg/l. The rate of organic matter in the water ranged 22 % to 9 %. The organic selection efficiency was significantly higher for the...
Tipo: Text Palavras-chave: Energetic budget; Selection efficiency; Filtration; Crassostrea gigas; Budget énergétique; Sélection; Consommation; Crassostrea gigas.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/acte-3068.pdf
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Bilan énergétique de l'huître japonaise Crassostrea gigas en conditions expérimentales de laboratoire au cours du mois de septembre 1991 ArchiMer
Soletchnik, Patrick; Bougrier, Serge; Razet, Daniel; Geairon, Philippe; Prou, Jean; Heral, Maurice; Barille, Laurent.
L'étude ci dessous, réalisée chez Crassostrea gigas, a comme objectif de comparer l'effet de différents types d'aliments sur le bilan énergétique de cette espèce.
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Bilan énergétique; Sources alimentaires; Biodépots; Tri pré-ingestif.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00100/21095/18720.pdf
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Characteristics of the particulate matter during the Mai '92 experiment. Effect on ecophysiological functions on mussel and oyster populations. ArchiMer
Prou, Jean; Soletchnik, Patrick; Heral, Maurice; Geairon, Philippe; Razet, Daniel; Bougrier, Serge.
In order to complete the knowledge of seasonal variability of ecophysiological parameters, in situ experiment was conducted in May 1992 at the sampling site "Le Chapus". As in the previous experiments (May '91, October '91 and February '92), the race-way technique was used to study ecophysiological behavior of oyster and mussel populations submitted to natural variability.
Tipo: Text Palavras-chave: Pacific cupped oyster; Crassotrea gigas; Particulate matter; Pigments; Particle size distribution; Retention; Filtration; Mytilus edulis.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00100/21091/18715.pdf
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Comparison of growth of mussel, Mytilus edulis, on longline, pole and bottom culture sites in the Pertuis Breton, France ArchiMer
Garen, Pierre; Robert, Stephane; Bougrier, Serge.
Growth of the mussel, Mytilus edulis, was compared for the first time under three culture regimes, longline located in open sea, pole and on-bottom both situated on an intertidal flat, in the Pertuis Breton, on the Atlantic coastline of France. Mussel sampling was performed on a monthly basis over 1 year while monitoring of hydrobiological parameters was conducted on a biweekly basis. Fluctuation of environmental parameters showed a similar pattern on both sea and shore locations with generally higher concentration levels for the intertidal area compared with the open sea, i.e. 4.07 and 3.17 mg 1(-1) for particulate organic matter, 3.16 and 2 mug 1(-1) for chlorophyll-a, 58.43 and 38.72 mg 1(-1) for inorganic-N, respectively. A clear seasonal growth...
Tipo: Text Palavras-chave: Condition index; Emersion; Food resource; Water quality; Growth; Mytilus edulis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-581.pdf
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Compte rendu de la réunion des 14 et 15 Avril 1993 - La Tremblade - Réseau Génétique Mollusques (REGEMO) ArchiMer
Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie.
La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en...
Tipo: Text Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf
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Compte-rendu et recommandations de l'atelier RINBIO tenu au Centre de Nantes les 19 et 20 janvier 1997 - De l'usage de la méthode des transplants de moule pour le suivi de la contamination chimique le long des côtes méditerranéennes françaises ArchiMer
Cossa, Daniel; Abarnou, Alain; Andral, Bruno; Bougrier, Serge; Buestel, Dominique; Claisse, Didier; Hatt, Philippe-jacques; Stanisiere, Jean-yves.
Ce rapport fait suite à la création par les directions DEL et DRV de l'IFREMER d'un groupe de travail, dit RlNBIO, chargé d'étudier les implications de l'usage des transplants de populations de moules pour évaluer la contamination chimiques des zones côtières méditerranéennes. L'objectif du Réseau Intégrateurs Biologiques (RlNBIO) est en effet d'obtenir une évaluation des niveaux de la contamination chimique à l'échelle du bassin méditerranéen français. La méthodologie s'appuie sur la mesure des concentrations en contaminants métalliques et organiques bioaccumulés dans les tissus de moules transplantées (Mytilus galloprovincialis) pendant plusieurs dans les zones à étudier. Or, la phase exploratoire a mis en évidence une interdépendance entre les niveaux...
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Ano: 1998 URL: http://archimer.ifremer.fr/doc/00086/19715/17356.pdf
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Contribution à l'étude de la variabilité phénotypique et génétique des crustacés isopodes (Idotea balthica Pallas et Metoponorthus pruinosus Brandt) et pénéides (Penaeus japonicus Bate) ArchiMer
Bougrier, Serge.
Les travaux, mentionnés dans ce mémoire, ont été effectués au laboratoire de Biologie Animale, "Physiologie et Génétique des Crustacés", de l'Université de Poitiers. Nous avons entrepris dans une première partie la comparaison morphologique de trois populations (une togolaise et deux françaises), dont nous mettons en doute l'appartenance de la même espèce Metoponorthus pruinosus. En effet des essais de croisements (JUCHAULT et KOUIGAN), communications personnelles) entre diverses populations africaines d'une part et françaises d'autre part ne fournissent aucune descendance, bien que dans certains il y ait accouplement. Le but de notre étude est donc de mettre en évidence les différences statistiquement significatives, qui peuvent exister entre les...
Tipo: Text
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00451/56300/57888.pdf
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Does the prevalence of Bonamia and Marteilia diseases be reduced on flat oysters (Ostrea edulis) of Atlantic and Mediterranean origin, when they are reared together with the Japanese oyster (Crassostrea gigas) in tidal ponds? ArchiMer
Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge.
The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and...
Tipo: Text Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf
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Ecophysiologie in situ de l'huître Crassostrea gigas dans un milieu macrotidal (bassin de Marennes-Oléron - Chapus 1991) ArchiMer
Guezennec, Loic; Prou, Jean; Heral, Maurice; Razet, Daniel; Bougrier, Serge; Soletchnik, Patrick.
L'expérience menée lors du stage fait suite à deux expériences similaires menées en 1988 et 1990. Elle vise à étudier le comportement d'une population d'huîtres (100 individus) soumise aux variations du milieu naturel. La durée de l'expérience (3 semaines) permet de tester les effets simultanés des cycles haute mer. basse mer et celui des vives·eaux. mortes-eaux. De plus. les données acquises en continu sur le milieu (fluorescence et turbidité. salinité. température) permettront le calage des modèles courantologiques et sédimentologiques. Le choix du mois de mai pour l'expérience est déterminé par l'apparition. en général. d'un bloom phytoplanctonique à cette période de l'année. Les expériences précédentes (1988 et 1990) ont permis déjà d'appréhender la...
Tipo: Text Palavras-chave: Ecophysiologie; Huître creuse; Crassostrea gigas; Bassin de Marennes-Oléron; Chapus; Charente Maritime.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00102/21308/18921.pdf
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Effect of the density of the culture and the depth on the seasonal variation of the biochemical composition of Crassostrea virginica in patuxent river (Chesapeake Bay) ArchiMer
Prou, Jean; Heral, Maurice; Goulletquer, Philippe; Forget, Maïté; Bougrier, Serge; Razet, Daniel.
The project of cultivation of oyster has been designed to evaluate the growth rate, the mortality, the reproductive effort, production and biochemical composition of the oyster function of the density and the depth. This plan was intended: - to build model of growth rate, - to demonstrate the action of the different parameters on growth rate (temperature, food effect...), - to evaluate the density effect to see if the available food could be a limiting factor, - to estimate the effect of the depth joined to the impact of the high turbidity near the bot tom, - to detect the mortality related to the prevalence of diseases. The project began in July 1989 by dredging 20 000 small oysters at the mouth of the Patuxent River near Cove Point. Three experimental...
Tipo: Text Palavras-chave: Physiology; Seasonal variation; Biochemical composition; Density; Crassostrea virginica; Cheseapeake Bay.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00200/31104/29503.pdf
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Essai de réimplantation de Crassostrea angulata en France. Résistance au chambrage et comportement des hybrides C. angulata - C. gigas ArchiMer
Bougrier, Serge; Raguenes, Gerard; Bachere, Evelyne; Tige, Gilbert; Grizel, Henri.
The inability to rear the oysters C. gigas and O. edulis in certain Brittany waters has prompted us to conduct breeding trials with C. angulata C. gigas and their hybrids. Results have revealed that all the strains tested were sensitive to Tributyl tin (TBT), showing atnormal shell thickness and their growth was lower than C. gigas. In addition, C. angulata was still affected by the presence of the Iridovirus in France.
Tipo: Text Palavras-chave: TBT; Breeding trials; Hubrids; Crassostrea gigas; Crassostrea angulata; TBT; Chambrage; Croisement; Hybrides; Crassostrea gigas; Crassostrea angulata.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-3102.pdf
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Etude des relations allométriques de l'huître plate (Ostrea edulis) relatives à la filtration et à la sonsommation d'oxygène en fonction de la température ArchiMer
Haure, Joel; Baud, Jean-pierre; Bougrier, Serge.
Introducing new species for shellfish farming is essential for expanding farmers' markets and for minimising the pathological risks that endangers professional marine aquaculture activities, which traditionally rely on only one species. Ifremer's Aquaculture and Pathological Genetics (GAP) laboratory participates in the mollusc genetics network (REGEMO). Since 1989, this network has been carrying out a selection programme for resistance or tolerance to Bonamia ostreae in European flat oysters (Ostrea edulis) to revitalize cultivation of this species on a national level.
Tipo: Text Palavras-chave: Température; Oxygène; Filtration; Ostrea edulis; Allométrie.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3254.pdf
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Etudes des bases génétiques et de la varibailité des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas ArchiMer
Collet, Bertrand; Boudry, Pierre; Bougrier, Serge; Heurtebise, Serge; Phelipot, Pascal; Ledu, Christophe; Morand, Berenice; Heral, Maurice; Gerard, Andre.
A European programme (GENEPHYS) was launched in 1996 to study the genetic basis and variability of physiological characters involved in growth in Crassostrea gigas. This research programme was a prerequisite for any genetic selection programme on physiological functions. Research groups from Ireland, England, Greece and France are involved in this project and participate in genetic analyses (allozymes and microsatellites) and physiological analyses (ecophysiology, enzymology, stress proteins). Ifremer at La Tremblade was responsible for producing the biological material while Ifremer-CREMA (human-influenced marine ecosystems research centre) was responsible for carrying out non-destructive physiological analyses.
Tipo: Text Palavras-chave: Sélection génétique; Croissance; Crassostrea gias; Génétique.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3243.pdf
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Feeding responses of Crassostrea gigas (Thunberg) to inclusion of different proportions of toxic dinoflagellates in their diet ArchiMer
Bardouil, Michele; Bohec, Madeleine; Bougrier, Serge; Lassus, Patrick; Truquet, Philippe.
An experimental flow-through system allowing determination of the feeding behaviour of individual molluscs was used to study oysters exposed to mixed diets composed of varying proportions of the diatom Thalassiosira weissflogii and two strains (toxic and nontoxic) of the dinoflagellate Alexandrium larnarense. Our results show that, when compared to a T. weissflogii unialgal diet, even a diatomltoxic dinoflagellate ratio as low in biomass as 90/10 reduced clearance rates and biodeposit production by oysters. Consumption was slightly but significantly decreased for a 50/50 diatomltoxic dinoflagellate mixture. For the toxic dinoflagellate unialgal diet, ingestion, absorption and consumption were completely inhibited. Thus, the inclusion of low amounts of...
Tipo: Text Palavras-chave: Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiologie; Toxine paralysante des coquillages; Crassostrea gigas; Thalassiosira weissflogii; Alexandrium tamarense; Ecophysiology; Paralytic shellfish poison.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00017/12788/9729.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - First Progress Report 1st January-31st December 1996 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkinns, N. P.; Zouros, Eleftherios; Bonhomme, Francois.
The EEC project "Genephys 1996-2000", initiated by the IFREMER laboratory GAP (Génetique, Aquaculture et Pathologie, La Tremblade and CREMA (Centre de Recherche en Ecologie Marine et Aquaculture, l'Houmeau), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are: 1- To express the maximum variability of growth, both at the larval stage and at the adult stage, in an oyster population originating from parents of various origins. 2- To check the persistence over the time, of growth performance and related physiological functions. 3-...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00050/16149/13632.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Second Progress Report 1st January-31st December 1997 - Contract n° FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Hawkins, A. J. S.; Thiriot Quievreux, Catherine; Wilkins, N. P.; Zouros, Eleftherios; Bonhomme, Francois; Buestel, Dominique; Samain, Jean-francois.
The UE project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-L'Houmeau (Centre de Recherche en Ecologie marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main objectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Absorption efficiency; Growth; Oysters; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00050/16150/13633.pdf
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Genetic bases and variability of physiological traits involved in growth in Crassostrea gigas - Third Progress Report 1st January-31st December 1998 - Contract N°FAIR 95-421 Commission of the European Communities ArchiMer
Gerard, Andre; Boudry, Pierre; Bougrier, Serge; Buestel, Dominique; Samain, Jean-francois; Hawkins, A. J. S.; Wilins, N.; Zouros, Eleftherios; Bonhomme, Francois; Thiriot Quievreux, Catherine.
The EU project "GENEPHYS", initiated by the IFREMER laboratory GAP-La Tremblade (Génétique Aquaculture et Pathologie) and CREMA-l'Houmeau (Centre de Recherche en Ecologie Marine et Aquaculture), aims to establish the relationships between physiological traits involved in growth (oxygen consumption, absorption efficiency, scope for growth) and their genetic bases (determinism, variability within and among populations) in cupped oyster Crassostrea gigas. The main bjectives are : 1. To express the maximum variability of growth, both at the larval stage and at the adult stage, in a oyster population originating from parents of various origins. 2. To check the persistence over the time, of growth performance and related physiological functions. 3. To explain...
Tipo: Text Palavras-chave: Genetic; Determinism; Variability; Physilogy; Oxygen consumption; Growth; Absorption efficiency; Oysters; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00050/16151/13634.pdf
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