Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 13
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax ArchiMer
Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois.
Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using...
Tipo: Text Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf
Imagem não selecionada

Imprime registro no formato completo
"Bivalvomix" : génomique évolutive des bivalves marins ArchiMer
Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang & Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
Tipo: Text
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf
Imagem não selecionada

Imprime registro no formato completo
Characterisation and expression of four mRNA sequences encoding glutathione S-transferases pi, mu, omega and sigma classes in the Pacific oyster Crassostrea gigas exposed to hydrocarbons and pesticides ArchiMer
Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Hydrocarbon and pesticide pollution in coastal ecosystems can disturb marine bivalve metabolism. In this study, we characterised four full-length cDNA sequences encoding glutathione S-transferases (GSTs) in the Pacific oyster Crassostrea gigas. A BLAST X search showed that these four sequences encode GSTs from four different classes: GST pi, sigma, mu and omega. A phylogenetic analysis of GST was made to determine the position of oyster GST compared to invertebrate and vertebrate sequences. We developed a semi-quantitative, multiplex RT-PCR to follow the expression of these four GSTs in tissues of oysters exposed to hydrocarbons and two pesticide treatments ( glyphosate and a mixture composed of atrazine, diuron and isoproturon) under experimental...
Tipo: Text Palavras-chave: Crassostrea gigas; Gene expression; Multiplex RTPCR; Pesticides; Hydrocarbons; CDNA sequences; Glutathione S transferases.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-431.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas ArchiMer
Tanguy, Arnaud; Boutet, Isabelle; Boudry, Pierre; Degremont, Lionel; Laroche, Jean; Moraga, Dario.
Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA...
Tipo: Text Palavras-chave: Gene expression; Xenobiotics; Mollusc; Phosphoglucomutase.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2220.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas ArchiMer
Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of...
Tipo: Text Palavras-chave: Crassostrea gigas; Flavin containing monooxygenase 2; Monoamine oxidase A.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-676.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature ArchiMer
Boutet, Isabelle; Jollivet, Didier; Shillito, Bruce; Moraga, Dario; Tanguy, Arnaud.
Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected...
Tipo: Text Palavras-chave: Oyster crassostrea gigas; Heat shock protein; Mid atlantic ridge; Cytosolic malate dehydrogenases; Extracellular hemoglobins; Lipid peroxidation; Northeast pacific; Enzyme activities; Arginine kinase; Dual symbiosis.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00129/24014/21970.pdf
Imagem não selecionada

Imprime registro no formato completo
Phylogéographie des huîtres de mangrove de l'Océan Atlantique Sud : C. gasar et C. rhisophorae ArchiMer
Heurtebise, Serge; Boutet, Isabelle; Verden, B.; Lapegue, Sylvie; Leitao, Alexandra; Thiriot-quievreux, Catherine; Garica, P.; Boudry, Pierre.
Mangrove oysters include various species that are also present on different coasts. In the southern part of the Atlantic Ocean, Crassostrea rhizophorae has been observed along the southern coast of America and Crassostrea gasar along the western coast of Africa. Fifteen populations of these oysters were sampled from these two geographic areas. Their polymorphism was studied on the 16S mitochondrial fragment both by par sequencing and RFLP analysis. Some samples' caryotype were also analysed. Two haplotypes were identified: C. gasar was found in Africa, but also for the first time in South America, whereas C. rhizophorae was only found on the coasts of South America.
Tipo: Text Palavras-chave: Crassostrea rhizophorae; Crassostrea gasar; Huîtres; Génétique; Phylogéographie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3278.pdf
Imagem não selecionada

Imprime registro no formato completo
Phylogeography of mangrove oysters from the Southern Atlantic ocean : Crassostrea gasar and Crassostre rhizophorae ArchiMer
Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Thiriot-quievreux, Catherine; Heurtebise, Serge; Garcia, P.; Boudry, Pierre.
Mangrove oysters encompass several species and their taxomony is unclear. Among then, Crassostrea rhizophorae has been described along the Central and South American Atlantic coasts and Crassostrea gasar along African Atlantic coasts. Most taxonomic studies have so far been based on morphological criteria which are particularly susceptible to plasticity and new insight can be provided by molecular markers. The present work aims to provide information on the phylogeography of these two taxa.
Tipo: Text Palavras-chave: Genetic; Phylogeography; Crassostrea rhizophorae; Crassostrea gasar; Mangrove oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3496.pdf
Imagem não selecionada

Imprime registro no formato completo
Proteome Adaptation to High Temperatures in the Ectothermic Hydrothermal Vent Pompeii Worm ArchiMer
Jollivet, Didier; Mary, Jean; Gagniere, Nicolas; Tanguy, Arnaud; Fontanillas, Eric; Boutet, Isabelle; Hourdez, Stephane; Segurens, Beatrice; Weissenbach, Jean; Poch, Olivier; Lecompte, Odile.
Taking advantage of the massive genome sequencing effort made on thermophilic prokaryotes, thermal adaptation has been extensively studied by analysing amino acid replacements and codon usage in these unicellular organisms. In most cases, adaptation to thermophily is associated with greater residue hydrophobicity and more charged residues. Both of these characteristics are positively correlated with the optimal growth temperature of prokaryotes. In contrast, little information has been collected on the molecular 'adaptive' strategy of thermophilic eukaryotes. The Pompeii worm A. pompejana, whose transcriptome has recently been sequenced, is currently considered as the most thermotolerant eukaryote on Earth, withstanding the greatest thermal and chemical...
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00129/24008/21976.pdf
Imagem não selecionada

Imprime registro no formato completo
Taxonomie et phylogéographie des huîtres creuses dans l'Atlantique Sud ArchiMer
Boutet, Isabelle.
L'étude de la taxonomie des huîtres est longtemps restée basée sur des critères morphologiques (forme de la coquille et description des organes) et géographiques. Ces considérations sont relativement peu fiables en particulier au niveau interspécifique du fait de la plasticité des caractères morphologiques d'une part de des introductions d'espèces étrangères sur les lieux de culture d'autre part. La biologie moléculaire est donc, depuis quelques années, un outil très utilisé pour différencier les espèces d'huîtres. La phylogéographie des huîtres de la région sud-atlantique a ainsi pu être étudiée grâce à différentes méthodes de recherche du polymorphisme réalisées sur le fragment mitochondrial 16S. La PCR-RFLP, la SSCP et le séquençage ont été utilisés...
Tipo: Text Palavras-chave: Taxonomie; Huîtres creuses; Crassostrea; Phylogéographie; ADN.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00032/14365/11656.pdf
Imagem não selecionada

Imprime registro no formato completo
Trans-Atlantic distribution of a mangrove oyster species revealed by 16S mtDNA and karyological analyses ArchiMer
Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre.
Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been...
Tipo: Text Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf
Registros recuperados: 13
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional